Restriction map for pF1KB8577
 (Linear) (Six-base) MAPSORT of: KIBB8577.seq  Check: 1071  from: 1  to: 2562



KIBB8577.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AatII G_ACGT'C

Cuts at:      0      47    2562 
   Size:         47    2515
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     401    2562 
   Size:        191     210    2161

  Fragments arranged by size: 

                2161     210     191
 
AflIII A'CryG_T

Cuts at:      0     162    1545    2562 
   Size:        162    1383    1017

  Fragments arranged by size: 

                1383    1017     162
 
AleI CACnn'nnGTG

Cuts at:      0     762    2562 
   Size:        762    1800
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     948     980    1487    1519    2562 
   Size:        948      32     507      32    1043

  Fragments arranged by size: 

                1043     948     507      32      32
 
ApoI r'AATT_y

Cuts at:      0      26     403    2562 
   Size:         26     377    2159

  Fragments arranged by size: 

                2159     377      26
 
AseI AT'TA_AT

Cuts at:      0    2495    2562 
   Size:       2495      67
 
BamHI G'GATC_C

Cuts at:      0    1502    2562 
   Size:       1502    1060
 
BanII G_rGCy'C

Cuts at:      0    1665    2562 
   Size:       1665     897
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2474    2562 
   Size:       2474      88
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1055    1089    2562 
   Size:       1055      34    1473

  Fragments arranged by size: 

                1473    1055      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     159    1399    1695    2562 
   Size:        159    1240     296     867

  Fragments arranged by size: 

                1240     867     296     159
 
BclI T'GATC_A

Cuts at:      0    2378    2562 
   Size:       2378     184
 
BfrBI ATG'CAT

Cuts at:      0    1445    1992    2360    2562 
   Size:       1445     547     368     202

  Fragments arranged by size: 

                1445     547     368     202
 
BglII A'GATC_T

Cuts at:      0    1674    2562 
   Size:       1674     888
 
BlpI GC'TnA_GC

Cuts at:      0    1285    2562 
   Size:       1285    1277
 
Bme1580I G_kGCm'C

Cuts at:      0     674    1308    2562 
   Size:        674     634    1254

  Fragments arranged by size: 

                1254     674     634
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     661    2562 
   Size:        661    1901
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1382    2562 
   Size:       1382    1180
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1190    1284    1679    2562 
   Size:       1190      94     395     883

  Fragments arranged by size: 

                1190     883     395      94
 
Bpu10I CC'TnA_GC

Cuts at:      0    1570    1664    2562 
   Size:       1570      94     898

  Fragments arranged by size: 

                1570     898      94
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1451    2562 
   Size:       1451    1111
 
BsaBI GATnn'nnATC

Cuts at:      0     548    2562 
   Size:        548    2014
 
BsaHI Gr'CG_yC

Cuts at:      0      44    2562 
   Size:         44    2518
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      91     121     407     437     916     946     948     978 
   Size:         91      30     286      30     479      30       2      30

Cuts at:    978    1652    1682    2098    2128    2562 
   Size:        674      30     416      30     434

  Fragments arranged by size: 

                 674     479     434     416     286      91      30      30
                  30      30      30      30       2
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     595    2562 
   Size:        595    1967
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     781    1495    2380    2427    2562 
   Size:        427     354     714     885      47     135

  Fragments arranged by size: 

                 885     714     427     354     135      47
 
BsiHKAI G_wGCw'C

Cuts at:      0    1190    1665    2562 
   Size:       1190     475     897

  Fragments arranged by size: 

                1190     897     475
 
BsmI GAATG_Cn'

Cuts at:      0    1129    1445    2513    2562 
   Size:       1129     316    1068      49

  Fragments arranged by size: 

                1129    1068     316      49
 
Bsp1286I G_dGCh'C

Cuts at:      0     674    1190    1308    1665    2562 
   Size:        674     516     118     357     897

  Fragments arranged by size: 

                 897     674     516     357     118
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     851    1214    2402    2562 
   Size:        851     363    1188     160

  Fragments arranged by size: 

                1188     851     363     160
 
BsrBI CCG'CTC

Cuts at:      0    1025    2562 
   Size:       1025    1537
 
BsrDI GCAATG_nn'

Cuts at:      0    1930    2180    2562 
   Size:       1930     250     382

  Fragments arranged by size: 

                1930     382     250
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     948    2562 
   Size:        948    1614
 
BstBI TT'CG_AA

Cuts at:      0     609    2562 
   Size:        609    1953
 
BstYI r'GATC_y

Cuts at:      0    1173    1502    1674    1755    2525    2562 
   Size:       1173     329     172      81     770      37

  Fragments arranged by size: 

                1173     770     329     172      81      37
 
Bsu36I CC'TnA_GG

Cuts at:      0    1374    2122    2562 
   Size:       1374     748     440

  Fragments arranged by size: 

                1374     748     440
 
BtgI C'CryG_G

Cuts at:      0    1461    2166    2562 
   Size:       1461     705     396

  Fragments arranged by size: 

                1461     705     396
 
BtsI GCAGTG_nn'

Cuts at:      0     769    2562 
   Size:        769    1793
 
ClaI AT'CG_AT

Cuts at:      0     560    2562 
   Size:        560    2002
 
DraI TTT'AAA

Cuts at:      0     659    2562 
   Size:        659    1903
 
DraIII CAC_nnn'GTG

Cuts at:      0    1183    2562 
   Size:       1183    1379
 
EaeI y'GGCC_r

Cuts at:      0    1020    2472    2562 
   Size:       1020    1452      90

  Fragments arranged by size: 

                1452    1020      90
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1063    2562 
   Size:       1063    1499
 
EcoICRI GAG'CTC

Cuts at:      0    1663    2562 
   Size:       1663     899
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     401    1190    1284    1679    2562 
   Size:        191     210     789      94     395     883

  Fragments arranged by size: 

                 883     789     395     210     191      94
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1013    1899    2562 
   Size:       1013     886     663

  Fragments arranged by size: 

                1013     886     663
 
EcoRI G'AATT_C

Cuts at:      0      26    2562 
   Size:         26    2536
 
EcoRV GAT'ATC

Cuts at:      0      39    2562 
   Size:         39    2523
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1244    2562 
   Size:       1244    1318
 
FspI TGC'GCA

Cuts at:      0     859    2562 
   Size:        859    1703
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     916     948    2115    2147    2179    2562 
   Size:        916      32    1167      32      32     383

  Fragments arranged by size: 

                1167     916     383      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1971    2562 
   Size:       1971     591
 
HpaI GTT'AAC

Cuts at:      0    1971    2562 
   Size:       1971     591
 
MfeI C'AATT_G

Cuts at:      0     789    1481    1978    2562 
   Size:        789     692     497     584

  Fragments arranged by size: 

                 789     692     584     497
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1685    2562 
   Size:       1685     877
 
MslI CAynn'nnrTG

Cuts at:      0     560     762    2402    2562 
   Size:        560     202    1640     160

  Fragments arranged by size: 

                1640     560     202     160
 
MspA1I CmG'CkG

Cuts at:      0     729    1108    1500    2562 
   Size:        729     379     392    1062

  Fragments arranged by size: 

                1062     729     392     379
 
NcoI C'CATG_G

Cuts at:      0    2166    2562 
   Size:       2166     396
 
NdeI CA'TA_TG

Cuts at:      0     192    2562 
   Size:        192    2370
 
NsiI A_TGCA'T

Cuts at:      0    1447    1994    2362    2562 
   Size:       1447     547     368     200

  Fragments arranged by size: 

                1447     547     368     200
 
NspI r_CATG'y

Cuts at:      0     166     505    1549    2562 
   Size:        166     339    1044    1013

  Fragments arranged by size: 

                1044    1013     339     166
 
PasI CC'CwG_GG

Cuts at:      0    1043    1620    2562 
   Size:       1043     577     942

  Fragments arranged by size: 

                1043     942     577
 
PciI A'CATG_T

Cuts at:      0     162    1545    2562 
   Size:        162    1383    1017

  Fragments arranged by size: 

                1383    1017     162
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     948     980    2562 
   Size:        948      32    1582

  Fragments arranged by size: 

                1582     948      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1013    1899    2562 
   Size:       1013     886     663

  Fragments arranged by size: 

                1013     886     663
 
PstI C_TGCA'G

Cuts at:      0    1107    2562 
   Size:       1107    1455
 
PvuII CAG'CTG

Cuts at:      0     729    1108    2562 
   Size:        729     379    1454

  Fragments arranged by size: 

                1454     729     379
 
SacI G_AGCT'C

Cuts at:      0    1665    2562 
   Size:       1665     897
 
SfcI C'TryA_G

Cuts at:      0     233    1103    2562 
   Size:        233     870    1459

  Fragments arranged by size: 

                1459     870     233
 
SpeI A'CTAG_T

Cuts at:      0    1680    2562 
   Size:       1680     882
 
SspI AAT'ATT

Cuts at:      0    1522    2493    2562 
   Size:       1522     971      69

  Fragments arranged by size: 

                1522     971      69
 
StuI AGG'CCT

Cuts at:      0    1575    2562 
   Size:       1575     987
 
StyI C'CwwG_G

Cuts at:      0    2166    2261    2562 
   Size:       2166      95     301

  Fragments arranged by size: 

                2166     301      95
 
SwaI ATTT'AAAT

Cuts at:      0     659    2562 
   Size:        659    1903
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     467    2562 
   Size:        467    2095
 
TatI w'GTAC_w

Cuts at:      0     159     361    2562 
   Size:        159     202    2201

  Fragments arranged by size: 

                2201     202     159
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     407     439    1652    1684    1991    2023    2562 
   Size:        407      32    1213      32     307      32     539

  Fragments arranged by size: 

                1213     539     407     307      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2437    2562 
   Size:       2437     125
 
XmnI GAAnn'nnTTC

Cuts at:      0     605    2562 
   Size:        605    1957
 
ZraI GAC'GTC

Cuts at:      0      45    2562 
   Size:         45    2517
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     AleI     AloI     ApoI     AseI    BamHI
    BanII     BbsI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaBI    BsaHI
    BsaXI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI
    BsrDI   BstAPI    BstBI    BstYI   Bsu36I     BtgI     BtsI     ClaI
     DraI   DraIII     EaeI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI     FspI    Hin4I   HincII     HpaI     MfeI     MmeI
     MslI   MspA1I     NcoI     NdeI     NsiI     NspI     PasI     PciI
     PpiI    PpuMI     PstI    PvuII     SacI     SfcI     SpeI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI     BanI     BbeI    BbvCI     BglI    BmgBI     BmtI    BpuEI
    BsaAI    BsaWI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrFI    BsrGI   BssHII    BssSI   BstEII    BstXI  BstZ17I    BtgZI
    CspCI     DrdI     EagI     EarI    EcoNI     FseI    FspAI    HaeII
  HindIII     KasI     KpnI     MluI     MscI     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     PacI    PflMI     PfoI     PmeI     PmlI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI     XbaI
     XcmI     XhoI     XmaI
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