Restriction map for pF1KB8430
##################################
# NO CUT ENZYMES (  185 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HaeIII      HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccII           9  CGCG                 CG^CG
AcsI          297  GAATTT               [GA]^AATT[CT]
AfaI          468  GTAC                 GT^AC
ApoI          297  GAATTT               [GA]^AATT[CT]
BanII         421  GAGCCC               G[GA]GC[CT]^C
BbsI           73  GAAGAC               GAAGAC(2/6)
BmyI          421  GAGCCC               G[AGT]GC[ACT]^C
BpuAI          73  GAAGAC               GAAGAC(2/6)
BsiEI          14  CGACCG               CG[GA][CT]^CG
BsiYI         425  CCCATGCACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          425  CCCATGCACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         370  GTCCC                GGGAC(10/14)
Bsp1286I      421  GAGCCC               G[AGT]GC[ACT]^C
BsrI          318  ACTGG                ACTGG(1/-1)
BstUI           9  CGCG                 CG^CG
Cac8I         700  GCTGGC               GC[ACGT]^[ACGT]GC
EarI          167  GAAGAG               CTCTTC(1/4)
EcoT14I       147  CCTTGG               C^C[AT][AT]GG
HapII         116  CCGG                 C^CGG
HpaII         116  CCGG                 C^CGG
HphI          379  GGTGA                GGTGA(8/7)
Ksp632I       167  GAAGAG               CTCTTC(1/4)
MaeIII        547  GTCAC                ^GT[ACGT]AC
MslI          354  CATGGTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        698  CAGCTG               C[AC]G^C[GT]G
MspI          116  CCGG                 C^CGG
MvnI            9  CGCG                 CG^CG
NlaIV         618  GGCTCC               GG[ACGT]^[ACGT]CC
PleI          170  GAGTC                GAGTC(4/5)
PvuII         698  CAGCTG               CAG^CTG
RsaI          468  GTAC                 GT^AC
SfcI          492  CTACAG               C^T[GA][CT]AG
SspI          282  AATATT               AAT^ATT
StyI          147  CCTTGG               C^C[AT][AT]GG
TfiI           96  GAATC                G^A[AT]TC
Tsp45I        547  GTCAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AciI           17  CCGC                 CCGC(-3/-1)
               10  GCGG                
AluI          444  AGCT                 AG^CT
              699  AGCT                
Asp700I       152  GAACAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              625  GAAATAATTC          
BfaI           58  CTAG                 C^TAG
              481  CTAG                
BglII         218  AGATCT               A^GATCT
              498  AGATCT              
BssKI         373  CCTGG                ^CC[ACGT]GG
              543  CCAGG               
BstNI         373  CCTGG                CC^[AT]GG
              543  CCAGG               
BstYI         218  AGATCT               [GA]^GATC[CT]
              498  AGATCT              
DpnII         219  GATC                 ^GATC
              499  GATC                
EcoRII        373  CCTGG                ^CC[AT]GG
              543  CCAGG               
HinfI          96  GAATC                G^A[ACGT]TC
              170  GAGTC               
MaeI           58  CTAG                 C^TAG
              481  CTAG                
MboI          219  GATC                 ^GATC
              499  GATC                
MflI          218  AGATCT               [GA]^GATC[CT]
              498  AGATCT              
MvaI          373  CCTGG                CC^[AT]GG
              543  CCAGG               
MwoI           10  GCGGCGACCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              252  GCAAATAGAGC         
NdeII         219  GATC                 ^GATC
              499  GATC                
Sau3AI        219  GATC                 ^GATC
              499  GATC                
ScrFI         373  CCTGG                CC^[ACGT]GG
              543  CCAGG               
TaqI          315  TCGA                 T^CGA
              464  TCGA                
TthHB8I       315  TCGA                 T^CGA
              464  TCGA                
XhoII         218  AGATCT               [GA]^GATC[CT]
              498  AGATCT              
XmnI          152  GAACAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              625  GAAATAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

BbvI        BpmI        BsaJI       DdeI        Fnu4HI      
ItaI        MboII       MnlI        MseI        NlaIII      
Tru9I       TseI        Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com