Restriction map for pF1KB8422
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaII       AviII       AvrII       BamHI       BbeI        
BbvCI       BciVI       BfrI        BglI        BglII       
BlnI        BsaBI       BsaHI       BseAI       BsiWI       
BsmBI       BsmFI       BsmI        Bsp1407I    BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       BssHII      
Bst1107I    BstXI       BstZ17I     CpoI        DraI        
DraIII      DrdI        Eam1105I    EcoRV       FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        MamI        MfeI        
MroI        MunI        NarI        NcoI        NdeI        
NheI        NruI        PacI        PaeR7I      PflFI       
PinAI       PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SbfI        ScaI        SfiI        SfoI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Alw44I        846  GTGCAC               G^TGCAC
AlwNI        1839  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1743  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       752  AGCGCT               AGC^GCT
ApaLI         846  GTGCAC               G^TGCAC
BalI         1382  TGGCCA               TGG^CCA
BanI         1800  GGTGCC               G^G[CT][GA]CC
BbrPI        1399  CACGTG               CAC^GTG
BcgI         1214  CGAGCCAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI           20  TGATCA               T^GATCA
BlpI          876  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      876  GCTCAGC              GC^T[ACGT]AGC
BsaWI        1544  ACCGGA               [AT]^CCGG[AT]
BsiEI        1026  CGGCCG               CG[GA][CT]^CG
BspDI         712  ATCGAT               AT^CGAT
BsrBI        1678  GAGCGG               CCGCTC(-3/-3)
BssSI        1849  CACGAG               CACGAG(-5/-1)
BstBI         211  TTCGAA               TT^CGAA
BstYI        1938  GGATCT               [GA]^GATC[CT]
Bsu36I       1743  CCTGAGG              CC^T[ACGT]AGG
CelII         876  GCTCAGC              GC^T[ACGT]AGC
ClaI          712  ATCGAT               AT^CGAT
EagI         1026  CGGCCG               C^GGCCG
EclXI        1026  CGGCCG               C^GGCCG
Eco47III      752  AGCGCT               AGC^GCT
Eco52I       1026  CGGCCG               C^GGCCG
Eco81I       1743  CCTGAGG              CC^T[ACGT]AGG
EcoNI         175  CCTCCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1537  GAATTC               G^AATTC
EcoT22I      2006  ATGCAT               ATGCA^T
FbaI           20  TGATCA               T^GATCA
HindIII      1522  AAGCTT               A^AGCTT
KspI         1029  CCGCGG               CCGC^GG
MflI         1938  GGATCT               [GA]^GATC[CT]
MluI          842  ACGCGT               A^CGCGT
MluNI        1382  TGGCCA               TGG^CCA
MscI         1382  TGGCCA               TGG^CCA
MseI          464  TTAA                 T^TAA
NotI         1025  GCGGCCGC             GC^GGCCGC
NsiI         2006  ATGCAT               ATGCA^T
NspV          211  TTCGAA               TT^CGAA
PmaCI        1399  CACGTG               CAC^GTG
PmlI         1399  CACGTG               CAC^GTG
SacI         1051  GAGCTC               GAGCT^C
SacII        1029  CCGCGG               CCGC^GG
SexAI         800  ACCTGGT              A^CC[AT]GGT
SfuI          211  TTCGAA               TT^CGAA
SphI          242  GCATGC               GCATG^C
StuI          947  AGGCCT               AGG^CCT
TfiI         1715  GAATC                G^A[AT]TC
Tru9I         464  TTAA                 T^TAA
XhoII        1938  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcsI         1537  GAATTC               [GA]^AATT[CT]
             1621  AAATTC              
AflIII        842  ACGCGT               A^C[GA][CT]GT
             1398  ACACGT              
ApaI         1164  GGGCCC               GGGCC^C
             2130  GGGCCC              
ApoI         1537  GAATTC               [GA]^AATT[CT]
             1621  AAATTC              
BbsI           60  GAAGAC               GAAGAC(2/6)
              208  GTCTTC              
BfaI          190  CTAG                 C^TAG
              227  CTAG                
BpuAI          60  GAAGAC               GAAGAC(2/6)
              208  GTCTTC              
BsaAI         727  TACGTG               [CT]AC^GT[GA]
             1399  CACGTG              
BsaI          185  GAGACC               GGTCTC(1/5)
              670  GAGACC              
BstAPI        848  GCACGCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1838  GCAGCCACTGC         
BstEII        804  GGTCACC              G^GT[ACGT]ACC
             2041  GGTGACC             
BstPI         804  GGTCACC              G^GT[ACGT]ACC
             2041  GGTGACC             
DsaI          318  CCACGG               C^C[GA][CT]GG
             1029  CCGCGG              
EcoO65I       804  GGTCACC              G^GT[ACGT]ACC
             2041  GGTGACC             
MaeI          190  CTAG                 C^TAG
              227  CTAG                
NaeI          596  GCCGGC               GCC^GGC
             2080  GCCGGC              
NgoMIV        596  GCCGGC               G^CCGGC
             2080  GCCGGC              
PflMI         810  CCACATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1695  CCAAGGTCTGG         
PvuII        1135  CAGCTG               CAG^CTG
             1219  CAGCTG              
SapI          237  GAAGAGC              GCTCTTC(1/4)
              864  GCTCTTC             
SmaI          202  CCCGGG               CCC^GGG
              543  CCCGGG              
Van91I        810  CCACATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1695  CCAAGGTCTGG         
XcmI          674  CCAAGCAGAAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              791  CCAAGTACAACCTGG     
XmaCI         202  CCCGGG               C^CCGGG
              543  CCCGGG              
XmaI          202  CCCGGG               C^CCGGG
              543  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
Asp700I     AspHI       AvaI        BanII       BbvI        
BcnI        BmyI        BpmI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsoBI       Bsp1286I    BspMI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XmnI        
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