Restriction map for pF1KB8462
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       AccI        AccII       AclI        AcyI        
AflII       AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AvaI        AviII       
BalI        BamHI       BbeI        BbsI        BbvCI       
BciVI       BclI        BfrI        BglII       BlpI        
BpmI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaI        BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       BspDI       BspHI       BspLU11I    BspMI       
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoRV       EcoT22I     FbaI        FseI        
FspI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KspI        MamI        MfeI        
MluI        MluNI       MscI        MslI        MunI        
MvnI        NarI        NcoI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PleI        PmeI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
StuI        SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   68 enzymes) #
###################################

Acc65I        852  GGTACC               G^GTACC
AccIII        642  TCCGGA               T^CCGGA
AflIII        561  ACGTGT               A^C[GA][CT]GT
AgeI          160  ACCGGT               A^CCGGT
AlwI          241  GGATC                GGATC(4/5)
AlwNI         986  CAGCCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I       852  GGTACC               G^GTACC
AspHI         263  GAGCAC               G[AT]GC[AT]^C
AvrII         684  CCTAGG               C^CTAGG
BanII         803  GAGCCC               G[GA]GC[CT]^C
BbrPI         560  CACGTG               CAC^GTG
BcnI          366  CCGGG                CC^[GC]GG
BglI          778  GCCACCACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          684  CCTAGG               C^CTAGG
BsaAI         560  CACGTG               [CT]AC^GT[GA]
BseAI         642  TCCGGA               T^CCGGA
BseRI         800  GAGGAG               GAGGAG(10/8)
BsgI          839  GTGCAG               GTGCAG(16/14)
BsiHKAI       263  GAGCAC               G[AT]GC[AT]^C
BsmAI         343  GTCTC                GTCTC(1/5)
Bsp1407I     1025  TGTACA               T^GTACA
BspEI         642  TCCGGA               T^CCGGA
BsrBI         848  GAGCGG               CCGCTC(-3/-3)
BsrGI        1025  TGTACA               T^GTACA
BsrI          619  CCAGT                ACTGG(1/-1)
BstEII         28  GGTAACC              G^GT[ACGT]ACC
BstPI          28  GGTAACC              G^GT[ACGT]ACC
BstYI         241  GGATCT               [GA]^GATC[CT]
CfoI         1079  GCGC                 GCG^C
DpnII         242  GATC                 ^GATC
DraII         368  GGGACCC              [GA]G^G[ACGT]CC[CT]
EarI          716  GAAGAG               CTCTTC(1/4)
EcoO109I      368  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I        28  GGTAACC              G^GT[ACGT]ACC
EcoRI         110  GAATTC               G^AATTC
EcoT14I       684  CCTAGG               C^C[AT][AT]GG
FauI          871  CCCGC                CCCGC(4/6)
HaeII        1078  GGCGCT               [GA]GCGC^[CT]
HgaI           55  GACGC                GACGC(5/10)
HhaI         1079  GCGC                 GCG^C
HinP1I       1079  GCGC                 G^CGC
HinfI         452  GATTC                G^A[ACGT]TC
KpnI          852  GGTACC               GGTAC^C
Ksp632I       716  GAAGAG               CTCTTC(1/4)
MboI          242  GATC                 ^GATC
MflI          241  GGATCT               [GA]^GATC[CT]
MroI          642  TCCGGA               T^CCGGA
NaeI          151  GCCGGC               GCC^GGC
NciI          366  CCGGG                CC^[GC]GG
NdeI          593  CATATG               CA^TATG
NdeII         242  GATC                 ^GATC
NgoMIV        151  GCCGGC               G^CCGGC
NheI         1063  GCTAGC               G^CTAGC
NspI          339  GCATGT               [GA]CATG^[CT]
PflMI         619  CCAGTAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         160  ACCGGT               A^CCGGT
PmaCI         560  CACGTG               CAC^GTG
PmlI          560  CACGTG               CAC^GTG
PpuMI         368  GGGACCC              [GA]G^G[AT]CC[CT]
Sau3AI        242  GATC                 ^GATC
SmlI          140  CTCAAG               C^T[CT][GA]AG
SspBI        1025  TGTACA               T^GTACA
SspI          349  AATATT               AAT^ATT
StyI          684  CCTAGG               C^C[AT][AT]GG
TfiI          452  GATTC                G^A[AT]TC
Van91I        619  CCAGTAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          572  CCAATAAGAAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         241  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcsI          110  GAATTC               [GA]^AATT[CT]
              306  AAATTC              
ApoI          110  GAATTC               [GA]^AATT[CT]
              306  AAATTC              
AvaII         369  GGACC                G^G[AT]CC
              640  GGTCC               
BcgI          329  CGAAACGGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              471  CGAGAATAGTGC        
BsaWI         160  ACCGGT               [AT]^CCGG[AT]
              642  TCCGGA              
BsmFI         368  GGGAC                GGGAC(10/14)
              681  GTCCC               
BsrDI          58  GCAATG               GCAATG(2/0)
              689  GCAATG              
BsrFI         151  GCCGGC               [GA]^CCGG[CT]
              160  ACCGGT              
BstAPI        949  GCACAGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              985  GCAGCCGCTGC         
Cfr10I        151  GCCGGC               [GA]^CCGG[CT]
              160  ACCGGT              
DdeI           23  CTCAG                C^T[ACGT]AG
              446  CTAAG               
EaeI          153  CGGCCA               [CT]^GGCC[GA]
              276  CGGCCA              
MaeII         438  ACGT                 A^CGT
              561  ACGT                
MboII         579  GAAGA                GAAGA(8/7)
              716  GAAGA               
MspA1I        977  CCGCTG               C[AC]G^C[GT]G
              989  CCGCTG              
NlaIII        340  CATG                 CATG^
             1045  CATG                
TaqI          470  TCGA                 T^CGA
              539  TCGA                
TspRI         701  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1005  CAGTG               
TthHB8I       470  TCGA                 T^CGA
              539  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AciI        AfaI        AluI        BanI        BbvI        
BfaI        BmyI        BsaJI       BsiYI       BslI        
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DsaI        EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HpaII       HphI        ItaI        
MaeI        MaeIII      MnlI        MseI        MspI        
MvaI        MwoI        NlaIV       PstI        RsaI        
Sau96I      ScrFI       SfaNI       SfcI        Tru9I       
TseI        Tsp45I      Tsp509I     
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