Restriction map for pF1KB8436
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbsI        BbvCI       BcgI        BfrI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmAI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
EagI        Eam1105I    EarI        EclXI       Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRV       EcoT22I     
FseI        FspI        HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacII       
SapI        SbfI        ScaI        SexAI       SfcI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI          865  GTCGAC               GT^[AC][GT]AC
AccII         496  CGCG                 CG^CG
AcsI          919  GAATTC               [GA]^AATT[CT]
AcyI          103  GACGCC               G[GA]^CG[CT]C
Aor51HI       690  AGCGCT               AGC^GCT
ApoI          919  GAATTC               [GA]^AATT[CT]
BanII         115  GAGCTC               G[GA]GC[CT]^C
BbrPI         984  CACGTG               CAC^GTG
BglI          256  GCCATGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         828  AGATCT               A^GATCT
BsaAI         984  CACGTG               [CT]AC^GT[GA]
BsaBI         174  GATGAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         103  GACGCC               G[GA]^CG[CT]C
BsmI          427  GAATGC               GAATGC(1/-1)
BspMI         515  ACCTGC               ACCTGC(4/8)
BsrBI          76  GAGCGG               CCGCTC(-3/-3)
BsrI         1047  CCAGT                ACTGG(1/-1)
BssSI         715  CTCGTG               CACGAG(-5/-1)
BstUI         496  CGCG                 CG^CG
DrdI            3  GACTCTGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          254  CGGCCA               [CT]^GGCC[GA]
Eco47III      690  AGCGCT               AGC^GCT
EcoNI          30  CCTGAGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         919  GAATTC               G^AATTC
HaeII         690  AGCGCT               [GA]GCGC^[CT]
HgaI          103  GACGC                GACGC(5/10)
Hin1I         103  GACGCC               G[GA]^CG[CT]C
HincII        865  GTCGAC               GT[CT]^[GA]AC
HindII        865  GTCGAC               GT[CT]^[GA]AC
MamI          174  GATGAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          252  CACGGCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          496  CGCG                 CG^CG
NdeI          380  CATATG               CA^TATG
PmaCI         984  CACGTG               CAC^GTG
PmlI          984  CACGTG               CAC^GTG
SacI          115  GAGCTC               GAGCT^C
SalI          865  GTCGAC               G^TCGAC
SfiI          255  GGCCATGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
XbaI          834  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AfaI          158  GTAC                 GT^AC
             1065  GTAC                
AspHI         115  GAGCTC               G[AT]GC[AT]^C
              310  GAGCAC              
AvaI          133  CTCGGG               C^[CT]CG[GA]G
              914  CCCGAG              
BciVI         196  GGATAC               GTATCC(6/5)
              647  GGATAC              
BclI          269  TGATCA               T^GATCA
              705  TGATCA              
BcnI           53  CCCGG                CC^[GC]GG
              111  CCGGG               
BmyI          115  GAGCTC               G[AGT]GC[ACT]^C
              310  GAGCAC              
BpmI         1045  CTCCAG               CTGGAG(16/14)
                7  CTGGAG              
BsiHKAI       115  GAGCTC               G[AT]GC[AT]^C
              310  GAGCAC              
BsoBI         133  CTCGGG               C^[CT]CG[GA]G
              914  CCCGAG              
Bsp1286I      115  GAGCTC               G[AGT]GC[ACT]^C
              310  GAGCAC              
BstF5I        183  CATCC                GGATG(2/0)
             1041  CATCC               
BstYI         293  AGATCC               [GA]^GATC[CT]
              828  AGATCT              
CfoI          691  GCGC                 GCG^C
              989  GCGC                
DsaI          186  CCATGG               C^C[GA][CT]GG
              278  CCATGG              
FauI          107  CCCGC                CCCGC(4/6)
               99  GCGGG               
FbaI          269  TGATCA               T^GATCA
              705  TGATCA              
FokI          183  CATCC                GGATG(9/13)
             1041  CATCC               
HapII          54  CCGG                 C^CGG
              111  CCGG                
HhaI          691  GCGC                 GCG^C
              989  GCGC                
HinP1I        691  GCGC                 G^CGC
              989  GCGC                
HpaII          54  CCGG                 C^CGG
              111  CCGG                
HphI          224  TCACC                GGTGA(8/7)
              668  TCACC               
MaeII         160  ACGT                 A^CGT
              985  ACGT                
MaeIII        667  GTCAC                ^GT[ACGT]AC
              959  GTGAC               
MflI          293  AGATCC               [GA]^GATC[CT]
              828  AGATCT              
MseI          482  TTAA                 T^TAA
              817  TTAA                
MspI           54  CCGG                 C^CGG
              111  CCGG                
NciI           53  CCCGG                CC^[GC]GG
              111  CCGGG               
NcoI          186  CCATGG               C^CATGG
              278  CCATGG              
RsaI          158  GTAC                 GT^AC
             1065  GTAC                
SfaNI         388  GATGC                GCATC(5/9)
              901  GATGC               
SmlI          118  CTCAAG               C^T[CT][GA]AG
              289  CTCAAG              
Tru9I         482  TTAA                 T^TAA
              817  TTAA                
Tsp45I        667  GTCAC                ^GT[GC]AC
              959  GTGAC               
TspRI         652  CACTG                CA[GC]TG[ACGT][ACGT]^
              957  CAGTG               
XhoII         293  AGATCC               [GA]^GATC[CT]
              828  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AluI        AlwI        Asp700I     AvaII       
BbvI        BfaI        BsaJI       BseRI       BsiYI       
BslI        BsmFI       BssKI       BstNI       Cac8I       
Cfr13I      DdeI        DpnII       EcoRII      EcoT14I     
Fnu4HI      HaeIII      HinfI       ItaI        MaeI        
MboI        MboII       MnlI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
Sau96I      ScrFI       StyI        TaqI        TfiI        
TseI        Tsp509I     TthHB8I     XmnI        
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