Restriction map for pF1KB8422
 (Linear) (Six-base) MAPSORT of: KIBB8422.seq  Check: 3524  from: 1  to: 2232



KIBB8422.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     778     893    1583    2232 
   Size:        778     115     690     649

  Fragments arranged by size: 

                 778     690     649     115
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     576     991    1159    1651    2232 
   Size:        576     415     168     492     581

  Fragments arranged by size: 

                 581     576     492     415     168
 
AfeI AGC'GCT

Cuts at:      0     754    2232 
   Size:        754    1478
 
AflIII A'CryG_T

Cuts at:      0     842    1398    2232 
   Size:        842     556     834

  Fragments arranged by size: 

                 842     834     556
 
AleI CACnn'nnGTG

Cuts at:      0    1079    1331    2232 
   Size:       1079     252     901

  Fragments arranged by size: 

                1079     901     252
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1844    2232 
   Size:       1844     388
 
ApaI G_GGCC'C

Cuts at:      0    1168    2134    2232 
   Size:       1168     966      98

  Fragments arranged by size: 

                1168     966      98
 
ApaLI G'TGCA_C

Cuts at:      0     846    2232 
   Size:        846    1386
 
ApoI r'AATT_y

Cuts at:      0    1537    1621    2232 
   Size:       1537      84     611

  Fragments arranged by size: 

                1537     611      84
 
AvaI C'yCGr_G

Cuts at:      0     101     202     543    1212    1933    1966    2232 
   Size:        101     101     341     669     721      33     266

  Fragments arranged by size: 

                 721     669     341     266     101     101      33
 
BanI G'GyrC_C

Cuts at:      0    1800    2232 
   Size:       1800     432
 
BanII G_rGCy'C

Cuts at:      0     656    1055    1168    1769    1889    2134    2232 
   Size:        656     399     113     601     120     245      98

  Fragments arranged by size: 

                 656     601     399     245     120     113      98
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      67     201    2232 
   Size:         67     134    2031

  Fragments arranged by size: 

                2031     134      67
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1203    1237    2232 
   Size:       1203      34     995

  Fragments arranged by size: 

                1203     995      34
 
BclI T'GATC_A

Cuts at:      0      20    2232 
   Size:         20    2212
 
BfrBI ATG'CAT

Cuts at:      0    2008    2232 
   Size:       2008     224
 
BlpI GC'TnA_GC

Cuts at:      0     877    2232 
   Size:        877    1355
 
Bme1580I G_kGCm'C

Cuts at:      0     572     766     850     916    1168    1833    2134    2232 
   Size:        572     194      84      66     252     665     301      98

  Fragments arranged by size: 

                 665     572     301     252     194      98      84      66
            
 
BmgBI CAC'GTC

Cuts at:      0     699     735    1503    2232 
   Size:        699      36     768     729

  Fragments arranged by size: 

                 768     729     699      36
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     153    2232 
   Size:        153    2079
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1774    1996    2212    2232 
   Size:       1774     222     216      20

  Fragments arranged by size: 

                1774     222     216      20
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1039    1291    1459    2232 
   Size:       1039     252     168     773

  Fragments arranged by size: 

                1039     773     252     168
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     179     664    2232 
   Size:        179     485    1568

  Fragments arranged by size: 

                1568     485     179
 
BsaAI yAC'GTr

Cuts at:      0     729    1401    2232 
   Size:        729     672     831

  Fragments arranged by size: 

                 831     729     672
 
BsaWI w'CCGG_w

Cuts at:      0    1544    2232 
   Size:       1544     688
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     483     513    1168    1198    1333    1363    2025    2055 
   Size:        483      30     655      30     135      30     662      30

Cuts at:   2055    2232 
   Size:        177

  Fragments arranged by size: 

                 662     655     483     177     135      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     319     682    1761    1777    1867    1876    2008    2046 
   Size:        319     363    1079      16      90       9     132      38

Cuts at:   2046    2232 
   Size:        186

  Fragments arranged by size: 

                1079     363     319     186     132      90      38      16
                   9
 
BseYI C'CCAG_C

Cuts at:      0     469     485    1045    1133    2232 
   Size:        469      16     560      88    1099

  Fragments arranged by size: 

                1099     560     469      88      16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     727    1312    1480    1561    1911    2232 
   Size:        727     585     168      81     350     321

  Fragments arranged by size: 

                 727     585     350     321     168      81
 
BsiEI CG_ry'CG

Cuts at:      0    1029    2232 
   Size:       1029    1203
 
BsiHKAI G_wGCw'C

Cuts at:      0     850    1055    1961    2232 
   Size:        850     205     906     271

  Fragments arranged by size: 

                 906     850     271     205
 
Bsp1286I G_dGCh'C

Cuts at:      0     572     656     766     850     916    1055    1168    1769 
   Size:        572      84     110      84      66     139     113     601

Cuts at:   1769    1833    1889    1961    2134    2232 
   Size:         64      56      72     173      98

  Fragments arranged by size: 

                 601     572     173     139     113     110      98      84
                  84      72      66      64      56
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     778     893    1583    2232 
   Size:        778     115     690     649

  Fragments arranged by size: 

                 778     690     649     115
 
BsrBI CCG'CTC

Cuts at:      0    1680    2232 
   Size:       1680     552
 
BsrFI r'CCGG_y

Cuts at:      0     596    1735    1803    2080    2232 
   Size:        596    1139      68     277     152

  Fragments arranged by size: 

                1139     596     277     152      68
 
BssSI C'ACGA_G

Cuts at:      0    1849    2232 
   Size:       1849     383
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     854    1844    2232 
   Size:        854     990     388

  Fragments arranged by size: 

                 990     854     388
 
BstBI TT'CG_AA

Cuts at:      0     212    2232 
   Size:        212    2020
 
BstEII G'GTnAC_C

Cuts at:      0     804    2041    2232 
   Size:        804    1237     191

  Fragments arranged by size: 

                1237     804     191
 
BstYI r'GATC_y

Cuts at:      0    1938    2232 
   Size:       1938     294
 
Bsu36I CC'TnA_GG

Cuts at:      0    1744    2232 
   Size:       1744     488
 
BtgI C'CryG_G

Cuts at:      0     318    1029    2232 
   Size:        318     711    1203

  Fragments arranged by size: 

                1203     711     318
 
BtsI GCAGTG_nn'

Cuts at:      0      78     766     852    1842    2222    2232 
   Size:         78     688      86     990     380      10

  Fragments arranged by size: 

                 990     688     380      86      78      10
 
ClaI AT'CG_AT

Cuts at:      0     713    2232 
   Size:        713    1519
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1509    1544    2232 
   Size:       1509      35     688

  Fragments arranged by size: 

                1509     688      35
 
EaeI y'GGCC_r

Cuts at:      0    1026    1382    2061    2232 
   Size:       1026     356     679     171

  Fragments arranged by size: 

                1026     679     356     171
 
EagI C'GGCC_G

Cuts at:      0    1026    2232 
   Size:       1026    1206
 
EarI CTCTTCn'nnn_

Cuts at:      0     232     871    1135    1853    2023    2232 
   Size:        232     639     264     718     170     209

  Fragments arranged by size: 

                 718     639     264     232     209     170
 
EcoICRI GAG'CTC

Cuts at:      0    1053    2232 
   Size:       1053    1179
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     576     991    1159    1651    1774    1996    2212    2232 
   Size:        576     415     168     492     123     222     216      20

  Fragments arranged by size: 

                 576     492     415     222     216     168     123      20
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     179    2232 
   Size:        179    2053
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1164    1165    1683    2130    2131    2232 
   Size:       1164       1     518     447       1     101

  Fragments arranged by size: 

                1164     518     447     101       1       1
 
EcoRI G'AATT_C

Cuts at:      0    1537    2232 
   Size:       1537     695
 
HaeII r_GCGC'y

Cuts at:      0     476     756     978    1791    2232 
   Size:        476     280     222     813     441

  Fragments arranged by size: 

                 813     476     441     280     222
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1927    1959    1993    2025    2232 
   Size:       1927      32      34      32     207

  Fragments arranged by size: 

                1927     207      34      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1522    2232 
   Size:       1522     710
 
MluI A'CGCG_T

Cuts at:      0     842    2232 
   Size:        842    1390
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1934    2232 
   Size:       1934     298
 
MscI TGG'CCA

Cuts at:      0    1384    2232 
   Size:       1384     848
 
MslI CAynn'nnrTG

Cuts at:      0     253    1079    1099    1331    1349    2168    2232 
   Size:        253     826      20     232      18     819      64

  Fragments arranged by size: 

                 826     819     253     232      64      20      18
 
MspA1I CmG'CkG

Cuts at:      0    1031    1137    1221    2232 
   Size:       1031     106      84    1011

  Fragments arranged by size: 

                1031    1011     106      84
 
NaeI GCC'GGC

Cuts at:      0     598    2082    2232 
   Size:        598    1484     150

  Fragments arranged by size: 

                1484     598     150
 
NgoMIV G'CCGG_C

Cuts at:      0     596    2080    2232 
   Size:        596    1484     152

  Fragments arranged by size: 

                1484     596     152
 
NotI GC'GGCC_GC

Cuts at:      0    1026    2232 
   Size:       1026    1206
 
NsiI A_TGCA'T

Cuts at:      0    2010    2232 
   Size:       2010     222
 
NspI r_CATG'y

Cuts at:      0     246     900    1572    2232 
   Size:        246     654     672     660

  Fragments arranged by size: 

                 672     660     654     246
 
PasI CC'CwG_GG

Cuts at:      0    1833    2232 
   Size:       1833     399
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     816    1701    2232 
   Size:        816     885     531

  Fragments arranged by size: 

                 885     816     531
 
PfoI T'CCnGG_A

Cuts at:      0     131    2232 
   Size:        131    2101
 
PmlI CAC'GTG

Cuts at:      0    1401    2232 
   Size:       1401     831
 
PspOMI G'GGCC_C

Cuts at:      0    1164    2130    2232 
   Size:       1164     966     102

  Fragments arranged by size: 

                1164     966     102
 
PstI C_TGCA'G

Cuts at:      0      89     276    1226    2232 
   Size:         89     187     950    1006

  Fragments arranged by size: 

                1006     950     187      89
 
PvuII CAG'CTG

Cuts at:      0    1137    1221    2232 
   Size:       1137      84    1011

  Fragments arranged by size: 

                1137    1011      84
 
SacI G_AGCT'C

Cuts at:      0    1055    2232 
   Size:       1055    1177
 
SacII CC_GC'GG

Cuts at:      0    1032    2232 
   Size:       1032    1200
 
SapI GCTCTTCn'nnn_

Cuts at:      0     232     871    2232 
   Size:        232     639    1361

  Fragments arranged by size: 

                1361     639     232
 
SexAI A'CCwGG_T

Cuts at:      0     800    2232 
   Size:        800    1432
 
SfcI C'TryA_G

Cuts at:      0      85     272    1222    1890    2232 
   Size:         85     187     950     668     342

  Fragments arranged by size: 

                 950     668     342     187      85
 
SmaI CCC'GGG

Cuts at:      0     204     545    2232 
   Size:        204     341    1687

  Fragments arranged by size: 

                1687     341     204
 
SmlI C'TyrA_G

Cuts at:      0    1054    1306    1474    2232 
   Size:       1054     252     168     758

  Fragments arranged by size: 

                1054     758     252     168
 
SphI G_CATG'C

Cuts at:      0     246    2232 
   Size:        246    1986
 
StuI AGG'CCT

Cuts at:      0     949    2232 
   Size:        949    1283
 
StyI C'CwwG_G

Cuts at:      0    1127    1695    1796    2012    2046    2232 
   Size:       1127     568     101     216      34     186

  Fragments arranged by size: 

                1127     568     216     186     101      34
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     823     970    1339    2232 
   Size:        823     147     369     893

  Fragments arranged by size: 

                 893     823     369     147
 
TatI w'GTAC_w

Cuts at:      0     794    1175    2232 
   Size:        794     381    1057

  Fragments arranged by size: 

                1057     794     381
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1333    1365    2025    2057    2232 
   Size:       1333      32     660      32     175

  Fragments arranged by size: 

                1333     660     175      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     681     798    2232 
   Size:        681     117    1434

  Fragments arranged by size: 

                1434     681     117
 
XmaI C'CCGG_G

Cuts at:      0     202     543    2232 
   Size:        202     341    1689

  Fragments arranged by size: 

                1689     341     202
 
XmnI GAAnn'nnTTC

Cuts at:      0     865    1510    1537    2232 
   Size:        865     645      27     695

  Fragments arranged by size: 

                 865     695     645      27
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AleI    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BanI    BanII     BbsI     BcgI     BclI    BfrBI
     BlpI Bme1580I    BmgBI     BmrI     BpmI    BpuEI     BsaI    BsaAI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspMI    BsrBI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstYI
   Bsu36I     BtgI     BtsI     ClaI    CspCI     EaeI     EagI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I  HindIII
     MluI     MmeI     MscI     MslI   MspA1I     NaeI   NgoMIV     NotI
     NsiI     NspI     PasI    PflMI     PfoI     PmlI   PspOMI     PstI
    PvuII     SacI    SacII     SapI    SexAI     SfcI     SmaI     SmlI
     SphI     StuI     StyI    TaqII     TatI     TstI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AlfI
     AloI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
    BbvCI    BciVI     BglI    BglII     BmtI     BplI   Bpu10I    BsaBI
    BsaHI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI
   BssHII    BstXI  BstZ17I    BtgZI     DraI   DraIII     DrdI     EciI
    EcoRV     FalI     FseI     FspI    FspAI   HincII     HpaI     KasI
     KpnI     MfeI     NarI     NcoI     NdeI     NheI     NruI     PacI
     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SalI    SanDI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI  Tth111I
     XbaI     XhoI     ZraI
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