Restriction map for
pF1KB8422
(Circular) (Six-base) MAPSORT of: pF1KB8422.seq Check: 3537 from: 1 to: 5345
pF1KB8422 5345 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 886 1001 1691 886
Size: 115 690 4540
Fragments arranged by size:
4540 690 115
AccI GT'mk_AC
Cuts at: 2383 2383
Size: 5345
Acc65I G'GTAC_C
Cuts at: 2361 2361
Size: 5345
AclI AA'CG_TT
Cuts at: 5185 5185
Size: 5345
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 684 1099 1267 1759 3170 3602 3955 4617 684
Size: 415 168 492 1411 432 353 662 1412
Fragments arranged by size:
1412 1411 662 492 432 415 353 168
AfeI AGC'GCT
Cuts at: 862 862
Size: 5345
AflIII A'CryG_T
Cuts at: 950 1506 2697 4497 4778 950
Size: 556 1191 1800 281 1517
Fragments arranged by size:
1800 1517 1191 556 281
AleI CACnn'nnGTG
Cuts at: 1187 1439 1187
Size: 252 5093
AlwNI CAG_nnn'CTG
Cuts at: 1952 2650 4088 1952
Size: 698 1438 3209
Fragments arranged by size:
3209 1438 698
ApaI G_GGCC'C
Cuts at: 1276 2242 1276
Size: 966 4379
ApaLI G'TGCA_C
Cuts at: 954 4183 954
Size: 3229 2116
ApoI r'AATT_y
Cuts at: 1645 1729 2349 4698 4954 1645
Size: 84 620 2349 256 2036
Fragments arranged by size:
2349 2036 620 256 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 5345
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5345
AvaI C'yCGr_G
Cuts at: 209 310 651 1320 2041 2074 2365 209
Size: 101 341 669 721 33 291 3189
Fragments arranged by size:
3189 721 669 341 291 101 33
BamHI G'GATC_C
Cuts at: 2370 2370
Size: 5345
BanI G'GyrC_C
Cuts at: 1908 2361 2775 3023 3058 1908
Size: 453 414 248 35 4195
Fragments arranged by size:
4195 453 414 248 35
BanII G_rGCy'C
Cuts at: 764 1163 1276 1877 1997 2242 2359 3389 764
Size: 399 113 601 120 245 117 1030 2720
Fragments arranged by size:
2720 1030 601 399 245 120 117 113
BbeI G_GCGC'C
Cuts at: 2779 3027 2779
Size: 248 5097
BbsI GAAGACnn'nnnn_
Cuts at: 175 309 175
Size: 134 5211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1311 1345 90
Size: 34 1187 34 4090
Fragments arranged by size:
4090 1187 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3243 4299 5345 3243
Size: 1056 1046 3243
Fragments arranged by size:
3243 1056 1046
BclI T'GATC_A
Cuts at: 128 2865 128
Size: 2737 2608
BfrBI ATG'CAT
Cuts at: 2116 2116
Size: 5345
BglII A'GATC_T
Cuts at: 2860 4897 2860
Size: 2037 3308
BlpI GC'TnA_GC
Cuts at: 985 2457 985
Size: 1472 3873
Bme1580I G_kGCm'C
Cuts at: 680 874 958 1024 1276 1941 2242 2970 3063
Size: 194 84 66 252 665 301 728 93
Cuts at: 3063 4187 680
Size: 1124 1838
Fragments arranged by size:
1838 1124 728 665 301 252 194 93
84 66
BmgBI CAC'GTC
Cuts at: 807 843 1611 807
Size: 36 768 4541
Fragments arranged by size:
4541 768 36
BmrI ACTGGGnnnn_n'
Cuts at: 261 2557 2638 2973 5117 261
Size: 2296 81 335 2144 489
Fragments arranged by size:
2296 2144 489 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3712 3712
Size: 5345
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1882 2104 2320 1882
Size: 222 216 4907
Fragments arranged by size:
4907 222 216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1147 1399 1567 2521 3400 4114 4412 1147
Size: 252 168 954 879 714 298 2080
Fragments arranged by size:
2080 954 879 714 298 252 168
BsaI GGTCTCn'nnnn_
Cuts at: 287 772 5049 287
Size: 485 4277 583
Fragments arranged by size:
4277 583 485
BsaAI yAC'GTr
Cuts at: 837 1509 2698 837
Size: 672 1189 3484
Fragments arranged by size:
3484 1189 672
BsaBI GATnn'nnATC
Cuts at: 6 2859 4896 6
Size: 2853 2037 455
Fragments arranged by size:
2853 2037 455
BsaHI Gr'CG_yC
Cuts at: 2776 3024 3726 5222 2776
Size: 248 702 1496 2899
Fragments arranged by size:
2899 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1652 2534 3055 3733 4144 4291 4565 1652
Size: 882 521 678 411 147 274 2432
Fragments arranged by size:
2432 882 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 591 621 1276 1306 1441 1471 2133 2163 591
Size: 30 655 30 135 30 662 30 3773
Fragments arranged by size:
3773 662 655 135 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 427 790 1869 1885 1975 1984 2116 2154 427
Size: 363 1079 16 90 9 132 38 3618
Fragments arranged by size:
3618 1079 363 132 90 38 16 9
BseYI C'CCAG_C
Cuts at: 577 593 1153 1241 2770 3528 4193 4601 577
Size: 16 560 88 1529 758 665 408 1321
Fragments arranged by size:
1529 1321 758 665 560 408 88 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 1420 1588 1669 2019 835
Size: 585 168 81 350 4161
Fragments arranged by size:
4161 585 350 168 81
BsiEI CG_ry'CG
Cuts at: 104 1137 2887 2933 4163 104
Size: 1033 1750 46 1230 1286
Fragments arranged by size:
1750 1286 1230 1033 46
BsiHKAI G_wGCw'C
Cuts at: 958 1163 2069 2359 3137 3327 4187 958
Size: 205 906 290 778 190 860 2116
Fragments arranged by size:
2116 906 860 778 290 205 190
Bsp1286I G_dGCh'C
Cuts at: 680 764 874 958 1024 1163 1276 1877 1941
Size: 84 110 84 66 139 113 601 64
Cuts at: 1941 1997 2069 2242 2359 2970 3063 3137 3327
Size: 56 72 173 117 611 93 74 190
Cuts at: 3327 3389 4187 680
Size: 62 798 1838
Fragments arranged by size:
1838 798 611 601 190 173 139 117
113 110 93 84 84 74 72 66
64 62 56
BspHI T'CATG_A
Cuts at: 3777 3777
Size: 5345
BspMI ACCTGCnnnn'nnnn_
Cuts at: 886 1001 1691 2395 2609 2911 3292 886
Size: 115 690 704 214 302 381 2939
Fragments arranged by size:
2939 704 690 381 302 214 115
BsrBI CCG'CTC
Cuts at: 50 1788 3637 3691 5175 5340 50
Size: 1738 1849 54 1484 165 55
Fragments arranged by size:
1849 1738 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3257 3257
Size: 5345
BsrFI r'CCGG_y
Cuts at: 704 1843 1911 2188 3343 3524 704
Size: 1139 68 277 1155 181 2525
Fragments arranged by size:
2525 1155 1139 277 181 68
BssSI C'ACGA_G
Cuts at: 1957 3616 4324 1957
Size: 1659 708 2978
Fragments arranged by size:
2978 1659 708
BstAPI GCAn_nnn'nTGC
Cuts at: 962 1952 2722 962
Size: 990 770 3585
Fragments arranged by size:
3585 990 770
BstBI TT'CG_AA
Cuts at: 320 3706 320
Size: 3386 1959
BstEII G'GTnAC_C
Cuts at: 912 2149 912
Size: 1237 4108
BstYI r'GATC_y
Cuts at: 1 2046 2370 2837 2860 3195 3441 3747 3759
Size: 2045 324 467 23 335 246 306 12
Cuts at: 3759 3845 4897 1
Size: 86 1052 449
Fragments arranged by size:
2045 1052 467 449 335 324 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1852 1852
Size: 5345
BtgI C'CryG_G
Cuts at: 426 1137 426
Size: 711 4634
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3295 3476 3639 3295
Size: 181 163 5001
Fragments arranged by size:
5001 181 163
BtsI GCAGTG_nn'
Cuts at: 186 874 960 1950 2330 2588 186
Size: 688 86 990 380 258 2943
Fragments arranged by size:
2943 990 688 380 258 86
ClaI AT'CG_AT
Cuts at: 821 821
Size: 5345
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1617 1652 1617
Size: 35 5310
DraI TTT'AAA
Cuts at: 2344 2344
Size: 5345
DrdI GACnn_nn'nnGTC
Cuts at: 3051 4395 3051
Size: 1344 4001
EaeI y'GGCC_r
Cuts at: 1134 1490 2169 2930 3104 3495 3522 4867 1134
Size: 356 679 761 174 391 27 1345 1612
Fragments arranged by size:
1612 1345 761 679 391 356 174 27
EagI C'GGCC_G
Cuts at: 1134 2930 1134
Size: 1796 3549
EarI CTCTTCn'nnn_
Cuts at: 340 979 1243 1961 2131 3368 3578 340
Size: 639 264 718 170 1237 210 2107
Fragments arranged by size:
2107 1237 718 639 264 210 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3553 4295 4441 5155 3553
Size: 742 146 714 3743
Fragments arranged by size:
3743 742 714 146
EcoICRI GAG'CTC
Cuts at: 1161 2357 1161
Size: 1196 4149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 684 1099 1267 1759 1882 2104 2320 3170 3602
Size: 415 168 492 123 222 216 850 432
Cuts at: 3602 3955 4617 684
Size: 353 662 1412
Fragments arranged by size:
1412 850 662 492 432 415 353 222
216 168 123
EcoNI CCTnn'n_nnAGG
Cuts at: 287 287
Size: 5345
EcoO109I rG'GnC_Cy
Cuts at: 1272 1273 1791 2238 2239 2484 1272
Size: 1 518 447 1 245 4133
Fragments arranged by size:
4133 518 447 245 1 1
EcoRI G'AATT_C
Cuts at: 1645 2349 1645
Size: 704 4641
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2344 3364 2344
Size: 1020 4325
FspI TGC'GCA
Cuts at: 3126 3126
Size: 5345
HaeII r_GCGC'y
Cuts at: 584 864 1086 1899 2779 3027 4257 5028 584
Size: 280 222 813 880 248 1230 771 901
Fragments arranged by size:
1230 901 880 813 771 280 248 222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2035 2067 2101 2133 3187 3217 3219 3249 2035
Size: 32 34 32 1054 30 2 30 4131
Fragments arranged by size:
4131 1054 34 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2384 2384
Size: 5345
HindIII A'AGCT_T
Cuts at: 1630 1630
Size: 5345
KasI G'GCGC_C
Cuts at: 2775 3023 2775
Size: 248 5097
KpnI G_GTAC'C
Cuts at: 2365 2365
Size: 5345
MluI A'CGCG_T
Cuts at: 950 4778 950
Size: 3828 1517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2042 4104 4288 2042
Size: 2062 184 3099
Fragments arranged by size:
3099 2062 184
MscI TGG'CCA
Cuts at: 1492 3106 1492
Size: 1614 3731
MslI CAynn'nnrTG
Cuts at: 361 1187 1207 1439 1457 2276 3334 3461 361
Size: 826 20 232 18 819 1058 127 2245
Fragments arranged by size:
2245 1058 826 819 232 127 20 18
MspA1I CmG'CkG
Cuts at: 1139 1245 1329 2456 2770 3130 3914 4159 1139
Size: 106 84 1127 314 360 784 245 2325
Fragments arranged by size:
2325 1127 784 360 314 245 106 84
NaeI GCC'GGC
Cuts at: 706 2190 3526 706
Size: 1484 1336 2525
Fragments arranged by size:
2525 1484 1336
NarI GG'CG_CC
Cuts at: 2776 3024 2776
Size: 248 5097
NgoMIV G'CCGG_C
Cuts at: 704 2188 3524 704
Size: 1484 1336 2525
Fragments arranged by size:
2525 1484 1336
NotI GC'GGCC_GC
Cuts at: 1134 1134
Size: 5345
NsiI A_TGCA'T
Cuts at: 2118 2118
Size: 5345
NspI r_CATG'y
Cuts at: 354 1008 1680 2398 2898 4501 354
Size: 654 672 718 500 1603 1198
Fragments arranged by size:
1603 1198 718 672 654 500
PasI CC'CwG_GG
Cuts at: 1941 1941
Size: 5345
PciI A'CATG_T
Cuts at: 4497 4497
Size: 5345
PflMI CCAn_nnn'nTGG
Cuts at: 924 1809 924
Size: 885 4460
PfoI T'CCnGG_A
Cuts at: 239 239
Size: 5345
PmeI GTTT'AAAC
Cuts at: 2344 2344
Size: 5345
PmlI CAC'GTG
Cuts at: 1509 1509
Size: 5345
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 3791
Size: 32 5313
PspOMI G'GGCC_C
Cuts at: 1272 2238 1272
Size: 966 4379
PstI C_TGCA'G
Cuts at: 197 384 1334 2392 3077 197
Size: 187 950 1058 685 2465
Fragments arranged by size:
2465 1058 950 685 187
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5345
PvuII CAG'CTG
Cuts at: 1245 1329 2770 3130 1245
Size: 84 1441 360 3460
Fragments arranged by size:
3460 1441 360 84
RsrII CG'GwC_CG
Cuts at: 3540 3540
Size: 5345
SacI G_AGCT'C
Cuts at: 1163 2359 1163
Size: 1196 4149
SacII CC_GC'GG
Cuts at: 1140 1140
Size: 5345
SalI G'TCGA_C
Cuts at: 2382 2382
Size: 5345
SapI GCTCTTCn'nnn_
Cuts at: 340 979 3368 3578 340
Size: 639 2389 210 2107
Fragments arranged by size:
2389 2107 639 210
SbfI CC_TGCA'GG
Cuts at: 2392 2392
Size: 5345
ScaI AGT'ACT
Cuts at: 4862 4862
Size: 5345
SexAI A'CCwGG_T
Cuts at: 908 908
Size: 5345
SfcI C'TryA_G
Cuts at: 33 193 380 1330 1998 2388 3073 4041 4232
Size: 160 187 950 668 390 685 968 191
Cuts at: 4232 33
Size: 1146
Fragments arranged by size:
1146 968 950 685 668 390 191 187
160
SfoI GGC'GCC
Cuts at: 2777 3025 2777
Size: 248 5097
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5345
SmaI CCC'GGG
Cuts at: 312 653 2367 312
Size: 341 1714 3290
Fragments arranged by size:
3290 1714 341
SmlI C'TyrA_G
Cuts at: 1162 1414 1582 2500 3415 4129 4391 1162
Size: 252 168 918 915 714 262 2116
Fragments arranged by size:
2116 918 915 714 262 252 168
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5345
SphI G_CATG'C
Cuts at: 354 2398 2898 354
Size: 2044 500 2801
Fragments arranged by size:
2801 2044 500
StuI AGG'CCT
Cuts at: 1057 1057
Size: 5345
StyI C'CwwG_G
Cuts at: 1235 1803 1904 2120 2154 2479 2833 1235
Size: 568 101 216 34 325 354 3747
Fragments arranged by size:
3747 568 354 325 216 101 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 931 1078 1447 2928 3061 3520 3729 931
Size: 147 369 1481 133 459 209 2547
Fragments arranged by size:
2547 1481 459 369 209 147 133
TatI w'GTAC_w
Cuts at: 902 1283 4860 902
Size: 381 3577 1387
Fragments arranged by size:
3577 1387 381
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1441 1473 2133 2165 4862 4894 1441
Size: 32 660 32 2697 32 1892
Fragments arranged by size:
2697 1892 660 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3142 3142
Size: 5345
XbaI T'CTAG_A
Cuts at: 2376 2376
Size: 5345
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 789 906 2840 789
Size: 117 1934 3294
Fragments arranged by size:
3294 1934 117
XmaI C'CCGG_G
Cuts at: 310 651 2365 310
Size: 341 1714 3290
Fragments arranged by size:
3290 1714 341
XmnI GAAnn'nnTTC
Cuts at: 973 1618 1645 973
Size: 645 27 4673
Fragments arranged by size:
4673 645 27
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AlfI AloI AscI AvrII
BaeI BbvCI BglI BmtI Bpu10I BsiWI BsmI BsmBI
BspEI BsrGI BssHII BstXI BstZ17I DraIII EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NruI PacI
PpuMI PshAI PsiI PspXI PsrI SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI