Restriction map for pF1KB8599
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BamHI       BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       Bsp1286I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaI        
HpaII       KasI        KpnI        KspI        MaeI        
MamI        MfeI        MflI        MluI        MroI        
MslI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SpeI        SphI        
Sse8387I    StuI        StyI        SwaI        TaqI        
TfiI        Tth111I     TthHB8I     Van91I      XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AlwI          552  GATCC                GGATC(4/5)
AvaI           15  CCCGAG               C^[CT]CG[GA]G
BalI            2  TGGCCA               TGG^CCA
BanI          220  GGCACC               G^G[CT][GA]CC
BsaAI          47  TACGTA               [CT]AC^GT[GA]
BseRI         484  CTCCTC               GAGGAG(10/8)
BsiYI         489  CCTTAACTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          489  CCTTAACTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         572  GAGAC                GTCTC(1/5)
BsmI          661  GCATTC               GAATGC(1/-1)
BsoBI          15  CCCGAG               C^[CT]CG[GA]G
Bsp1407I      650  TGTACA               T^GTACA
BsrGI         650  TGTACA               T^GTACA
BssKI         555  CCTGG                ^CC[ACGT]GG
BssSI         296  CTCGTG               CACGAG(-5/-1)
BstF5I        465  GGATG                GGATG(2/0)
BstNI         555  CCTGG                CC^[AT]GG
Cac8I           4  GCCAGC               GC[ACGT]^[ACGT]GC
DraII         593  GGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI          184  CCGTGG               C^C[GA][CT]GG
EaeI            2  TGGCCA               [CT]^GGCC[GA]
EarI          675  CTCTTC               CTCTTC(1/4)
EcoO109I      593  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRII        555  CCTGG                ^CC[AT]GG
FokI          465  GGATG                GGATG(9/13)
HaeIII          3  GGCC                 GG^CC
HincII         22  GTCAAC               GT[CT]^[GA]AC
HindII         22  GTCAAC               GT[CT]^[GA]AC
HphI           58  GGTGA                GGTGA(8/7)
Ksp632I       675  CTCTTC               CTCTTC(1/4)
MaeII          48  ACGT                 A^CGT
MboII         676  TCTTC                GAAGA(8/7)
MluNI           2  TGGCCA               TGG^CCA
MscI            2  TGGCCA               TGG^CCA
MspA1I        654  CAGCTG               C[AC]G^C[GT]G
MvaI          555  CCTGG                CC^[AT]GG
PpuMI         593  GGGACCT              [GA]G^G[AT]CC[CT]
PstI           74  CTGCAG               CTGCA^G
PvuII         654  CAGCTG               CAG^CTG
ScrFI         555  CCTGG                CC^[ACGT]GG
SnaBI          47  TACGTA               TAC^GTA
SspBI         650  TGTACA               T^GTACA
SspI          723  AATATT               AAT^ATT
Tsp45I        153  GTCAC                ^GT[GC]AC
XcmI          701  CCAGTAAAGCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AfaI           50  GTAC                 GT^AC
              651  GTAC                
AvaII         188  GGTCC                G^G[AT]CC
              594  GGACC               
BbvI           76  GCAGC                GCAGC(8/12)
              718  GCAGC               
BsaJI          16  CCGAGG               C^C[ACGT][ACGT]GG
              184  CCGTGG              
BsmFI         593  GGGAC                GGGAC(10/14)
              189  GTCCC               
Cfr13I        188  GGTCC                G^G[ACGT]CC
              594  GGACC               
DdeI          583  CTCAG                C^T[ACGT]AG
              598  CTGAG               
Fnu4HI         76  GCAGC                GC^[ACGT]GC
              718  GCAGC               
HindIII       175  AAGCTT               A^AGCTT
              546  AAGCTT              
ItaI           76  GCAGC                GC^[ACGT]GC
              718  GCAGC               
MaeIII        153  GTCAC                ^GT[ACGT]AC
              232  GTTAC               
NlaIII        217  CATG                 CATG^
              615  CATG                
RsaI           50  GTAC                 GT^AC
              651  GTAC                
Sau96I        188  GGTCC                G^G[ACGT]CC
              594  GGACC               
SfaNI         393  GCATC                GCATC(5/9)
              636  GCATC               
TseI           76  GCAGC                G^C[AT]GC
              718  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AluI        BpmI        BsrI        DpnII       HinfI       
MboI        MnlI        MseI        MwoI        NdeII       
NlaIV       PleI        Sau3AI      SfcI        Tru9I       
Tsp509I     TspRI       
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