Restriction map for pF1KB8620
##################################
# NO CUT ENZYMES (  194 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BclI        BcnI        BfrI        BglI        
BlnI        BlpI        BmyI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       HphI        KasI        
KpnI        Ksp632I     KspI        MaeII       MamI        
MfeI        MluI        MluNI       MroI        MscI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        StyI        SwaI        Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   45 enzymes) #
###################################

AciI          486  GCGG                 CCGC(-3/-1)
AcsI           77  GAATTT               [GA]^AATT[CT]
AfaI           61  GTAC                 GT^AC
AlwI           97  GATCC                GGATC(4/5)
AlwNI         509  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI           77  GAATTT               [GA]^AATT[CT]
Asp700I       292  GAACAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvI          546  GCTGC                GCAGC(8/12)
BciVI         168  GGATAC               GTATCC(6/5)
BglII         467  AGATCT               A^GATCT
BpmI          506  CTCCAG               CTGGAG(16/14)
BsaJI         514  CCTGGG               C^C[ACGT][ACGT]GG
BsiYI         508  CCAGTGCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          508  CCAGTGCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI          424  GCATTC               GAATGC(1/-1)
Bsp1407I       60  TGTACA               T^GTACA
BsrDI         196  GCAATG               GCAATG(2/0)
BsrGI          60  TGTACA               T^GTACA
BsrI          508  CCAGT                ACTGG(1/-1)
BssKI         514  CCTGG                ^CC[ACGT]GG
BstNI         514  CCTGG                CC^[AT]GG
BstYI         467  AGATCT               [GA]^GATC[CT]
Cac8I         523  GCTCGC               GC[ACGT]^[ACGT]GC
EcoRII        514  CCTGG                ^CC[AT]GG
MaeIII        353  GTGAC                ^GT[ACGT]AC
MflI          467  AGATCT               [GA]^GATC[CT]
MslI          143  CACAACAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        544  CAGCTG               C[AC]G^C[GT]G
MvaI          514  CCTGG                CC^[AT]GG
MwoI           29  GCTCAGGAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI         508  CCAGTGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          173  CTGCAG               CTGCA^G
PvuII         544  CAGCTG               CAG^CTG
RsaI           61  GTAC                 GT^AC
ScrFI         514  CCTGG                CC^[ACGT]GG
SfaNI         139  GATGC                GCATC(5/9)
SfcI          173  CTGCAG               C^T[GA][CT]AG
SspBI          60  TGTACA               T^GTACA
TaqI          303  TCGA                 T^CGA
TseI          546  GCTGC                G^C[AT]GC
Tsp45I        353  GTGAC                ^GT[GC]AC
TthHB8I       303  TCGA                 T^CGA
Van91I        508  CCAGTGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         467  AGATCT               [GA]^GATC[CT]
XmnI          292  GAACAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AluI           14  AGCT                 AG^CT
              545  AGCT                
DpnII          97  GATC                 ^GATC
              468  GATC                
Fnu4HI        486  GCGGC                GC^[ACGT]GC
              546  GCTGC               
ItaI          486  GCGGC                GC^[ACGT]GC
              546  GCTGC               
MboI           97  GATC                 ^GATC
              468  GATC                
MnlI           35  GAGG                 CCTC(7/6)
              201  GAGG                
NdeII          97  GATC                 ^GATC
              468  GATC                
NlaIII        213  CATG                 CATG^
              539  CATG                
Sau3AI         97  GATC                 ^GATC
              468  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

BfaI        DdeI        HinfI       MaeI        MboII       
MseI        TfiI        Tru9I       Tsp509I     TspRI       
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