Restriction map for pF1KB8632
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AhdI        Alw44I      
Aor51HI     ApaLI       AscI        Asp718I     AspEI       
AspHI       AviII       AvrII       BbeI        BbrPI       
BcgI        BciVI       BfrI        BglI        BglII       
BlnI        BsaAI       BsaBI       BsaHI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DraI        DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRV       FauI        FseI        FspI        Hin1I       
HindIII     HpaI        HphI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NspI        NspV        
PacI        PaeR7I      PmaCI       PmeI        PmlI        
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccII        1263  CGCG                 CG^CG
AgeI         1682  ACCGGT               A^CCGGT
AocI         2494  CCTGAGG              CC^T[ACGT]AGG
AseI         1809  ATTAAT               AT^TAAT
AsnI         1809  ATTAAT               AT^TAAT
AvaI         1889  CCCGAG               C^[CT]CG[GA]G
BanI          553  GGCACC               G^G[CT][GA]CC
BbsI          699  GTCTTC               GAAGAC(2/6)
BclI         1638  TGATCA               T^GATCA
BlpI         2455  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     2455  GCTAAGC              GC^T[ACGT]AGC
BpuAI         699  GTCTTC               GAAGAC(2/6)
BsaWI        1682  ACCGGT               [AT]^CCGG[AT]
BsgI         1727  GTGCAG               GTGCAG(16/14)
BsoBI        1889  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     1403  TGTACA               T^GTACA
BspHI        1839  TCATGA               T^CATGA
BsrFI        1682  ACCGGT               [GA]^CCGG[CT]
BsrGI        1403  TGTACA               T^GTACA
BstUI        1263  CGCG                 CG^CG
BstXI        1583  CCAAGAACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       2494  CCTGAGG              CC^T[ACGT]AGG
CelII        2455  GCTAAGC              GC^T[ACGT]AGC
CfoI          320  GCGC                 GCG^C
Cfr10I       1682  ACCGGT               [GA]^CCGG[CT]
DraIII       2063  CACATAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       2494  CCTGAGG              CC^T[ACGT]AGG
EcoT22I      1448  ATGCAT               ATGCA^T
FbaI         1638  TGATCA               T^GATCA
HaeII         319  GGCGCT               [GA]GCGC^[CT]
HgaI          697  GCGTC                GACGC(5/10)
HhaI          320  GCGC                 GCG^C
HinP1I        320  GCGC                 G^CGC
HincII       1108  GTTGAC               GT[CT]^[GA]AC
HindII       1108  GTTGAC               GT[CT]^[GA]AC
MaeII        2210  ACGT                 A^CGT
MslI         2030  CATTCATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI         1263  CGCG                 CG^CG
NdeI         2034  CATATG               CA^TATG
NsiI         1448  ATGCAT               ATGCA^T
PflMI         234  CCAAGTAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1682  ACCGGT               A^CCGGT
PshAI         723  GACAAGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1809  ATTAAT               AT^TAAT
PstI         1749  CTGCAG               CTGCA^G
RcaI         1839  TCATGA               T^CATGA
SapI         1612  GAAGAGC              GCTCTTC(1/4)
SpeI          522  ACTAGT               A^CTAGT
SspBI        1403  TGTACA               T^GTACA
Van91I        234  CCAAGTAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1954  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI         1266  GTAC                 GT^AC
             1404  GTAC                
AlwNI        1232  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1730  CAGAGTCTG           
ApaI          386  GGGCCC               GGGCC^C
             1094  GGGCCC              
Asp700I       541  GAAACCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1871  GAAGTGCTTC          
AspI          159  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1474  GACGAGGTC           
BamHI        1258  GGATCC               G^GATCC
             1827  GGATCC              
BanII         386  GGGCCC               G[GA]GC[CT]^C
             1094  GGGCCC              
BbvCI        1273  GCTGAGG              CCTCAGC(-5/-2)
             1435  GCTGAGG             
BcnI         1692  CCGGG                CC^[GC]GG
             2162  CCGGG               
BsrDI         202  GCAATG               GCAATG(2/0)
             1858  GCAATG              
MwoI         1264  GCGTACTGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1635  GCCTGATCAGC         
NciI         1692  CCGGG                CC^[GC]GG
             2162  CCGGG               
PflFI         159  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1474  GACGAGGTC           
RsaI         1266  GTAC                 GT^AC
             1404  GTAC                
SexAI         760  ACCAGGT              A^CC[AT]GGT
             1497  ACCTGGT             
SfcI          692  CTACAG               C^T[GA][CT]AG
             1749  CTGCAG              
SspI         2286  AATATT               AAT^ATT
             2326  AATATT              
StuI          650  AGGCCT               AGG^CCT
             1373  AGGCCT              
TaqI         1701  TCGA                 T^CGA
             1902  TCGA                
Tsp45I        417  GTCAC                ^GT[GC]AC
             1480  GTCAC               
Tth111I       159  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1474  GACGAGGTC           
TthHB8I      1701  TCGA                 T^CGA
             1902  TCGA                
XmnI          541  GAAACCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1871  GAAGTGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
AvaII       BalI        BbvI        BfaI        BmyI        
BpmI        BsaI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoO109I    EcoRI       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MspA1I      MspI        MvaI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
SmlI        StyI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       XcmI        XhoII       
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