Restriction map for pF1KB8645
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        AocI        Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BfaI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaBI       BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BstAPI      
BstBI       BstEII      BstPI       BstXI       Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HindIII     HpaI        KasI        KpnI        MaeI        
MamI        MfeI        MluI        MroI        MseI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SalI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tru9I       Tsp509I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI          175  GTATAC               GT^[AC][GT]AC
AflIII        194  ACATGT               A^C[GA][CT]GT
Alw44I        786  GTGCAC               G^TGCAC
AlwI          345  GATCC                GGATC(4/5)
AlwNI         343  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         786  GTGCAC               G^TGCAC
AspI            9  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           83  CTCGGG               C^[CT]CG[GA]G
AviII         248  TGCGCA               TGC^GCA
BalI          566  TGGCCA               TGG^CCA
BciVI         216  GGATAC               GTATCC(6/5)
BcnI          306  CCGGG                CC^[GC]GG
BglII         608  AGATCT               A^GATCT
BpmI          349  CTGGAG               CTGGAG(16/14)
BsaAI         550  TACGTG               [CT]AC^GT[GA]
BsaI          690  GGTCTC               GGTCTC(1/5)
BsmFI         779  GGGAC                GGGAC(10/14)
BsoBI          83  CTCGGG               C^[CT]CG[GA]G
BspLU11I      194  ACATGT               A^CATGT
BsrI          138  CCAGT                ACTGG(1/-1)
BssSI         580  CACGAG               CACGAG(-5/-1)
Bst1107I      175  GTATAC               GTA^TAC
BstZ17I       175  GTATAC               GTA^TAC
DdeI           34  CTCAG                C^T[ACGT]AG
EarI          556  CTCTTC               CTCTTC(1/4)
FspI          248  TGCGCA               TGC^GCA
HaeII         329  AGCGCC               [GA]GCGC^[CT]
HapII         306  CCGG                 C^CGG
HincII        718  GTCAAC               GT[CT]^[GA]AC
HindII        718  GTCAAC               GT[CT]^[GA]AC
HinfI         310  GAGTC                G^A[ACGT]TC
HpaII         306  CCGG                 C^CGG
HphI          625  GGTGA                GGTGA(8/7)
Ksp632I       556  CTCTTC               CTCTTC(1/4)
KspI          186  CCGCGG               CCGC^GG
MluNI         566  TGGCCA               TGG^CCA
MscI          566  TGGCCA               TGG^CCA
MspI          306  CCGG                 C^CGG
NciI          306  CCGGG                CC^[GC]GG
NcoI          401  CCATGG               C^CATGG
PflFI           9  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          310  GAGTC                GAGTC(4/5)
PpuMI         749  AGGTCCT              [GA]G^G[AT]CC[CT]
SacI           96  GAGCTC               GAGCT^C
SacII         186  CCGCGG               CCGC^GG
SapI          555  GCTCTTC              GCTCTTC(1/4)
SfaNI         600  GCATC                GCATC(5/9)
SmlI          730  CTCAAG               C^T[CT][GA]AG
SphI          653  GCATGC               GCATG^C
Tth111I         9  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII         187  CGCG                 CG^CG
              661  CGCG                
AcyI          628  GACGCC               G[GA]^CG[CT]C
              676  GACGCC              
AvaII         135  GGACC                G^G[AT]CC
              750  GGTCC               
BanI           76  GGTGCC               G^G[CT][GA]CC
              450  GGTGCC              
BanII          96  GAGCTC               G[GA]GC[CT]^C
              588  GAGCCC              
BbsI            6  GAAGAC               GAAGAC(2/6)
              277  GTCTTC              
BpuAI           6  GAAGAC               GAAGAC(2/6)
              277  GTCTTC              
BsaHI         628  GACGCC               G[GA]^CG[CT]C
              676  GACGCC              
BsgI           27  CTGCAC               GTGCAG(16/14)
              616  GTGCAG              
BsmAI         108  GTCTC                GTCTC(1/5)
              691  GTCTC               
BstUI         187  CGCG                 CG^CG
              661  CGCG                
BstYI         344  AGATCC               [GA]^GATC[CT]
              608  AGATCT              
DpnII         345  GATC                 ^GATC
              609  GATC                
DraII         643  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              749  AGGTCCT             
EcoO109I      643  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              749  AGGTCCT             
Hin1I         628  GACGCC               G[GA]^CG[CT]C
              676  GACGCC              
MaeII         533  ACGT                 A^CGT
              551  ACGT                
MaeIII        626  GTGAC                ^GT[ACGT]AC
              725  GTGAC               
MboI          345  GATC                 ^GATC
              609  GATC                
MflI          344  AGATCC               [GA]^GATC[CT]
              608  AGATCT              
MspA1I         18  CAGCGG               C[AC]G^C[GT]G
              186  CCGCGG              
MvnI          187  CGCG                 CG^CG
              661  CGCG                
NdeII         345  GATC                 ^GATC
              609  GATC                
NlaIV          76  GGTGCC               GG[ACGT]^[ACGT]CC
              450  GGTGCC              
NspI          194  ACATGT               [GA]CATG^[CT]
              653  GCATGC              
Sau3AI        345  GATC                 ^GATC
              609  GATC                
Tsp45I        626  GTGAC                ^GT[GC]AC
              725  GTGAC               
TspRI          36  CAGTG                CA[GC]TG[ACGT][ACGT]^
              723  CAGTG               
XhoII         344  AGATCC               [GA]^GATC[CT]
              608  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       BbvI        
BmyI        BsaJI       BsiHKAI     BsiYI       BslI        
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DsaI        EaeI        EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HgaI        HhaI        HinP1I      ItaI        MboII       
MnlI        MslI        MvaI        MwoI        NlaIII      
RsaI        Sau96I      ScrFI       StyI        TaqI        
TseI        TthHB8I     
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