Restriction map for pF1KB8575
 (Linear) (Six-base) MAPSORT of: KIBB8575.seq  Check: 3255  from: 1  to: 1017



KIBB8575.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AccI GT'mk_AC

Cuts at:      0     407    1017 
   Size:        407     610
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     841    1017 
   Size:        841     176
 
AflIII A'CryG_T

Cuts at:      0     908    1017 
   Size:        908     109
 
AlwNI CAG_nnn'CTG

Cuts at:      0     465     778    1017 
   Size:        465     313     239

  Fragments arranged by size: 

                 465     313     239
 
ApaLI G'TGCA_C

Cuts at:      0      67     388    1017 
   Size:         67     321     629

  Fragments arranged by size: 

                 629     321      67
 
AvaI C'yCGr_G

Cuts at:      0     755    1017 
   Size:        755     262
 
BanI G'GyrC_C

Cuts at:      0     112    1017 
   Size:        112     905
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     574    1017 
   Size:        574     443
 
BclI T'GATC_A

Cuts at:      0     423    1017 
   Size:        423     594
 
BglI GCCn_nnn'nGGC

Cuts at:      0     670    1017 
   Size:        670     347
 
BlpI GC'TnA_GC

Cuts at:      0     967    1017 
   Size:        967      50
 
Bme1580I G_kGCm'C

Cuts at:      0      71     117     392    1017 
   Size:         71      46     275     625

  Fragments arranged by size: 

                 625     275      71      46
 
BmtI G_CTAG'C

Cuts at:      0     958    1017 
   Size:        958      59
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181    1017 
   Size:        181     836
 
BsaBI GATnn'nnATC

Cuts at:      0     437    1017 
   Size:        437     580
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       5      35     454     484    1017 
   Size:          5      30     419      30     533

  Fragments arranged by size: 

                 533     419      30      30       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205    1017 
   Size:        205     812
 
BsiHKAI G_wGCw'C

Cuts at:      0      71     392    1017 
   Size:         71     321     625

  Fragments arranged by size: 

                 625     321      71
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     991    1017 
   Size:        991      26
 
Bsp1286I G_dGCh'C

Cuts at:      0      71     117     392    1017 
   Size:         71      46     275     625

  Fragments arranged by size: 

                 625     275      71      46
 
BsrBI CCG'CTC

Cuts at:      0     630    1017 
   Size:        630     387
 
BsrDI GCAATG_nn'

Cuts at:      0     581     863    1017 
   Size:        581     282     154

  Fragments arranged by size: 

                 581     282     154
 
BstYI r'GATC_y

Cuts at:      0     356    1017 
   Size:        356     661
 
BtgI C'CryG_G

Cuts at:      0     473    1017 
   Size:        473     544
 
BtsI GCAGTG_nn'

Cuts at:      0     302     390     788     936    1017 
   Size:        302      88     398     148      81

  Fragments arranged by size: 

                 398     302     148      88      81
 
EaeI y'GGCC_r

Cuts at:      0    1001    1017 
   Size:       1001      16
 
EarI CTCTTCn'nnn_

Cuts at:      0      61      74    1017 
   Size:         61      13     943

  Fragments arranged by size: 

                 943      61      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     841    1017 
   Size:        181     660     176

  Fragments arranged by size: 

                 660     181     176
 
EcoO109I rG'GnC_Cy

Cuts at:      0     339     462    1017 
   Size:        339     123     555

  Fragments arranged by size: 

                 555     339     123
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     903     987    1017 
   Size:        903      84      30

  Fragments arranged by size: 

                 903      84      30
 
HaeII r_GCGC'y

Cuts at:      0     643    1017 
   Size:        643     374
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     454     486    1017 
   Size:        454      32     531

  Fragments arranged by size: 

                 531     454      32
 
HindIII A'AGCT_T

Cuts at:      0     574     969    1017 
   Size:        574     395      48

  Fragments arranged by size: 

                 574     395      48
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     896    1017 
   Size:        896     121
 
MslI CAynn'nnrTG

Cuts at:      0     793     913    1017 
   Size:        793     120     104

  Fragments arranged by size: 

                 793     120     104
 
MspA1I CmG'CkG

Cuts at:      0      64     134     846    1017 
   Size:         64      70     712     171

  Fragments arranged by size: 

                 712     171      70      64
 
NcoI C'CATG_G

Cuts at:      0     473    1017 
   Size:        473     544
 
NheI G'CTAG_C

Cuts at:      0     954    1017 
   Size:        954      63
 
NspI r_CATG'y

Cuts at:      0     912    1017 
   Size:        912     105
 
PasI CC'CwG_GG

Cuts at:      0     481    1017 
   Size:        481     536
 
PciI A'CATG_T

Cuts at:      0     908    1017 
   Size:        908     109
 
PpuMI rG'GwC_Cy

Cuts at:      0     462    1017 
   Size:        462     555
 
PstI C_TGCA'G

Cuts at:      0     385     845    1017 
   Size:        385     460     172

  Fragments arranged by size: 

                 460     385     172
 
PvuII CAG'CTG

Cuts at:      0      64     846    1017 
   Size:         64     782     171

  Fragments arranged by size: 

                 782     171      64
 
SfcI C'TryA_G

Cuts at:      0     381     841    1017 
   Size:        381     460     176

  Fragments arranged by size: 

                 460     381     176
 
SmaI CCC'GGG

Cuts at:      0     757    1017 
   Size:        757     260
 
SpeI A'CTAG_T

Cuts at:      0     232    1017 
   Size:        232     785
 
StyI C'CwwG_G

Cuts at:      0     473     992    1017 
   Size:        473     519      25

  Fragments arranged by size: 

                 519     473      25
 
TatI w'GTAC_w

Cuts at:      0     854    1017 
   Size:        854     163
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       5      37     222     254    1017 
   Size:          5      32     185      32     763

  Fragments arranged by size: 

                 763     185      32      32       5
 
XmaI C'CCGG_G

Cuts at:      0     755    1017 
   Size:        755     262
 
XmnI GAAnn'nnTTC

Cuts at:      0     376    1017 
   Size:        376     641
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI    ApaLI     AvaI     BanI     BbsI
     BclI     BglI     BlpI Bme1580I     BmtI     BpmI    BsaBI    BsaXI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BsrBI    BsrDI    BstYI     BtgI
     BtsI     EaeI     EarI  Eco57MI EcoO109I     FalI    HaeII    Hin4I
  HindIII     MmeI     MslI   MspA1I     NcoI     NheI     NspI     PasI
     PciI    PpuMI     PstI    PvuII     SfcI     SmaI     SpeI     StyI
     TatI     TstI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI
    BfrBI    BglII    BmgBI     BmrI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BseRI    BseYI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BspMI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NotI
     NruI     NsiI     PacI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SphI     SrfI     SspI
     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com