Restriction map for pF1KB8575
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
AocI        Aor51HI     ApaI        ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BfrI        BglII       BlnI        BsaAI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspMI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FokI        FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        KspI        MaeII       
MfeI        MluI        MluNI       MroI        MscI        
MseI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI          406  GTCTAC               GT^[AC][GT]AC
AflIII        908  ACATGT               A^C[GA][CT]GT
AlwI          357  GATCC                GGATC(4/5)
Asp700I       372  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          755  CCCGGG               C^[CT]CG[GA]G
BanI          112  GGTGCC               G^G[CT][GA]CC
BbsI          567  GAAGAC               GAAGAC(2/6)
BclI          423  TGATCA               T^GATCA
BglI          664  GCCCAGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          966  GCTAAGC              GC^T[ACGT]AGC
BpmI          196  CTCCAG               CTGGAG(16/14)
Bpu1102I      966  GCTAAGC              GC^T[ACGT]AGC
BpuAI         567  GAAGAC               GAAGAC(2/6)
BsaBI         433  GATGAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          184  GTGCAG               GTGCAG(16/14)
BsmBI         997  GAGACG               CGTCTC(1/5)
BsoBI         755  CCCGGG               C^[CT]CG[GA]G
BspLU11I      908  ACATGT               A^CATGT
BsrBI         628  GAGCGG               CCGCTC(-3/-3)
BstYI         356  AGATCC               [GA]^GATC[CT]
CelII         966  GCTAAGC              GC^T[ACGT]AGC
CfoI          640  GCGC                 GCG^C
DsaI          473  CCATGG               C^C[GA][CT]GG
EaeI         1001  CGGCCA               [CT]^GGCC[GA]
FbaI          423  TGATCA               T^GATCA
HaeII         639  GGCGCT               [GA]GCGC^[CT]
HapII         756  CCGG                 C^CGG
HhaI          640  GCGC                 GCG^C
HinP1I        640  GCGC                 G^CGC
HinfI         919  GACTC                G^A[ACGT]TC
HpaII         756  CCGG                 C^CGG
MaeIII        508  GTAAC                ^GT[ACGT]AC
MamI          433  GATGAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          356  AGATCC               [GA]^GATC[CT]
MspI          756  CCGG                 C^CGG
NcoI          473  CCATGG               C^CATGG
NheI          954  GCTAGC               G^CTAGC
NspI          908  ACATGT               [GA]CATG^[CT]
PleI          919  GACTC                GAGTC(4/5)
PpuMI         461  AGGACCT              [GA]G^G[AT]CC[CT]
SmaI          755  CCCGGG               CCC^GGG
SpeI          232  ACTAGT               A^CTAGT
TaqI          127  TCGA                 T^CGA
TthHB8I       127  TCGA                 T^CGA
XhoII         356  AGATCC               [GA]^GATC[CT]
XmaCI         755  CCCGGG               C^CCGGG
XmaI          755  CCCGGG               C^CCGGG
XmnI          372  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AfaI          220  GTAC                 GT^AC
              855  GTAC                
Alw44I         67  GTGCAC               G^TGCAC
              388  GTGCAC              
AlwNI         460  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
              773  CAGATGCTG           
ApaLI          67  GTGCAC               G^TGCAC
              388  GTGCAC              
AspHI          67  GTGCAC               G[AT]GC[AT]^C
              388  GTGCAC              
AvaII         285  GGTCC                G^G[AT]CC
              462  GGACC               
BbvI          843  GCAGC                GCAGC(8/12)
              742  GCTGC               
BcnI          755  CCCGG                CC^[GC]GG
              756  CCGGG               
BfaI          233  CTAG                 C^TAG
              955  CTAG                
BsiHKAI        67  GTGCAC               G[AT]GC[AT]^C
              388  GTGCAC              
BsiYI         480  CCCTGGGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              756  CCGGGAGAAGG         
BslI          480  CCCTGGGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              756  CCGGGAGAAGG         
BsrDI         582  CATTGC               GCAATG(2/0)
              864  CATTGC              
DraII         338  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              461  AGGACCT             
EarI           55  CTCTTC               CTCTTC(1/4)
               79  GAAGAG              
EcoO109I      338  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              461  AGGACCT             
EcoT14I       473  CCATGG               C^C[AT][AT]GG
              992  CCTTGG              
FauI          504  GCGGG                CCCGC(4/6)
              925  GCGGG               
HindIII       574  AAGCTT               A^AGCTT
              969  AAGCTT              
Ksp632I        55  CTCTTC               CTCTTC(1/4)
               79  GAAGAG              
MaeI          233  CTAG                 C^TAG
              955  CTAG                
MslI          789  CACTGCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              909  CATGTTCATG          
NciI          755  CCCGG                CC^[GC]GG
              756  CCGGG               
PstI          381  CTGCAG               CTGCA^G
              841  CTGCAG              
PvuII          62  CAGCTG               CAG^CTG
              844  CAGCTG              
RsaI          220  GTAC                 GT^AC
              855  GTAC                
SfcI          381  CTGCAG               C^T[GA][CT]AG
              841  CTGCAG              
StyI          473  CCATGG               C^C[AT][AT]GG
              992  CCTTGG              
TseI          742  GCTGC                G^C[AT]GC
              843  GCAGC               
Tsp509I       264  AATT                 ^AATT
              732  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AciI        AluI        BmyI        BsaJI       BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      HaeIII      HphI        ItaI        MboI        
MboII       MnlI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       SfaNI       TspRI       
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