Restriction map for pF1KB8662
 (Linear) (Six-base) MAPSORT of: KIBB8662.seq  Check: 9421  from: 1  to: 1131



KIBB8662.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

ApoI r'AATT_y

Cuts at:      0     410    1131 
   Size:        410     721
 
BanI G'GyrC_C

Cuts at:      0     951    1131 
   Size:        951     180
 
BanII G_rGCy'C

Cuts at:      0      98     167    1131 
   Size:         98      69     964

  Fragments arranged by size: 

                 964      98      69
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     420     454    1131 
   Size:        420      34     677

  Fragments arranged by size: 

                 677     420      34
 
BglII A'GATC_T

Cuts at:      0     830     983    1131 
   Size:        830     153     148

  Fragments arranged by size: 

                 830     153     148
 
BlpI GC'TnA_GC

Cuts at:      0      97    1131 
   Size:         97    1034
 
Bme1580I G_kGCm'C

Cuts at:      0     954    1131 
   Size:        954     177
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     181    1021    1131 
   Size:         76     105     840     110

  Fragments arranged by size: 

                 840     110     105      76
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     718    1131 
   Size:        322     396     413

  Fragments arranged by size: 

                 413     396     322
 
BsaBI GATnn'nnATC

Cuts at:      0     249    1131 
   Size:        249     882
 
BsaWI w'CCGG_w

Cuts at:      0     926    1131 
   Size:        926     205
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     106     415     901    1131 
   Size:        106     309     486     230

  Fragments arranged by size: 

                 486     309     230     106
 
BseYI C'CCAG_C

Cuts at:      0     731    1123    1131 
   Size:        731     392       8

  Fragments arranged by size: 

                 731     392       8
 
BsiHKAI G_wGCw'C

Cuts at:      0      98    1131 
   Size:         98    1033
 
Bsp1286I G_dGCh'C

Cuts at:      0      98     167     954    1131 
   Size:         98      69     787     177

  Fragments arranged by size: 

                 787     177      98      69
 
BspEI T'CCGG_A

Cuts at:      0     926    1131 
   Size:        926     205
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     383     753    1131 
   Size:        383     370     378

  Fragments arranged by size: 

                 383     378     370
 
BsrDI GCAATG_nn'

Cuts at:      0     806    1131 
   Size:        806     325
 
BssSI C'ACGA_G

Cuts at:      0     385    1131 
   Size:        385     746
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     220    1131 
   Size:        220     911
 
BstYI r'GATC_y

Cuts at:      0     244     830     983    1131 
   Size:        244     586     153     148

  Fragments arranged by size: 

                 586     244     153     148
 
BtgI C'CryG_G

Cuts at:      0     270    1131 
   Size:        270     861
 
BtsI GCAGTG_nn'

Cuts at:      0     917    1131 
   Size:        917     214
 
EaeI y'GGCC_r

Cuts at:      0     497     938    1131 
   Size:        497     441     193

  Fragments arranged by size: 

                 497     441     193
 
EarI CTCTTCn'nnn_

Cuts at:      0     626    1000    1131 
   Size:        626     374     131

  Fragments arranged by size: 

                 626     374     131
 
EcoICRI GAG'CTC

Cuts at:      0      96    1131 
   Size:         96    1035
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     181    1021    1131 
   Size:         76     105     840     110

  Fragments arranged by size: 

                 840     110     105      76
 
EcoO109I rG'GnC_Cy

Cuts at:      0     645    1131 
   Size:        645     486
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     357     849     868    1131 
   Size:        357     492      19     263

  Fragments arranged by size: 

                 492     357     263      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      30    1131 
   Size:         30    1101
 
HindIII A'AGCT_T

Cuts at:      0     787    1131 
   Size:        787     344
 
MfeI C'AATT_G

Cuts at:      0     616     704    1131 
   Size:        616      88     427

  Fragments arranged by size: 

                 616     427      88
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      29      56    1131 
   Size:         29      27    1075

  Fragments arranged by size: 

                1075      29      27
 
MscI TGG'CCA

Cuts at:      0     499    1131 
   Size:        499     632
 
MslI CAynn'nnrTG

Cuts at:      0    1015    1131 
   Size:       1015     116
 
MspA1I CmG'CkG

Cuts at:      0      75     936    1131 
   Size:         75     861     195

  Fragments arranged by size: 

                 861     195      75
 
PasI CC'CwG_GG

Cuts at:      0     167    1131 
   Size:        167     964
 
PfoI T'CCnGG_A

Cuts at:      0     253     974    1131 
   Size:        253     721     157

  Fragments arranged by size: 

                 721     253     157
 
PshAI GACnn'nnGTC

Cuts at:      0     970    1131 
   Size:        970     161
 
PstI C_TGCA'G

Cuts at:      0     380     764    1131 
   Size:        380     384     367

  Fragments arranged by size: 

                 384     380     367
 
PvuII CAG'CTG

Cuts at:      0      75     936    1131 
   Size:         75     861     195

  Fragments arranged by size: 

                 861     195      75
 
SacI G_AGCT'C

Cuts at:      0      98    1131 
   Size:         98    1033
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1000    1131 
   Size:       1000     131
 
SfcI C'TryA_G

Cuts at:      0     376     760    1131 
   Size:        376     384     371

  Fragments arranged by size: 

                 384     376     371
 
SmlI C'TyrA_G

Cuts at:      0     301     697    1131 
   Size:        301     396     434

  Fragments arranged by size: 

                 434     396     301
 
SspI AAT'ATT

Cuts at:      0     148    1131 
   Size:        148     983
 
StuI AGG'CCT

Cuts at:      0     281    1131 
   Size:        281     850
 

 Enzymes that do cut:

     ApoI     BanI    BanII     BcgI    BglII     BlpI Bme1580I     BpmI
    BpuEI    BsaBI    BsaWI    BseRI    BseYI  BsiHKAI Bsp1286I    BspEI
    BspMI    BsrDI    BssSI   BstAPI    BstYI     BtgI     BtsI     EaeI
     EarI  EcoICRI  Eco57MI EcoO109I     FalI    Hin4I  HindIII     MfeI
     MmeI     MscI     MslI   MspA1I     PasI     PfoI    PshAI     PstI
    PvuII     SacI     SapI     SfcI     SmlI     SspI     StuI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BmgBI
     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaHI    BsaXI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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