Restriction map for pF1KB8584
 (Linear) (Six-base) MAPSORT of: KIBB8584.seq  Check: 2175  from: 1  to: 2319



KIBB8584.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AatII G_ACGT'C

Cuts at:      0    1355    2319 
   Size:       1355     964
 
AccI GT'mk_AC

Cuts at:      0     266    2319 
   Size:        266    2053
 
AclI AA'CG_TT

Cuts at:      0     764    2319 
   Size:        764    1555
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1210    1393    1486    1735    2009    2319 
   Size:       1210     183      93     249     274     310

  Fragments arranged by size: 

                1210     310     274     249     183      93
 
AflIII A'CryG_T

Cuts at:      0     770    2319 
   Size:        770    1549
 
AleI CACnn'nnGTG

Cuts at:      0     458    2319 
   Size:        458    1861
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     898     930    2319 
   Size:        898      32    1389

  Fragments arranged by size: 

                1389     898      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     540    1119    1443    1698    1713    2319 
   Size:        540     579     324     255      15     606

  Fragments arranged by size: 

                 606     579     540     324     255      15
 
ApaI G_GGCC'C

Cuts at:      0     955    1943    2319 
   Size:        955     988     376

  Fragments arranged by size: 

                 988     955     376
 
ApoI r'AATT_y

Cuts at:      0     286     319     385    2319 
   Size:        286      33      66    1934

  Fragments arranged by size: 

                1934     286      66      33
 
BamHI G'GATC_C

Cuts at:      0    1014    1023    2319 
   Size:       1014       9    1296

  Fragments arranged by size: 

                1296    1014       9
 
BanI G'GyrC_C

Cuts at:      0    2143    2319 
   Size:       2143     176
 
BanII G_rGCy'C

Cuts at:      0     626     955    1943    2319 
   Size:        626     329     988     376

  Fragments arranged by size: 

                 988     626     376     329
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     841     875    2319 
   Size:        841      34    1444

  Fragments arranged by size: 

                1444     841      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     813    2319 
   Size:        813    1506
 
BclI T'GATC_A

Cuts at:      0     158    2319 
   Size:        158    2161
 
BfrBI ATG'CAT

Cuts at:      0    1201    2319 
   Size:       1201    1118
 
BglII A'GATC_T

Cuts at:      0    2260    2319 
   Size:       2260      59
 
BlpI GC'TnA_GC

Cuts at:      0    1067    2319 
   Size:       1067    1252
 
Bme1580I G_kGCm'C

Cuts at:      0     955    1292    1787    1943    2146    2319 
   Size:        955     337     495     156     203     173

  Fragments arranged by size: 

                 955     495     337     203     173     156
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     895     976    1409    2319 
   Size:        895      81     433     910

  Fragments arranged by size: 

                 910     895     433      81
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     786     821     931    2068    2319 
   Size:        786      35     110    1137     251

  Fragments arranged by size: 

                1137     786     251     110      35
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     620    2319 
   Size:        620    1699
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2044    2319 
   Size:       2044     275
 
BsaAI yAC'GTr

Cuts at:      0    1771    2319 
   Size:       1771     548
 
BsaHI Gr'CG_yC

Cuts at:      0    1352    2319 
   Size:       1352     967
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1690    1693    2319 
   Size:       1690       3     626

  Fragments arranged by size: 

                1690     626       3
 
BseYI C'CCAG_C

Cuts at:      0    1315    2319 
   Size:       1315    1004
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1468    1546    1768    2134    2319 
   Size:       1468      78     222     366     185

  Fragments arranged by size: 

                1468     366     222     185      78
 
BsiHKAI G_wGCw'C

Cuts at:      0     148     626    1068    2319 
   Size:        148     478     442    1251

  Fragments arranged by size: 

                1251     478     442     148
 
BsmI GAATG_Cn'

Cuts at:      0     521    1203    2003    2319 
   Size:        521     682     800     316

  Fragments arranged by size: 

                 800     682     521     316
 
Bsp1286I G_dGCh'C

Cuts at:      0     148     626     955    1068    1292    1787    1943    2146 
   Size:        148     478     329     113     224     495     156     203

Cuts at:   2146    2319 
   Size:        173

  Fragments arranged by size: 

                 495     478     329     224     203     173     156     148
                 113
 
BsrBI CCG'CTC

Cuts at:      0    1635    2319 
   Size:       1635     684
 
BsrGI T'GTAC_A

Cuts at:      0     887    2319 
   Size:        887    1432
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1446    1575    1698    2319 
   Size:       1446     129     123     621

  Fragments arranged by size: 

                1446     621     129     123
 
BstYI r'GATC_y

Cuts at:      0    1014    1023    2260    2319 
   Size:       1014       9    1237      59

  Fragments arranged by size: 

                1237    1014      59       9
 
Bsu36I CC'TnA_GG

Cuts at:      0     485     872    1925    2319 
   Size:        485     387    1053     394

  Fragments arranged by size: 

                1053     485     394     387
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2147    2319 
   Size:       2147     172
 
BtsI GCAGTG_nn'

Cuts at:      0    1292    2319 
   Size:       1292    1027
 
DraI TTT'AAA

Cuts at:      0     335    2319 
   Size:        335    1984
 
EaeI y'GGCC_r

Cuts at:      0    2154    2319 
   Size:       2154     165
 
EarI CTCTTCn'nnn_

Cuts at:      0      18     189     194     245    1148    2319 
   Size:         18     171       5      51     903    1171

  Fragments arranged by size: 

                1171     903     171      51      18       5
 
EcoICRI GAG'CTC

Cuts at:      0     624    2319 
   Size:        624    1695
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     786     821     931    1210    1393    1486    1735    2009 
   Size:        786      35     110     279     183      93     249     274

Cuts at:   2009    2068    2319 
   Size:         59     251

  Fragments arranged by size: 

                 786     279     274     251     249     183     110      93
                  59      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0     951     969    1939    1940    2203    2230    2319 
   Size:        951      18     970       1     263      27      89

  Fragments arranged by size: 

                 970     951     263      89      27      18       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     601    2319 
   Size:        601    1718
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      17      49     332     364    1203    1235    1258    1290 
   Size:         17      32     283      32     839      32      23      32

Cuts at:   1290    2319 
   Size:       1029

  Fragments arranged by size: 

                1029     839     283      32      32      32      32      23
                  17
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     974    1406    2319 
   Size:        974     432     913

  Fragments arranged by size: 

                 974     913     432
 
MslI CAynn'nnrTG

Cuts at:      0     166     458     658    2319 
   Size:        166     292     200    1661

  Fragments arranged by size: 

                1661     292     200     166
 
MspA1I CmG'CkG

Cuts at:      0     208     685    1479    1485    1497    1506    1698    1849 
   Size:        208     477     794       6      12       9     192     151

Cuts at:   1849    1956    2319 
   Size:        107     363

  Fragments arranged by size: 

                 794     477     363     208     192     151     107      12
                   9       6
 
NdeI CA'TA_TG

Cuts at:      0    1098    2319 
   Size:       1098    1221
 
NsiI A_TGCA'T

Cuts at:      0    1203    2319 
   Size:       1203    1116
 
NspI r_CATG'y

Cuts at:      0     479     774    2319 
   Size:        479     295    1545

  Fragments arranged by size: 

                1545     479     295
 
PciI A'CATG_T

Cuts at:      0     770    2319 
   Size:        770    1549
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1119    2319 
   Size:       1119    1200
 
PfoI T'CCnGG_A

Cuts at:      0     815    2319 
   Size:        815    1504
 
PmlI CAC'GTG

Cuts at:      0    1771    2319 
   Size:       1771     548
 
PpuMI rG'GwC_Cy

Cuts at:      0     969    2203    2319 
   Size:        969    1234     116

  Fragments arranged by size: 

                1234     969     116
 
PspOMI G'GGCC_C

Cuts at:      0     951    1939    2319 
   Size:        951     988     380

  Fragments arranged by size: 

                 988     951     380
 
PstI C_TGCA'G

Cuts at:      0     545    1565    1598    2292    2319 
   Size:        545    1020      33     694      27

  Fragments arranged by size: 

                1020     694     545      33      27
 
PvuII CAG'CTG

Cuts at:      0     208    1485    1497    1506    1698    1849    1956    2319 
   Size:        208    1277      12       9     192     151     107     363

  Fragments arranged by size: 

                1277     363     208     192     151     107      12       9
            
 
SacI G_AGCT'C

Cuts at:      0     626    2319 
   Size:        626    1693
 
SfcI C'TryA_G

Cuts at:      0     112     541    1561    1594    1627    1660    2288    2319 
   Size:        112     429    1020      33      33      33     628      31

  Fragments arranged by size: 

                1020     628     429     112      33      33      33      31
            
 
SmlI C'TyrA_G

Cuts at:      0     635    2319 
   Size:        635    1684
 
StuI AGG'CCT

Cuts at:      0    1007    2319 
   Size:       1007    1312
 
StyI C'CwwG_G

Cuts at:      0    1346    2226    2319 
   Size:       1346     880      93

  Fragments arranged by size: 

                1346     880      93
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2089    2319 
   Size:       2089     230
 
TatI w'GTAC_w

Cuts at:      0     582     805     887    2319 
   Size:        582     223      82    1432

  Fragments arranged by size: 

                1432     582     223      82
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1342    1374    2207    2239    2319 
   Size:       1342      32     833      32      80

  Fragments arranged by size: 

                1342     833      80      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      31    2319 
   Size:         31    2288
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2043    2319 
   Size:       2043     276
 
XmnI GAAnn'nnTTC

Cuts at:      0     764    2319 
   Size:        764    1555
 
ZraI GAC'GTC

Cuts at:      0    1353    2319 
   Size:       1353     966
 

 Enzymes that do cut:

    AatII     AccI     AclI     AcuI   AflIII     AleI     AloI    AlwNI
     ApaI     ApoI    BamHI     BanI    BanII     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI Bme1580I     BmrI     BpmI    BpuEI     BsaI
    BsaAI    BsaHI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BsrBI    BsrGI   BstAPI    BstYI   Bsu36I    BtgZI     BtsI     DraI
     EaeI     EarI  EcoICRI  Eco57MI EcoO109I     FalI    Hin4I     MmeI
     MslI   MspA1I     NdeI     NsiI     NspI     PciI    PflMI     PfoI
     PmlI    PpuMI   PspOMI     PstI    PvuII     SacI     SfcI     SmlI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AfeI    AflII     AgeI     AhdI     AlfI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI     BbsI
    BbvCI     BglI    BmgBI     BmtI     BplI   Bpu10I    BsaBI    BsaWI
    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrDI
    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I     BtgI
     ClaI    CspCI   DraIII     DrdI     EagI     EciI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PmeI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     XbaI     XhoI     XmaI
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