Restriction map for pF1KB8577
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspEI       AvaI        AvrII       BalI        BanI        
BbeI        BbrPI       BbvCI       BcnI        BfrI        
BglI        BlnI        BsaAI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsoBI       Bsp1407I    
BspEI       BspHI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstEII      BstPI       BstUI       
BstXI       BstZ17I     Cfr10I      CpoI        DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     FauI        FseI        
HaeII       HapII       HgaI        HindIII     HpaII       
KasI        KpnI        Ksp632I     KspI        MluI        
MluNI       MroI        MscI        MspI        MvnI        
NaeI        NarI        NciI        NgoMIV      NheI        
NotI        NruI        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SmlI        
SnaBI       SphI        Sse8387I    SspBI       Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AatII          43  GACGTC               GACGT^C
AcyI           43  GACGTC               G[GA]^CG[CT]C
AseI         2494  ATTAAT               AT^TAAT
AsnI         2494  ATTAAT               AT^TAAT
Asp700I       601  GAAGTAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         2434  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         857  TGCGCA               TGC^GCA
BamHI        1502  GGATCC               G^GATCC
BanII        1661  GAGCTC               G[GA]GC[CT]^C
BbsI         2467  GAAGAC               GAAGAC(2/6)
BcgI         1066  GCAATTAAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         2378  TGATCA               T^GATCA
BglII        1674  AGATCT               A^GATCT
BlpI         1284  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1284  GCTCAGC              GC^T[ACGT]AGC
BpuAI        2467  GAAGAC               GAAGAC(2/6)
BsaBI         544  GATATTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          43  GACGTC               G[GA]^CG[CT]C
BsaI         1457  GAGACC               GGTCTC(1/5)
BseRI         580  GAGGAG               GAGGAG(10/8)
BspDI         559  ATCGAT               AT^CGAT
BsrBI        1023  CCGCTC               CCGCTC(-3/-3)
BstAPI        942  GCATAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         608  TTCGAA               TT^CGAA
CelII        1284  GCTCAGC              GC^T[ACGT]AGC
CfoI          858  GCGC                 GCG^C
ClaI          559  ATCGAT               AT^CGAT
DraI          657  TTTAAA               TTT^AAA
DraIII       1178  CACGATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRI          26  GAATTC               G^AATTC
EcoRV          37  GATATC               GAT^ATC
FbaI         2378  TGATCA               T^GATCA
FspI          857  TGCGCA               TGC^GCA
HhaI          858  GCGC                 GCG^C
Hin1I          43  GACGTC               G[GA]^CG[CT]C
HinP1I        858  GCGC                 G^CGC
HincII       1969  GTTAAC               GT[CT]^[GA]AC
HindII       1969  GTTAAC               GT[CT]^[GA]AC
HpaI         1969  GTTAAC               GTT^AAC
MamI          544  GATATTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI         2166  CCATGG               C^CATGG
NdeI          191  CATATG               CA^TATG
NspV          608  TTCGAA               TT^CGAA
PflFI        2434  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        2494  ATTAAT               AT^TAAT
PstI         1103  CTGCAG               CTGCA^G
SacI         1661  GAGCTC               GAGCT^C
SfaNI        2485  GATGC                GCATC(5/9)
SfuI          608  TTCGAA               TT^CGAA
SpeI         1680  ACTAGT               A^CTAGT
StuI         1573  AGGCCT               AGG^CCT
SwaI          656  ATTTAAAT             ATTT^AAAT
Tth111I      2434  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI          601  GAAGTAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcsI           26  GAATTC               [GA]^AATT[CT]
              403  GAATTT              
AflIII        162  ACATGT               A^C[GA][CT]GT
             1545  ACATGT              
AocI         1373  CCTCAGG              CC^T[ACGT]AGG
             2121  CCTGAGG             
ApoI           26  GAATTC               [GA]^AATT[CT]
              403  GAATTT              
AspHI        1186  GAGCAC               G[AT]GC[AT]^C
             1661  GAGCTC              
AvaII        1013  GGACC                G^G[AT]CC
             1899  GGACC               
BsiHKAI      1186  GAGCAC               G[AT]GC[AT]^C
             1661  GAGCTC              
BsmAI        1457  GAGAC                GTCTC(1/5)
              831  GTCTC               
BsmFI         720  GGGAC                GGGAC(10/14)
             2422  GTCCC               
BspLU11I      162  ACATGT               A^CATGT
             1545  ACATGT              
BsrDI        1923  GCAATG               GCAATG(2/0)
             2173  GCAATG              
Bsu36I       1373  CCTCAGG              CC^T[ACGT]AGG
             2121  CCTGAGG             
DraII        1012  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1898  AGGACCC             
DsaI         1461  CCGTGG               C^C[GA][CT]GG
             2166  CCATGG              
EaeI         1020  TGGCCG               [CT]^GGCC[GA]
             2472  CGGCCA              
Eco81I       1373  CCTCAGG              CC^T[ACGT]AGG
             2121  CCTGAGG             
EcoO109I     1012  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1898  AGGACCC             
EcoT14I      2166  CCATGG               C^C[AT][AT]GG
             2261  CCAAGG              
MaeII          44  ACGT                 A^CGT
              128  ACGT                
PleI          106  GACTC                GAGTC(4/5)
             1155  GACTC               
PpuMI        1012  AGGACCT              [GA]G^G[AT]CC[CT]
             1898  AGGACCC             
PvuII         727  CAGCTG               CAG^CTG
             1106  CAGCTG              
SfcI          233  CTACAG               C^T[GA][CT]AG
             1103  CTGCAG              
SspI         1520  AATATT               AAT^ATT
             2491  AATATT              
StyI         2166  CCATGG               C^C[AT][AT]GG
             2261  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BciVI       BfaI        BmyI        BpmI        BsaJI       
BsgI        BsiYI       BslI        BsmI        Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
EcoRII      EcoT22I     Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MfeI        MflI        MnlI        
MseI        MslI        MspA1I      MunI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NsiI        
NspI        RsaI        Sau3AI      Sau96I      ScrFI       
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com