Restriction map for pF1KB8634
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflII       
AflIII      AhdI        Alw44I      AocI        ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BpuAI       
BsaAI       BsaHI       BsaI        BseRI       BsiHKAI     
BsiWI       BsmBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
BstAPI      BstEII      BstPI       BstXI       Bsu36I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoO65I     EcoT22I     FauI        FbaI        
FseI        FspI        HgaI        Hin1I       HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MluNI       MscI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        PacI        PaeR7I      
PflFI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        TfiI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI          351  GTATAC               GT^[AC][GT]AC
AccIII         44  TCCGGA               T^CCGGA
AcsI          166  GAATTC               [GA]^AATT[CT]
AgeI          515  ACCGGT               A^CCGGT
Aor51HI       965  AGCGCT               AGC^GCT
ApoI          166  GAATTC               [GA]^AATT[CT]
Asp700I       439  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          188  CTCGGG               C^[CT]CG[GA]G
BseAI          44  TCCGGA               T^CCGGA
BsgI          977  GTGCAG               GTGCAG(16/14)
BsiEI         135  CGACCG               CG[GA][CT]^CG
BsmAI         248  GTCTC                GTCTC(1/5)
BsmI           51  GAATGC               GAATGC(1/-1)
BsoBI         188  CTCGGG               C^[CT]CG[GA]G
BspEI          44  TCCGGA               T^CCGGA
BspMI         119  ACCTGC               ACCTGC(4/8)
BssSI          15  CTCGTG               CACGAG(-5/-1)
Bst1107I      351  GTATAC               GTA^TAC
BstBI         456  TTCGAA               TT^CGAA
BstZ17I       351  GTATAC               GTA^TAC
CfoI          966  GCGC                 GCG^C
EarI          250  CTCTTC               CTCTTC(1/4)
Eco47III      965  AGCGCT               AGC^GCT
EcoNI         921  CCTCTGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         166  GAATTC               G^AATTC
EcoRV         616  GATATC               GAT^ATC
HaeII         965  AGCGCT               [GA]GCGC^[CT]
HhaI          966  GCGC                 GCG^C
HinP1I        966  GCGC                 G^CGC
HincII        335  GTCAAC               GT[CT]^[GA]AC
HindII        335  GTCAAC               GT[CT]^[GA]AC
HindIII       901  AAGCTT               A^AGCTT
Ksp632I       250  CTCTTC               CTCTTC(1/4)
MaeII         227  ACGT                 A^CGT
MaeIII        571  GTCAC                ^GT[ACGT]AC
MroI           44  TCCGGA               T^CCGGA
MspA1I        547  CAGCTG               C[AC]G^C[GT]G
MwoI          556  GCTGAATGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspV          456  TTCGAA               TT^CGAA
PflMI         671  CCAAGGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         515  ACCGGT               A^CCGGT
PvuII         547  CAGCTG               CAG^CTG
SfaNI         417  GATGC                GCATC(5/9)
SfuI          456  TTCGAA               TT^CGAA
SspI          201  AATATT               AAT^ATT
TaqI          457  TCGA                 T^CGA
Tsp45I        571  GTCAC                ^GT[GC]AC
TthHB8I       457  TCGA                 T^CGA
Van91I        671  CCAAGGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          566  CCATCGTCACCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          439  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AccII         100  CGCG                 CG^CG
              957  CGCG                
AfaI          582  GTAC                 GT^AC
              936  GTAC                
AlwNI         294  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              715  CAGATCCTG           
BanII         874  GAGCCC               G[GA]GC[CT]^C
              893  GGGCTC              
BfaI          316  CTAG                 C^TAG
              754  CTAG                
BlpI          544  GCTCAGC              GC^T[ACGT]AGC
              968  GCTCAGC             
BmyI          874  GAGCCC               G[AGT]GC[ACT]^C
              893  GGGCTC              
BpmI          269  CTCCAG               CTGGAG(16/14)
               67  CTGGAG              
Bpu1102I      544  GCTCAGC              GC^T[ACGT]AGC
              968  GCTCAGC             
BsaBI         436  GATGAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              612  GATAGATATC          
BsaWI          44  TCCGGA               [AT]^CCGG[AT]
              515  ACCGGT              
Bsp1286I      874  GAGCCC               G[AGT]GC[ACT]^C
              893  GGGCTC              
BsrFI          77  ACCGGC               [GA]^CCGG[CT]
              515  ACCGGT              
BstUI         100  CGCG                 CG^CG
              957  CGCG                
BstYI         716  AGATCC               [GA]^GATC[CT]
              952  AGATCC              
Cac8I         193  GCCAGC               GC[ACGT]^[ACGT]GC
              663  GCTGGC              
CelII         544  GCTCAGC              GC^T[ACGT]AGC
              968  GCTCAGC             
Cfr10I         77  ACCGGC               [GA]^CCGG[CT]
              515  ACCGGT              
DraII         401  GGGACCC              [GA]G^G[ACGT]CC[CT]
              941  AGGACCT             
EaeI           79  CGGCCA               [CT]^GGCC[GA]
              626  CGGCCA              
EcoO109I      401  GGGACCC              [GA]G^G[ACGT]CC[CT]
              941  AGGACCT             
EcoT14I       671  CCAAGG               C^C[AT][AT]GG
              869  CCTTGG              
HphI          447  TCACC                GGTGA(8/7)
              572  TCACC               
MaeI          316  CTAG                 C^TAG
              754  CTAG                
MamI          436  GATGAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              612  GATAGATATC          
MflI          716  AGATCC               [GA]^GATC[CT]
              952  AGATCC              
MslI          573  CACCCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              729  CATCACGGTG          
MvnI          100  CGCG                 CG^CG
              957  CGCG                
NlaIII        162  CATG                 CATG^
              744  CATG                
PpuMI         401  GGGACCC              [GA]G^G[AT]CC[CT]
              941  AGGACCT             
PstI          121  CTGCAG               CTGCA^G
              778  CTGCAG              
RsaI          582  GTAC                 GT^AC
              936  GTAC                
SfcI          121  CTGCAG               C^T[GA][CT]AG
              778  CTGCAG              
SmlI          346  CTCAAG               C^T[CT][GA]AG
              904  CTTGAG              
StyI          671  CCAAGG               C^C[AT][AT]GG
              869  CCTTGG              
XhoII         716  AGATCC               [GA]^GATC[CT]
              952  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BbvI        
BcnI        BsaJI       BsiYI       BslI        BsmFI       
BsrI        BssKI       BstF5I      BstNI       Cfr13I      
DdeI        DpnII       EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       ItaI        
MboI        MboII       MnlI        MseI        MspI        
MvaI        NciI        NdeII       NlaIV       PleI        
Sau3AI      Sau96I      ScrFI       Tru9I       TseI        
Tsp509I     TspRI       
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