Restriction map for pF1KB8584
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

Acc65I      AccII       AccIII      AflII       AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AvaI        
AviII       AvrII       BalI        BbeI        BbsI        
BbvCI       BcnI        BfrI        BglI        BlnI        
BpuAI       BsaBI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsoBI       BspDI       BspEI       
BspHI       BspMI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Cfr10I      ClaI        
CpoI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       FseI        FspI        
HaeII       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
SwaI        XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII        1351  GACGTC               GACGT^C
AccI          265  GTCTAC               GT^[AC][GT]AC
AclI          763  AACGTT               AA^CGTT
AcyI         1351  GACGTC               G[GA]^CG[CT]C
AflIII        770  ACATGT               A^C[GA][CT]GT
Asp700I       760  GAAAACGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI           28  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI         2143  GGCACC               G^G[CT][GA]CC
BbrPI        1769  CACGTG               CAC^GTG
BcgI          852  GCAAGACTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         819  GGATAC               GTATCC(6/5)
BclI          158  TGATCA               T^GATCA
BglII        2260  AGATCT               A^GATCT
BlpI         1066  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1066  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1769  CACGTG               [CT]AC^GT[GA]
BsaHI        1351  GACGTC               G[GA]^CG[CT]C
BsaI         2050  GAGACC               GGTCTC(1/5)
BsmFI        2202  GGGAC                GGGAC(10/14)
Bsp1407I      887  TGTACA               T^GTACA
BspLU11I      770  ACATGT               A^CATGT
BsrBI        1633  GAGCGG               CCGCTC(-3/-3)
BsrGI         887  TGTACA               T^GTACA
CelII        1066  GCTCAGC              GC^T[ACGT]AGC
CfoI         1058  GCGC                 GCG^C
DraI          333  TTTAAA               TTT^AAA
EaeI         2154  CGGCCA               [CT]^GGCC[GA]
EcoT22I      1199  ATGCAT               ATGCA^T
FbaI          158  TGATCA               T^GATCA
HapII        2070  CCGG                 C^CGG
HgaI          108  GACGC                GACGC(5/10)
HhaI         1058  GCGC                 GCG^C
Hin1I        1351  GACGTC               G[GA]^CG[CT]C
HinP1I       1058  GCGC                 G^CGC
HpaII        2070  CCGG                 C^CGG
MspI         2070  CCGG                 C^CGG
NdeI         1097  CATATG               CA^TATG
NsiI         1199  ATGCAT               ATGCA^T
PflFI          28  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1113  CCAGGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1769  CACGTG               CAC^GTG
PmlI         1769  CACGTG               CAC^GTG
Psp1406I      763  AACGTT               AA^CGTT
SacI          622  GAGCTC               GAGCT^C
SmlI          635  CTCAAG               C^T[CT][GA]AG
SspBI         887  TGTACA               T^GTACA
StuI         1005  AGGCCT               AGG^CCT
Tth111I        28  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1113  CCAGGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         2036  CCATAAGCACCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          760  GAAAACGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

ApaI          951  GGGCCC               GGGCC^C
             1939  GGGCCC              
BamHI        1014  GGATCC               G^GATCC
             1023  GGATCC              
BfaI          656  CTAG                 C^TAG
             1150  CTAG                
BseRI        1675  GAGGAG               GAGGAG(10/8)
             1678  GAGGAG              
BsmAI          26  GAGAC                GTCTC(1/5)
             2050  GAGAC               
EcoT14I      1346  CCAAGG               C^C[AT][AT]GG
             2226  CCAAGG              
FauI          682  CCCGC                CCCGC(4/6)
             1488  GCGGG               
MaeI          656  CTAG                 C^TAG
             1150  CTAG                
NspI          475  GCATGT               [GA]CATG^[CT]
              770  ACATGT              
PpuMI         968  AGGACCC              [GA]G^G[AT]CC[CT]
             2202  GGGACCT             
StyI         1346  CCAAGG               C^C[AT][AT]GG
             2226  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApoI        AspHI       AvaII       
BanII       BbvI        BmyI        BpmI        BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        BsmI        
Bsp1286I    BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Bsu36I      Cac8I       Cfr13I      
DdeI        DpnII       DraII       EarI        Eco81I      
EcoO109I    EcoRII      Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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