Restriction map for
pF1KB8631
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvrII BanI
BbeI BbrPI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HaeII Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SgrAI SmaI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 638 AAATTT [GA]^AATT[CT]
AocI 651 CCTTAGG CC^T[ACGT]AGG
ApoI 638 AAATTT [GA]^AATT[CT]
AvaI 176 CCCGAG C^[CT]CG[GA]G
AviII 725 TGCGCA TGC^GCA
BamHI 225 GGATCC G^GATCC
BbsI 100 GTCTTC GAAGAC(2/6)
BbvCI 1306 CCTCAGC CCTCAGC(-5/-2)
BcnI 82 CCGGG CC^[GC]GG
BpmI 341 CTCCAG CTGGAG(16/14)
BpuAI 100 GTCTTC GAAGAC(2/6)
BsaAI 1096 TACGTA [CT]AC^GT[GA]
BseRI 568 CTCCTC GAGGAG(10/8)
BsgI 514 CTGCAC GTGCAG(16/14)
BsoBI 176 CCCGAG C^[CT]CG[GA]G
BsrDI 127 GCAATG GCAATG(2/0)
BstAPI 516 GCACTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 888 TTCGAA TT^CGAA
Bsu36I 651 CCTTAGG CC^T[ACGT]AGG
CfoI 726 GCGC GCG^C
DsaI 113 CCGTGG C^C[GA][CT]GG
Eco81I 651 CCTTAGG CC^T[ACGT]AGG
FspI 725 TGCGCA TGC^GCA
HgaI 1070 GCGTC GACGC(5/10)
HhaI 726 GCGC GCG^C
HinP1I 726 GCGC G^CGC
HincII 802 GTCAAC GT[CT]^[GA]AC
HindII 802 GTCAAC GT[CT]^[GA]AC
MaeII 1097 ACGT A^CGT
MaeIII 619 GTGAC ^GT[ACGT]AC
NciI 82 CCGGG CC^[GC]GG
NspI 722 GCATGC [GA]CATG^[CT]
NspV 888 TTCGAA TT^CGAA
ScaI 1046 AGTACT AGT^ACT
SfaNI 187 GATGC GCATC(5/9)
SfuI 888 TTCGAA TT^CGAA
SnaBI 1096 TACGTA TAC^GTA
SphI 722 GCATGC GCATG^C
Tsp45I 619 GTGAC ^GT[GC]AC
XcmI 206 CCAGAGCCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BalI 288 TGGCCA TGG^CCA
1207 TGGCCA
BsaI 4 GAGACC GGTCTC(1/5)
784 GAGACC
BspMI 512 ACCTGC ACCTGC(4/8)
1371 ACCTGC
BsrFI 7 ACCGGC [GA]^CCGG[CT]
493 ACCGGC
Cfr10I 7 ACCGGC [GA]^CCGG[CT]
493 ACCGGC
DraII 180 AGGTCCC [GA]G^G[ACGT]CC[CT]
280 AGGACCT
DrdI 367 GACTGTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
922 GACGAGTTTGTC
EaeI 288 TGGCCA [CT]^GGCC[GA]
1207 TGGCCA
EarI 58 GAAGAG CTCTTC(1/4)
898 GAAGAG
EcoO109I 180 AGGTCCC [GA]G^G[ACGT]CC[CT]
280 AGGACCT
HphI 203 TCACC GGTGA(8/7)
938 TCACC
Ksp632I 58 GAAGAG CTCTTC(1/4)
898 GAAGAG
MluNI 288 TGGCCA TGG^CCA
1207 TGGCCA
MscI 288 TGGCCA TGG^CCA
1207 TGGCCA
MseI 970 TTAA T^TAA
1243 TTAA
MslI 405 CATCTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
603 CACAGATGTG
PleI 118 GAGTC GAGTC(4/5)
1110 GAGTC
PpuMI 180 AGGTCCC [GA]G^G[AT]CC[CT]
280 AGGACCT
SmlI 502 CTCAAG C^T[CT][GA]AG
1050 CTTGAG
Tru9I 970 TTAA T^TAA
1243 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AvaII BanII BbvI BfaI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MaeI
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI TaqI TfiI
TseI Tsp509I TspRI TthHB8I XhoII