Restriction map for pF1KB8657
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AflII       
AgeI        AhdI        AocI        Aor51HI     ApaI        
AscI        Asp700I     AspEI       AspI        AviII       
AvrII       BamHI       BbrPI       BbvCI       BciVI       
BclI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaBI       BseAI       BsiEI       BsiWI       
BspDI       BspEI       BspHI       BsrDI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
ClaI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        HphI        MamI        
MfeI        MluI        MroI        MunI        NcoI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       Psp1406I    PvuI        RcaI        
SacI        SalI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        SspI        SwaI        Tth111I     XbaI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I       1055  GGTACC               G^GTACC
AcsI          160  AAATTT               [GA]^AATT[CT]
AflIII       1064  ACATGT               A^C[GA][CT]GT
Alw44I        814  GTGCAC               G^TGCAC
AlwI         1023  GATCC                GGATC(4/5)
AlwNI         379  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         814  GTGCAC               G^TGCAC
ApoI          160  AAATTT               [GA]^AATT[CT]
AseI          568  ATTAAT               AT^TAAT
AsnI          568  ATTAAT               AT^TAAT
Asp718I      1055  GGTACC               G^GTACC
AvaI          829  CTCGAG               C^[CT]CG[GA]G
BalI          263  TGGCCA               TGG^CCA
BanII         859  GAGCCC               G[GA]GC[CT]^C
BbsI          556  GTCTTC               GAAGAC(2/6)
BcnI          875  CCGGG                CC^[GC]GG
BpuAI         556  GTCTTC               GAAGAC(2/6)
BsaAI        1225  TACGTG               [CT]AC^GT[GA]
BsaI          808  GAGACC               GGTCTC(1/5)
BsaWI         151  TCCGGT               [AT]^CCGG[AT]
BsgI          550  GTGCAG               GTGCAG(16/14)
BsmBI         457  GAGACG               CGTCTC(1/5)
BsmI          205  GAATGC               GAATGC(1/-1)
BsoBI         829  CTCGAG               C^[CT]CG[GA]G
BspLU11I     1064  ACATGT               A^CATGT
BsrBI          18  GAGCGG               CCGCTC(-3/-3)
BsrI          621  ACTGG                ACTGG(1/-1)
CpoI          965  CGGTCCG              CG^G[AT]CCG
EarI           46  CTCTTC               CTCTTC(1/4)
EcoNI        1317  CCTGGATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       286  CCAAGG               C^C[AT][AT]GG
KpnI         1055  GGTACC               GGTAC^C
Ksp632I        46  CTCTTC               CTCTTC(1/4)
KspI         1027  CCGCGG               CCGC^GG
MaeII        1226  ACGT                 A^CGT
MluNI         263  TGGCCA               TGG^CCA
MscI          263  TGGCCA               TGG^CCA
NciI          875  CCGGG                CC^[GC]GG
NheI         1005  GCTAGC               G^CTAGC
PaeR7I        829  CTCGAG               C^TCGAG
PflMI         863  CCACCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1126  GACGGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         568  ATTAAT               AT^TAAT
RsrII         965  CGGTCCG              CG^G[AT]CCG
SacII        1027  CCGCGG               CCGC^GG
SapI           45  GCTCTTC              GCTCTTC(1/4)
SbfI          472  CCTGCAGG             CCTGCA^GG
SmlI          829  CTCGAG               C^T[CT][GA]AG
Sse8387I      472  CCTGCAGG             CCTGCA^GG
StuI          477  AGGCCT               AGG^CCT
StyI          286  CCAAGG               C^C[AT][AT]GG
Van91I        863  CCACCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          829  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BbeI           31  GGCGCC               GGCGC^C
             1282  GGCGCC              
BcgI         1263  CGACCTGGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              950  GCAGAGCCTTCG        
BspMI         914  ACCTGC               ACCTGC(4/8)
              552  GCAGGT              
HgaI          459  GACGC                GACGC(5/10)
              631  GACGC               
KasI           31  GGCGCC               G^GCGCC
             1282  GGCGCC              
MaeIII        928  GTGAC                ^GT[ACGT]AC
             1186  GTCAC               
MslI           72  CACAGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              432  CATGAAGGTG          
NaeI           28  GCCGGC               GCC^GGC
               34  GCCGGC              
NarI           31  GGCGCC               GG^CGCC
             1282  GGCGCC              
NgoMIV         28  GCCGGC               G^CCGGC
               34  GCCGGC              
NspI          779  GCATGT               [GA]CATG^[CT]
             1064  ACATGT              
PleI         1159  GACTC                GAGTC(4/5)
              403  GAGTC               
PstI          473  CTGCAG               CTGCA^G
             1208  CTGCAG              
SfaNI          88  GCATC                GCATC(5/9)
              749  GCATC               
SfoI           31  GGCGCC               GGC^GCC
             1282  GGCGCC              
TaqI          560  TCGA                 T^CGA
              830  TCGA                
Tsp45I        928  GTGAC                ^GT[GC]AC
             1186  GTCAC               
TspRI         620  CACTG                CA[GC]TG[ACGT][ACGT]^
              804  CACTG               
TthHB8I       560  TCGA                 T^CGA
              830  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AspHI       AvaII       BanI        BbvI        BfaI        
BglII       BmyI        BpmI        BsaHI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    Bsp1407I    BsrFI       BsrGI       
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DsaI        EaeI        EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        Hin1I       HinP1I      HinfI       HpaII       
ItaI        MaeI        MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfcI        SspBI       TfiI        Tru9I       TseI        
Tsp509I     XcmI        XhoII       
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