Restriction map for pF1KB8632
 (Linear) (Six-base) MAPSORT of: KIBB8632.seq  Check: 5295  from: 1  to: 2514



KIBB8632.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     428    1010    1193    1349    1615    1805    2514 
   Size:        428     582     183     156     266     190     709

  Fragments arranged by size: 

                 709     582     428     266     190     183     156
 
AgeI A'CCGG_T

Cuts at:      0    1682    2514 
   Size:       1682     832
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1237    1735    2514 
   Size:       1237     498     779

  Fragments arranged by size: 

                1237     779     498
 
ApaI G_GGCC'C

Cuts at:      0     390    1098    2514 
   Size:        390     708    1416

  Fragments arranged by size: 

                1416     708     390
 
ApoI r'AATT_y

Cuts at:      0     397    1489    1544    1697    1798    1820    2229    2440 
   Size:        397    1092      55     153     101      22     409     211

Cuts at:   2440    2514 
   Size:         74

  Fragments arranged by size: 

                1092     409     397     211     153     101      74      55
                  22
 
AseI AT'TA_AT

Cuts at:      0    1810    2514 
   Size:       1810     704
 
AvaI C'yCGr_G

Cuts at:      0    1889    2514 
   Size:       1889     625
 
BamHI G'GATC_C

Cuts at:      0    1258    1827    2514 
   Size:       1258     569     687

  Fragments arranged by size: 

                1258     687     569
 
BanI G'GyrC_C

Cuts at:      0     553    2514 
   Size:        553    1961
 
BanII G_rGCy'C

Cuts at:      0     390    1098    2514 
   Size:        390     708    1416

  Fragments arranged by size: 

                1416     708     390
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     692    2514 
   Size:        692    1822
 
BbvCI CC'TCA_GC

Cuts at:      0    1274    1436    2514 
   Size:       1274     162    1078

  Fragments arranged by size: 

                1274    1078     162
 
BclI T'GATC_A

Cuts at:      0    1638    2514 
   Size:       1638     876
 
BfrBI ATG'CAT

Cuts at:      0    1450    2514 
   Size:       1450    1064
 
BlpI GC'TnA_GC

Cuts at:      0    2456    2514 
   Size:       2456      58
 
Bme1580I G_kGCm'C

Cuts at:      0      30     390    1098    2514 
   Size:         30     360     708    1416

  Fragments arranged by size: 

                1416     708     360      30
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      14     146    1278    2514 
   Size:         14     132    1132    1236

  Fragments arranged by size: 

                1236    1132     132      14
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     669    2514 
   Size:        669    1845
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     734    1046    1440    2514 
   Size:        374     360     312     394    1074

  Fragments arranged by size: 

                1074     394     374     360     312
 
Bpu10I CC'TnA_GC

Cuts at:      0     952    1274    1436    2514 
   Size:        952     322     162    1078

  Fragments arranged by size: 

                1078     952     322     162
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254    1591    2101    2434    2514 
   Size:        254    1337     510     333      80

  Fragments arranged by size: 

                1337     510     333     254      80
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     151     679    2302    2514 
   Size:        151     528    1623     212

  Fragments arranged by size: 

                1623     528     212     151
 
BsaWI w'CCGG_w

Cuts at:      0    1682    2514 
   Size:       1682     832
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     859     889    2514 
   Size:        859      30    1625

  Fragments arranged by size: 

                1625     859      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     613     661     943    1159    1255    1453    2461    2514 
   Size:        613      48     282     216      96     198    1008      53

  Fragments arranged by size: 

                1008     613     282     216     198      96      53      48
            
 
BseYI C'CCAG_C

Cuts at:      0      52      70    2514 
   Size:         52      18    2444

  Fragments arranged by size: 

                2444      52      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1748    2514 
   Size:       1748     766
 
Bsp1286I G_dGCh'C

Cuts at:      0      30     390    1098    2514 
   Size:         30     360     708    1416

  Fragments arranged by size: 

                1416     708     360      30
 
BspHI T'CATG_A

Cuts at:      0    1839    2514 
   Size:       1839     675
 
BsrDI GCAATG_nn'

Cuts at:      0     209    1865    2514 
   Size:        209    1656     649

  Fragments arranged by size: 

                1656     649     209
 
BsrFI r'CCGG_y

Cuts at:      0    1682    2514 
   Size:       1682     832
 
BsrGI T'GTAC_A

Cuts at:      0    1403    2514 
   Size:       1403    1111
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1590    2514 
   Size:       1590     924
 
BstYI r'GATC_y

Cuts at:      0     811     847    1068    1258    1827    2514 
   Size:        811      36     221     190     569     687

  Fragments arranged by size: 

                 811     687     569     221     190      36
 
Bsu36I CC'TnA_GG

Cuts at:      0    2495    2514 
   Size:       2495      19
 
BtsI GCAGTG_nn'

Cuts at:      0     419     467    2514 
   Size:        419      48    2047

  Fragments arranged by size: 

                2047     419      48
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2273    2308    2514 
   Size:       2273      35     206

  Fragments arranged by size: 

                2273     206      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2068    2514 
   Size:       2068     446
 
EaeI y'GGCC_r

Cuts at:      0     457     464    1301    2514 
   Size:        457       7     837    1213

  Fragments arranged by size: 

                1213     837     457       7
 
EarI CTCTTCn'nnn_

Cuts at:      0      53     713     743    1025    1304    1328    1547    1607 
   Size:         53     660      30     282     279      24     219      60

Cuts at:   1607    1781    2306    2514 
   Size:        174     525     208

  Fragments arranged by size: 

                 660     525     282     279     219     208     174      60
                  53      30      24
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1271    2514 
   Size:       1271    1243
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     428     734    1010    1046    1193    1349    1440 
   Size:        374      54     306     276      36     147     156      91

Cuts at:   1440    1615    1805    2514 
   Size:        175     190     709

  Fragments arranged by size: 

                 709     374     306     276     190     175     156     147
                  91      54      36
 
EcoO109I rG'GnC_Cy

Cuts at:      0     122     501    1094    1163    1349    1460    2514 
   Size:        122     379     593      69     186     111    1054

  Fragments arranged by size: 

                1054     593     379     186     122     111      69
 
EcoRI G'AATT_C

Cuts at:      0    1489    1544    1798    2514 
   Size:       1489      55     254     716

  Fragments arranged by size: 

                1489     716     254      55
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1819    2406    2514 
   Size:       1819     587     108

  Fragments arranged by size: 

                1819     587     108
 
HaeII r_GCGC'y

Cuts at:      0     323    2514 
   Size:        323    2191
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     148     180     466     498     509     541     673     705 
   Size:        148      32     286      32      11      32     132      32

Cuts at:    705     826     858    1267    1299    1606    1638    1729    1761 
   Size:        121      32     409      32     307      32      91      32

Cuts at:   1761    2514 
   Size:        753

  Fragments arranged by size: 

                 753     409     307     286     148     132     121      91
                  32      32      32      32      32      32      32      32
                  11
 
HincII GTy'rAC

Cuts at:      0    1110    2514 
   Size:       1110    1404
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     867    2514 
   Size:        867    1647
 
MscI TGG'CCA

Cuts at:      0     459     466    1303    2514 
   Size:        459       7     837    1211

  Fragments arranged by size: 

                1211     837     459       7
 
MslI CAynn'nnrTG

Cuts at:      0    2034    2514 
   Size:       2034     480
 
MspA1I CmG'CkG

Cuts at:      0    1435    1754    2088    2514 
   Size:       1435     319     334     426

  Fragments arranged by size: 

                1435     426     334     319
 
NdeI CA'TA_TG

Cuts at:      0    2035    2514 
   Size:       2035     479
 
NsiI A_TGCA'T

Cuts at:      0    1452    2514 
   Size:       1452    1062
 
PasI CC'CwG_GG

Cuts at:      0     558     635     743    2514 
   Size:        558      77     108    1771

  Fragments arranged by size: 

                1771     558     108      77
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     240    2514 
   Size:        240    2274
 
PfoI T'CCnGG_A

Cuts at:      0    1691    2514 
   Size:       1691     823
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     652     684    2514 
   Size:        652      32    1830

  Fragments arranged by size: 

                1830     652      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     122    1163    1349    2514 
   Size:        122    1041     186    1165

  Fragments arranged by size: 

                1165    1041     186     122
 
PshAI GACnn'nnGTC

Cuts at:      0     727    2514 
   Size:        727    1787
 
PsiI TTA'TAA

Cuts at:      0    2371    2514 
   Size:       2371     143
 
PspOMI G'GGCC_C

Cuts at:      0     386    1094    2514 
   Size:        386     708    1420

  Fragments arranged by size: 

                1420     708     386
 
PstI C_TGCA'G

Cuts at:      0    1753    2514 
   Size:       1753     761
 
PvuII CAG'CTG

Cuts at:      0    1435    1754    2088    2514 
   Size:       1435     319     334     426

  Fragments arranged by size: 

                1435     426     334     319
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1607    2514 
   Size:       1607     907
 
SexAI A'CCwGG_T

Cuts at:      0     760    1497    2514 
   Size:        760     737    1017

  Fragments arranged by size: 

                1017     760     737
 
SfcI C'TryA_G

Cuts at:      0     692    1749    2514 
   Size:        692    1057     765

  Fragments arranged by size: 

                1057     765     692
 
SmlI C'TyrA_G

Cuts at:      0     269    1570    2080    2413    2514 
   Size:        269    1301     510     333     101

  Fragments arranged by size: 

                1301     510     333     269     101
 
SpeI A'CTAG_T

Cuts at:      0     522    2514 
   Size:        522    1992
 
SspI AAT'ATT

Cuts at:      0    2288    2328    2514 
   Size:       2288      40     186

  Fragments arranged by size: 

                2288     186      40
 
StuI AGG'CCT

Cuts at:      0     652    1375    2514 
   Size:        652     723    1139

  Fragments arranged by size: 

                1139     723     652
 
StyI C'CwwG_G

Cuts at:      0     113     341     365    2514 
   Size:        113     228      24    2149

  Fragments arranged by size: 

                2149     228     113      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     232     571    2514 
   Size:        232     339    1943

  Fragments arranged by size: 

                1943     339     232
 
TatI w'GTAC_w

Cuts at:      0    1403    2514 
   Size:       1403    1111
 
Tth111I GACn'n_nGTC

Cuts at:      0     162    1477    2514 
   Size:        162    1315    1037

  Fragments arranged by size: 

                1315    1037     162
 
XbaI T'CTAG_A

Cuts at:      0    1954    2514 
   Size:       1954     560
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     133     205     241     397     583     878    2514 
   Size:        133      72      36     156     186     295    1636

  Fragments arranged by size: 

                1636     295     186     156     133      72      36
 
XmnI GAAnn'nnTTC

Cuts at:      0     545    1875    2514 
   Size:        545    1330     639

  Fragments arranged by size: 

                1330     639     545
 

 Enzymes that do cut:

     AcuI     AgeI    AlwNI     ApaI     ApoI     AseI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI     BclI    BfrBI     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BsaXI
    BseRI    BseYI     BsgI Bsp1286I    BspHI    BsrDI    BsrFI    BsrGI
    BstXI    BstYI   Bsu36I     BtsI    CspCI   DraIII     EaeI     EarI
     EciI  Eco57MI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII
     MmeI     MscI     MslI   MspA1I     NdeI     NsiI     PasI    PflMI
     PfoI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI    PvuII
     SapI    SexAI     SfcI     SmlI     SpeI     SspI     StuI     StyI
    TaqII     TatI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI     AloI    ApaLI     AscI    AsiSI    AvrII
     BaeI     BbeI     BcgI    BciVI     BglI    BglII    BmgBI     BmtI
    BsaAI    BsaBI    BsaHI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I     BtgI    BtgZI     ClaI     DraI     DrdI     EagI  EcoICRI
    EcoNI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NspI     PacI     PciI     PmeI     PmlI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI
     SrfI     SwaI     TstI     XhoI     XmaI     ZraI
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