Restriction map for pF1KB8575
 (Circular) (Six-base) MAPSORT of: pF1KB8575.seq  Check: 446  from: 1  to: 4130



pF1KB8575  4130 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:29  ..

AccI GT'mk_AC

Cuts at:    515    1168     515 
   Size:        653    3477
 
Acc65I G'GTAC_C

Cuts at:   1146    1146 
   Size:       4130
 
AclI AA'CG_TT

Cuts at:   3970    3970 
   Size:       4130
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    949    1955    2387    2740    3402     949 
   Size:       1006     432     353     662    1677

  Fragments arranged by size: 

                1677    1006     662     432     353
 
AflIII A'CryG_T

Cuts at:   1016    1482    3282    3563    1016 
   Size:        466    1800     281    1583

  Fragments arranged by size: 

                1800    1583     466     281
 
AlwNI CAG_nnn'CTG

Cuts at:    573     886    1435    2873     573 
   Size:        313     549    1438    1830

  Fragments arranged by size: 

                1830    1438     549     313
 
ApaLI G'TGCA_C

Cuts at:    175     496    2968     175 
   Size:        321    2472    1337

  Fragments arranged by size: 

                2472    1337     321
 
ApoI r'AATT_y

Cuts at:   1134    3483    3739    1134 
   Size:       2349     256    1525

  Fragments arranged by size: 

                2349    1525     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4130
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4130
 
AvaI C'yCGr_G

Cuts at:    863    1150     863 
   Size:        287    3843
 
BamHI G'GATC_C

Cuts at:   1155    1155 
   Size:       4130
 
BanI G'GyrC_C

Cuts at:    220    1146    1560    1808    1843     220 
   Size:        926     414     248      35    2507

  Fragments arranged by size: 

                2507     926     414     248      35
 
BanII G_rGCy'C

Cuts at:   1144    2174    1144 
   Size:       1030    3100
 
BbeI G_GCGC'C

Cuts at:   1564    1812    1564 
   Size:        248    3882
 
BbsI GAAGACnn'nnnn_

Cuts at:    682     682 
   Size:       4130
 
BciVI GTATCCnnnnn_n'

Cuts at:   2028    3084    4130    2028 
   Size:       1056    1046    2028

  Fragments arranged by size: 

                2028    1056    1046
 
BclI T'GATC_A

Cuts at:    531    1650     531 
   Size:       1119    3011
 
BglI GCCn_nnn'nGGC

Cuts at:    778     778 
   Size:       4130
 
BglII A'GATC_T

Cuts at:   1645    3682    1645 
   Size:       2037    2093
 
BlpI GC'TnA_GC

Cuts at:   1075    1242    1075 
   Size:        167    3963
 
Bme1580I G_kGCm'C

Cuts at:    179     225     500    1755    1848    2972     179 
   Size:         46     275    1255      93    1124    1337

  Fragments arranged by size: 

                1337    1255    1124     275      93      46
 
BmrI ACTGGGnnnn_n'

Cuts at:   1342    1423    1758    3902    1342 
   Size:         81     335    2144    1570

  Fragments arranged by size: 

                2144    1570     335      81
 
BmtI G_CTAG'C

Cuts at:   1066    1066 
   Size:       4130
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2497    2497 
   Size:       4130
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    289     289 
   Size:       4130
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1306    2185    2899    3197    1306 
   Size:        879     714     298    2239

  Fragments arranged by size: 

                2239     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3834    3834 
   Size:       4130
 
BsaAI yAC'GTr

Cuts at:   1483    1483 
   Size:       4130
 
BsaBI GATnn'nnATC

Cuts at:      6     545    1644    3681       6 
   Size:        539    1099    2037     455

  Fragments arranged by size: 

                2037    1099     539     455
 
BsaHI Gr'CG_yC

Cuts at:   1561    1809    2511    4007    1561 
   Size:        248     702    1496    1684

  Fragments arranged by size: 

                1684    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1319    1840    2518    2929    3076    3350    1319 
   Size:        521     678     411     147     274    2099

  Fragments arranged by size: 

                2099     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    113     143     562     592     113 
   Size:         30     419      30    3651

  Fragments arranged by size: 

                3651     419      30      30
 
BseYI C'CCAG_C

Cuts at:   1555    2313    2978    3386    1555 
   Size:        758     665     408    2299

  Fragments arranged by size: 

                2299     758     665     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    313     313 
   Size:       4130
 
BsiEI CG_ry'CG

Cuts at:    104    1672    1718    2948     104 
   Size:       1568      46    1230    1286

  Fragments arranged by size: 

                1568    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    179     500    1144    1922    2112    2972     179 
   Size:        321     644     778     190     860    1337

  Fragments arranged by size: 

                1337     860     778     644     321     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1099    1099 
   Size:       4130
 
Bsp1286I G_dGCh'C

Cuts at:    179     225     500    1144    1755    1848    1922    2112    2174 
   Size:         46     275     644     611      93      74     190      62

Cuts at:   2174    2972     179 
   Size:        798    1337

  Fragments arranged by size: 

                1337     798     644     611     275     190      93      74
                  62      46
 
BspHI T'CATG_A

Cuts at:   2562    2562 
   Size:       4130
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1180    1394    1696    2077    1180 
   Size:        214     302     381    3233

  Fragments arranged by size: 

                3233     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     738    2422    2476    3960    4125      50 
   Size:        688    1684      54    1484     165      55

  Fragments arranged by size: 

                1684    1484     688     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    689     971    2042     689 
   Size:        282    1071    2777

  Fragments arranged by size: 

                2777    1071     282
 
BsrFI r'CCGG_y

Cuts at:   2128    2309    2128 
   Size:        181    3949
 
BssSI C'ACGA_G

Cuts at:   2401    3109    2401 
   Size:        708    3422
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1507    1507 
   Size:       4130
 
BstBI TT'CG_AA

Cuts at:   2491    2491 
   Size:       4130
 
BstYI r'GATC_y

Cuts at:      1     464    1155    1622    1645    1980    2226    2532    2544 
   Size:        463     691     467      23     335     246     306      12

Cuts at:   2544    2630    3682       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                1052     691     467     463     449     335     306     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     581     107 
   Size:        474    3656
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2080    2261    2424    2080 
   Size:        181     163    3786

  Fragments arranged by size: 

                3786     181     163
 
BtsI GCAGTG_nn'

Cuts at:    410     498     896    1044    1373     410 
   Size:         88     398     148     329    3167

  Fragments arranged by size: 

                3167     398     329     148      88
 
DraI TTT'AAA

Cuts at:   1129    1129 
   Size:       4130
 
DrdI GACnn_nn'nnGTC

Cuts at:   1836    3180    1836 
   Size:       1344    2786
 
EaeI y'GGCC_r

Cuts at:   1109    1715    1889    2280    2307    3652    1109 
   Size:        606     174     391      27    1345    1587

  Fragments arranged by size: 

                1587    1345     606     391     174      27
 
EagI C'GGCC_G

Cuts at:   1715    1715 
   Size:       4130
 
EarI CTCTTCn'nnn_

Cuts at:    169     182    2153    2363     169 
   Size:         13    1971     210    1936

  Fragments arranged by size: 

                1971    1936     210      13
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2338    3080    3226    3940    2338 
   Size:        742     146     714    2528

  Fragments arranged by size: 

                2528     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1142    1142 
   Size:       4130
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    289     949    1955    2387    2740    3402     289 
   Size:        660    1006     432     353     662    1017

  Fragments arranged by size: 

                1017    1006     662     660     432     353
 
EcoO109I rG'GnC_Cy

Cuts at:    447     570    1269     447 
   Size:        123     699    3308

  Fragments arranged by size: 

                3308     699     123
 
EcoRI G'AATT_C

Cuts at:   1134    1134 
   Size:       4130
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1011    1095    2149    1011 
   Size:         84    1054    2992

  Fragments arranged by size: 

                2992    1054      84
 
FspI TGC'GCA

Cuts at:   1911    1911 
   Size:       4130
 
HaeII r_GCGC'y

Cuts at:    751    1564    1812    3042    3813     751 
   Size:        813     248    1230     771    1068

  Fragments arranged by size: 

                1230    1068     813     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    562     594    1972    2002    2004    2034     562 
   Size:         32    1378      30       2      30    2658

  Fragments arranged by size: 

                2658    1378      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1169    1169 
   Size:       4130
 
HindIII A'AGCT_T

Cuts at:    682    1077     682 
   Size:        395    3735
 
KasI G'GCGC_C

Cuts at:   1560    1808    1560 
   Size:        248    3882
 
KpnI G_GTAC'C

Cuts at:   1150    1150 
   Size:       4130
 
MluI A'CGCG_T

Cuts at:   3563    3563 
   Size:       4130
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1004    2889    3073    1004 
   Size:       1885     184    2061

  Fragments arranged by size: 

                2061    1885     184
 
MscI TGG'CCA

Cuts at:   1891    1891 
   Size:       4130
 
MslI CAynn'nnrTG

Cuts at:    901    1021    2119    2246     901 
   Size:        120    1098     127    2785

  Fragments arranged by size: 

                2785    1098     127     120
 
MspA1I CmG'CkG

Cuts at:    172     242     954    1241    1555    1915    2699    2944     172 
   Size:         70     712     287     314     360     784     245    1358

  Fragments arranged by size: 

                1358     784     712     360     314     287     245      70
            
 
NaeI GCC'GGC

Cuts at:   2311    2311 
   Size:       4130
 
NarI GG'CG_CC

Cuts at:   1561    1809    1561 
   Size:        248    3882
 
NcoI C'CATG_G

Cuts at:    107     581     107 
   Size:        474    3656
 
NgoMIV G'CCGG_C

Cuts at:   2309    2309 
   Size:       4130
 
NheI G'CTAG_C

Cuts at:   1062    1062 
   Size:       4130
 
NspI r_CATG'y

Cuts at:   1020    1183    1683    3286    1020 
   Size:        163     500    1603    1864

  Fragments arranged by size: 

                1864    1603     500     163
 
PasI CC'CwG_GG

Cuts at:    589     589 
   Size:       4130
 
PciI A'CATG_T

Cuts at:   1016    3282    1016 
   Size:       2266    1864
 
PmeI GTTT'AAAC

Cuts at:   1129    1129 
   Size:       4130
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2576    2608    2576 
   Size:         32    4098
 
PpuMI rG'GwC_Cy

Cuts at:    570     570 
   Size:       4130
 
PstI C_TGCA'G

Cuts at:    493     953    1177    1862     493 
   Size:        460     224     685    2761

  Fragments arranged by size: 

                2761     685     460     224
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4130
 
PvuII CAG'CTG

Cuts at:    172     954    1555    1915     172 
   Size:        782     601     360    2387

  Fragments arranged by size: 

                2387     782     601     360
 
RsrII CG'GwC_CG

Cuts at:   2325    2325 
   Size:       4130
 
SacI G_AGCT'C

Cuts at:   1144    1144 
   Size:       4130
 
SalI G'TCGA_C

Cuts at:   1167    1167 
   Size:       4130
 
SapI GCTCTTCn'nnn_

Cuts at:   2153    2363    2153 
   Size:        210    3920
 
SbfI CC_TGCA'GG

Cuts at:   1177    1177 
   Size:       4130
 
ScaI AGT'ACT

Cuts at:   3647    3647 
   Size:       4130
 
SfcI C'TryA_G

Cuts at:     33     489     949    1173    1858    2826    3017      33 
   Size:        456     460     224     685     968     191    1146

  Fragments arranged by size: 

                1146     968     685     460     456     224     191
 
SfoI GGC'GCC

Cuts at:   1562    1810    1562 
   Size:        248    3882
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4130
 
SmaI CCC'GGG

Cuts at:    865    1152     865 
   Size:        287    3843
 
SmlI C'TyrA_G

Cuts at:   1285    2200    2914    3176    1285 
   Size:        915     714     262    2239

  Fragments arranged by size: 

                2239     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67     340      67 
   Size:        273    3857
 
SphI G_CATG'C

Cuts at:   1183    1683    1183 
   Size:        500    3630
 
StyI C'CwwG_G

Cuts at:    107     581    1100    1264    1618     107 
   Size:        474     519     164     354    2619

  Fragments arranged by size: 

                2619     519     474     354     164
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1713    1846    2305    2514    1713 
   Size:        133     459     209    3329

  Fragments arranged by size: 

                3329     459     209     133
 
TatI w'GTAC_w

Cuts at:    962    3645     962 
   Size:       2683    1447
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    113     145     330     362    3647    3679     113 
   Size:         32     185      32    3285      32     564

  Fragments arranged by size: 

                3285     564     185      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1927    1927 
   Size:       4130
 
XbaI T'CTAG_A

Cuts at:   1161    1161 
   Size:       4130
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    114    1625     114 
   Size:       1511    2619
 
XmaI C'CCGG_G

Cuts at:    863    1150     863 
   Size:        287    3843
 
XmnI GAAnn'nnTTC

Cuts at:    484     484 
   Size:       4130
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NspI
     PasI     PciI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI
    BmgBI   Bpu10I    BseRI    BsiWI     BsmI    BspEI    BsrGI   BssHII
   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI
    EcoRV     FseI    FspAI     HpaI     MfeI     NdeI     NotI     NruI
     NsiI     PacI    PflMI     PfoI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     ZraI
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