Restriction map for pF1KB8569
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflIII      
AhdI        Alw44I      ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BbeI        BbrPI       BcgI        BciVI       
BclI        BglII       BlnI        BsaAI       BsaBI       
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspLU11I    BsrGI       BssHII      Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco52I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PflFI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AcsI         1621  AAATTC               [GA]^AATT[CT]
AflII        1783  CTTAAG               C^TTAAG
AgeI         2081  ACCGGT               A^CCGGT
Aor51HI       983  AGCGCT               AGC^GCT
ApoI         1621  AAATTC               [GA]^AATT[CT]
BfrI         1783  CTTAAG               C^TTAAG
BsmBI         958  CGTCTC               CGTCTC(1/5)
BspHI        1986  TCATGA               T^CATGA
BsrDI        1302  CATTGC               GCAATG(2/0)
BssSI         142  CACGAG               CACGAG(-5/-1)
BstAPI        122  GCAGCCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1699  GGTGACC              G^GT[ACGT]ACC
BstPI        1699  GGTGACC              G^GT[ACGT]ACC
DrdI          511  GACAGCTGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      983  AGCGCT               AGC^GCT
EcoO65I      1699  GGTGACC              G^GT[ACGT]ACC
HindIII      1780  AAGCTT               A^AGCTT
MseI         1784  TTAA                 T^TAA
NcoI          204  CCATGG               C^CATGG
PaeR7I        970  CTCGAG               C^TCGAG
PinAI        2081  ACCGGT               A^CCGGT
PshAI          55  GACCCCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1986  TCATGA               T^CATGA
SacI          862  GAGCTC               GAGCT^C
SexAI        1862  ACCTGGT              A^CC[AT]GGT
SmaI          382  CCCGGG               CCC^GGG
Tru9I        1784  TTAA                 T^TAA
XcmI         1317  CCAGCTGAATGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          970  CTCGAG               C^TCGAG
XmaCI         382  CCCGGG               C^CCGGG
XmaI          382  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccII        1478  CGCG                 CG^CG
             1720  CGCG                
AccIII         89  TCCGGA               T^CCGGA
             2228  TCCGGA              
AcyI         1549  GGCGTC               G[GA]^CG[CT]C
             1593  GACGCC              
ApaI          560  GGGCCC               GGGCC^C
              759  GGGCCC              
BamHI        1214  GGATCC               G^GATCC
             1847  GGATCC              
BfaI          241  CTAG                 C^TAG
              481  CTAG                
BlpI          489  GCTGAGC              GC^T[ACGT]AGC
              864  GCTCAGC             
Bpu1102I      489  GCTGAGC              GC^T[ACGT]AGC
              864  GCTCAGC             
BsaHI        1549  GGCGTC               G[GA]^CG[CT]C
             1593  GACGCC              
BsaI         2125  GAGACC               GGTCTC(1/5)
             1982  GGTCTC              
BseAI          89  TCCGGA               T^CCGGA
             2228  TCCGGA              
BspEI          89  TCCGGA               T^CCGGA
             2228  TCCGGA              
BsrFI        1169  GCCGGT               [GA]^CCGG[CT]
             2081  ACCGGT              
BstUI        1478  CGCG                 CG^CG
             1720  CGCG                
BstYI        1214  GGATCC               [GA]^GATC[CT]
             1847  GGATCC              
CelII         489  GCTGAGC              GC^T[ACGT]AGC
              864  GCTCAGC             
Cfr10I       1169  GCCGGT               [GA]^CCGG[CT]
             2081  ACCGGT              
DsaI          204  CCATGG               C^C[GA][CT]GG
             1599  CCGTGG              
EarI         1810  CTCTTC               CTCTTC(1/4)
               73  GAAGAG              
Hin1I        1549  GGCGTC               G[GA]^CG[CT]C
             1593  GACGCC              
Ksp632I      1810  CTCTTC               CTCTTC(1/4)
               73  GAAGAG              
MaeI          241  CTAG                 C^TAG
              481  CTAG                
MaeII         418  ACGT                 A^CGT
             1772  ACGT                
MflI         1214  GGATCC               [GA]^GATC[CT]
             1847  GGATCC              
MroI           89  TCCGGA               T^CCGGA
             2228  TCCGGA              
MvnI         1478  CGCG                 CG^CG
             1720  CGCG                
SmlI          970  CTCGAG               C^T[CT][GA]AG
             1783  CTTAAG              
StuI         1109  AGGCCT               AGG^CCT
             1675  AGGCCT              
Tsp509I      1298  AATT                 ^AATT
             1622  AATT                
XhoII        1214  GGATCC               [GA]^GATC[CT]
             1847  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AocI        Asp700I     AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvCI       
BbvI        BcnI        BglI        BmyI        BpmI        
BpuAI       BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrI        
BssKI       BstF5I      BstNI       BstXI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfiI        StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XmnI        
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