Restriction map for pF1KB8460
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BclI        BfaI        BfrI        BglI        
BlnI        BsaBI       BsaHI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Cfr10I      ClaI        CpoI        DraI        DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        Hin1I       HincII      
HindII      HindIII     HpaI        HphI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeIII      
MamI        MfeI        MluI        MroI        MseI        
MslI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TfiI        
Tru9I       Tsp45I      Tsp509I     Tth111I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccII         106  CGCG                 CG^CG
AviII         695  TGCGCA               TGC^GCA
BalI          117  TGGCCA               TGG^CCA
BbsI          622  GTCTTC               GAAGAC(2/6)
BciVI         182  GTATCC               GTATCC(6/5)
BglII         138  AGATCT               A^GATCT
BlpI          487  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      487  GCTGAGC              GC^T[ACGT]AGC
BpuAI         622  GTCTTC               GAAGAC(2/6)
BsaAI         179  TACGTA               [CT]AC^GT[GA]
BsaI          253  GAGACC               GGTCTC(1/5)
BsaWI         650  ACCGGA               [AT]^CCGG[AT]
BseRI         250  GAGGAG               GAGGAG(10/8)
BsgI          319  CTGCAC               GTGCAG(16/14)
BsmFI         741  GGGAC                GGGAC(10/14)
BspHI         731  TCATGA               T^CATGA
BspMI         575  GCAGGT               ACCTGC(4/8)
BstUI         106  CGCG                 CG^CG
BstYI         138  AGATCT               [GA]^GATC[CT]
CelII         487  GCTGAGC              GC^T[ACGT]AGC
DpnII         139  GATC                 ^GATC
DraIII        607  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
FspI          695  TGCGCA               TGC^GCA
MaeII         180  ACGT                 A^CGT
MboI          139  GATC                 ^GATC
MboII         623  TCTTC                GAAGA(8/7)
MflI          138  AGATCT               [GA]^GATC[CT]
MluNI         117  TGGCCA               TGG^CCA
MscI          117  TGGCCA               TGG^CCA
MvnI          106  CGCG                 CG^CG
NdeII         139  GATC                 ^GATC
RcaI          731  TCATGA               T^CATGA
Sau3AI        139  GATC                 ^GATC
SfaNI         547  GATGC                GCATC(5/9)
SmaI          232  CCCGGG               CCC^GGG
SnaBI         179  TACGTA               TAC^GTA
StuI          189  AGGCCT               AGG^CCT
XcmI          155  CCAGCAGACTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         138  AGATCT               [GA]^GATC[CT]
XmaCI         232  CCCGGG               C^CCGGG
XmaI          232  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI            7  GTAC                 GT^AC
              102  GTAC                
AlwNI         463  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              676  CAGGCCCTG           
AocI          258  CCTGAGG              CC^T[ACGT]AGG
              681  CCTGAGG             
AspHI         109  GTGCTC               G[AT]GC[AT]^C
              214  GAGCAC              
AvaI          232  CCCGGG               C^[CT]CG[GA]G
              430  CTCGGG              
BsiHKAI       109  GTGCTC               G[AT]GC[AT]^C
              214  GAGCAC              
BsoBI         232  CCCGGG               C^[CT]CG[GA]G
              430  CTCGGG              
Bsu36I        258  CCTGAGG              CC^T[ACGT]AGG
              681  CCTGAGG             
Eco81I        258  CCTGAGG              CC^T[ACGT]AGG
              681  CCTGAGG             
EcoNI         348  CCTCCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              681  CCTGAGGAAGG         
FauI          276  GCGGG                CCCGC(4/6)
              618  GCGGG               
HaeII         227  AGCGCC               [GA]GCGC^[CT]
              344  AGCGCC              
HgaI           24  GACGC                GACGC(5/10)
              314  GCGTC               
HinfI         161  GACTC                G^A[ACGT]TC
              728  GACTC               
PleI          161  GACTC                GAGTC(4/5)
              728  GACTC               
RsaI            7  GTAC                 GT^AC
              102  GTAC                
TaqI           94  TCGA                 T^CGA
              389  TCGA                
TthHB8I        94  TCGA                 T^CGA
              389  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AluI        AvaII       BanII       BbvI        
BcnI        BmyI        BpmI        BsaJI       BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DraII       EaeI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HpaII       ItaI        MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NlaIII      NlaIV       PflMI       PstI        PvuII       
Sau96I      ScrFI       SfcI        StyI        TseI        
TspRI       Van91I      
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