Restriction map for pF1KB8633
 (Linear) (Six-base) MAPSORT of: KIBB8633.seq  Check: 6623  from: 1  to: 1506



KIBB8633.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

Acc65I G'GTAC_C

Cuts at:      0    1173    1506 
   Size:       1173     333
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     451    1506 
   Size:        451    1055
 
AfeI AGC'GCT

Cuts at:      0     940    1506 
   Size:        940     566
 
AflIII A'CryG_T

Cuts at:      0     152    1506 
   Size:        152    1354
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     437    1506 
   Size:        437    1069
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1102    1506 
   Size:       1102     404
 
ApoI r'AATT_y

Cuts at:      0     541    1506 
   Size:        541     965
 
BanI G'GyrC_C

Cuts at:      0       6     951    1173    1283    1506 
   Size:          6     945     222     110     223

  Fragments arranged by size: 

                 945     223     222     110       6
 
BanII G_rGCy'C

Cuts at:      0     842    1033    1209    1506 
   Size:        842     191     176     297

  Fragments arranged by size: 

                 842     297     191     176
 
BbeI G_GCGC'C

Cuts at:      0      10    1506 
   Size:         10    1496
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     637     638    1506 
   Size:        637       1     868

  Fragments arranged by size: 

                 868     637       1
 
BclI T'GATC_A

Cuts at:      0     675    1506 
   Size:        675     831
 
BfrBI ATG'CAT

Cuts at:      0     886    1506 
   Size:        886     620
 
BlpI GC'TnA_GC

Cuts at:      0     425    1506 
   Size:        425    1081
 
Bme1580I G_kGCm'C

Cuts at:      0    1288    1506 
   Size:       1288     218
 
BmgBI CAC'GTC

Cuts at:      0     155     735    1506 
   Size:        155     580     771

  Fragments arranged by size: 

                 771     580     155
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     162    1123    1506 
   Size:        162     961     383

  Fragments arranged by size: 

                 961     383     162
 
BmtI G_CTAG'C

Cuts at:      0    1109    1506 
   Size:       1109     397
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1228    1506 
   Size:       1228     278
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     565    1189    1206    1506 
   Size:        565     624      17     300

  Fragments arranged by size: 

                 624     565     300      17
 
Bpu10I CC'TnA_GC

Cuts at:      0    1091    1506 
   Size:       1091     415
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     943    1506 
   Size:        328     615     563

  Fragments arranged by size: 

                 615     563     328
 
BsaHI Gr'CG_yC

Cuts at:      0       7    1506 
   Size:          7    1499
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     953    1209    1506 
   Size:        953     256     297

  Fragments arranged by size: 

                 953     297     256
 
BseYI C'CCAG_C

Cuts at:      0     317     764    1061    1356    1506 
   Size:        317     447     297     295     150

  Fragments arranged by size: 

                 447     317     297     295     150
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77    1506 
   Size:         77    1429
 
BsiHKAI G_wGCw'C

Cuts at:      0     352    1209    1501    1506 
   Size:        352     857     292       5

  Fragments arranged by size: 

                 857     352     292       5
 
Bsp1286I G_dGCh'C

Cuts at:      0     352     842    1033    1209    1288    1501    1506 
   Size:        352     490     191     176      79     213       5

  Fragments arranged by size: 

                 490     352     213     191     176      79       5
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     262    1506 
   Size:        262    1244
 
BsrDI GCAATG_nn'

Cuts at:      0     826    1506 
   Size:        826     680
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1395    1506 
   Size:       1395     111
 
Bsu36I CC'TnA_GG

Cuts at:      0     595    1506 
   Size:        595     911
 
BtsI GCAGTG_nn'

Cuts at:      0     737    1385    1489    1506 
   Size:        737     648     104      17

  Fragments arranged by size: 

                 737     648     104      17
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     727    1506 
   Size:        727     779
 
EaeI y'GGCC_r

Cuts at:      0     472    1506 
   Size:        472    1034
 
EarI CTCTTCn'nnn_

Cuts at:      0     122    1074    1506 
   Size:        122     952     432

  Fragments arranged by size: 

                 952     432     122
 
EcoICRI GAG'CTC

Cuts at:      0    1207    1506 
   Size:       1207     299
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     451     565    1189    1206    1506 
   Size:        451     114     624      17     300

  Fragments arranged by size: 

                 624     451     300     114      17
 
EcoO109I rG'GnC_Cy

Cuts at:      0      80    1506 
   Size:         80    1426
 
EcoRI G'AATT_C

Cuts at:      0     541    1506 
   Size:        541     965
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     456     688    1506 
   Size:        456     232     818

  Fragments arranged by size: 

                 818     456     232
 
HaeII r_GCGC'y

Cuts at:      0      10     942    1355    1506 
   Size:         10     932     413     151

  Fragments arranged by size: 

                 932     413     151      10
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     469     501    1020    1052    1162    1194    1249    1281 
   Size:        469      32     519      32     110      32      55      32

Cuts at:   1281    1506 
   Size:        225

  Fragments arranged by size: 

                 519     469     225     110      55      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1083    1506 
   Size:       1083     423
 
HindIII A'AGCT_T

Cuts at:      0     362    1248    1506 
   Size:        362     886     258

  Fragments arranged by size: 

                 886     362     258
 
KasI G'GCGC_C

Cuts at:      0       6    1506 
   Size:          6    1500
 
KpnI G_GTAC'C

Cuts at:      0    1177    1506 
   Size:       1177     329
 
MscI TGG'CCA

Cuts at:      0     474    1506 
   Size:        474    1032
 
MspA1I CmG'CkG

Cuts at:      0     102    1423    1506 
   Size:        102    1321      83

  Fragments arranged by size: 

                1321     102      83
 
NarI GG'CG_CC

Cuts at:      0       7    1506 
   Size:          7    1499
 
NheI G'CTAG_C

Cuts at:      0    1105    1506 
   Size:       1105     401
 
NsiI A_TGCA'T

Cuts at:      0     888    1506 
   Size:        888     618
 
NspI r_CATG'y

Cuts at:      0     815    1506 
   Size:        815     691
 
PpuMI rG'GwC_Cy

Cuts at:      0      80    1506 
   Size:         80    1426
 
PvuII CAG'CTG

Cuts at:      0     102    1423    1506 
   Size:        102    1321      83

  Fragments arranged by size: 

                1321     102      83
 
SacI G_AGCT'C

Cuts at:      0    1209    1506 
   Size:       1209     297
 
SanDI GG'GwC_CC

Cuts at:      0      80    1506 
   Size:         80    1426
 
SfoI GGC'GCC

Cuts at:      0       8    1506 
   Size:          8    1498
 
SmlI C'TyrA_G

Cuts at:      0     343     922    1506 
   Size:        343     579     584

  Fragments arranged by size: 

                 584     579     343
 
SspI AAT'ATT

Cuts at:      0    1475    1506 
   Size:       1475      31
 
StuI AGG'CCT

Cuts at:      0     920    1506 
   Size:        920     586
 
StyI C'CwwG_G

Cuts at:      0    1288    1506 
   Size:       1288     218
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     509    1281    1506 
   Size:        509     772     225

  Fragments arranged by size: 

                 772     509     225
 
TatI w'GTAC_w

Cuts at:      0     445    1506 
   Size:        445    1061
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1480    1506 
   Size:       1480      26
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     175     876    1506 
   Size:        175     701     630

  Fragments arranged by size: 

                 701     630     175
 
XmnI GAAnn'nnTTC

Cuts at:      0    1465    1506 
   Size:       1465      41
 

 Enzymes that do cut:

   Acc65I     AcuI     AfeI   AflIII     AlfI    AlwNI     ApoI     BanI
    BanII     BbeI     BbsI     BclI    BfrBI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaHI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrDI    BstXI   Bsu36I
     BtsI     DrdI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MscI
   MspA1I     NarI     NheI     NsiI     NspI    PpuMI    PvuII     SacI
    SanDI     SfoI     SmlI     SspI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI    AflII     AgeI     AhdI     AleI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BbvCI     BcgI    BciVI     BglI    BglII     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI  BstZ17I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     EagI     EciI    EcoNI    EcoRV     FseI     FspI
    FspAI     HpaI     MfeI     MluI     MmeI     MslI     NaeI     NcoI
     NdeI   NgoMIV     NotI     NruI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SwaI  Tth111I     XbaI     XhoI     XmaI
     ZraI
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