Restriction map for
pF1KB8419
(Circular) (Six-base) MAPSORT of: pF1KB8419.seq Check: 9531 from: 1 to: 4700
pF1KB8419 4700 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 491 1738 491
Size: 1247 3453
Acc65I G'GTAC_C
Cuts at: 1716 1716
Size: 4700
AclI AA'CG_TT
Cuts at: 4540 4540
Size: 4700
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 513 681 849 1437 1521 2525 2957 3310
Size: 268 168 168 588 84 1004 432 353
Cuts at: 3310 3972 245
Size: 662 973
Fragments arranged by size:
1004 973 662 588 432 353 268 168
168 84
AflIII A'CryG_T
Cuts at: 2052 3852 4133 2052
Size: 1800 281 2619
Fragments arranged by size:
2619 1800 281
AleI CACnn'nnGTG
Cuts at: 992 1387 992
Size: 395 4305
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 323 323
Size: 4700
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1626 1658 1626
Size: 32 4668
AlwNI CAG_nnn'CTG
Cuts at: 2005 3443 2005
Size: 1438 3262
ApaLI G'TGCA_C
Cuts at: 3538 3538
Size: 4700
ApoI r'AATT_y
Cuts at: 396 477 1113 1571 1683 1704 4053 4309 396
Size: 81 636 458 112 21 2349 256 787
Fragments arranged by size:
2349 787 636 458 256 112 81 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 4700
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
AvaI C'yCGr_G
Cuts at: 763 1720 763
Size: 957 3743
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1500 1533 1500
Size: 33 4667
BamHI G'GATC_C
Cuts at: 1725 1725
Size: 4700
BanI G'GyrC_C
Cuts at: 1478 1716 2130 2378 2413 1478
Size: 238 414 248 35 3765
Fragments arranged by size:
3765 414 248 238 35
BanII G_rGCy'C
Cuts at: 1714 2744 1714
Size: 1030 3670
BbeI G_GCGC'C
Cuts at: 2134 2382 2134
Size: 248 4452
BciVI GTATCCnnnnn_n'
Cuts at: 2598 3654 4700 2598
Size: 1056 1046 2598
Fragments arranged by size:
2598 1056 1046
BclI T'GATC_A
Cuts at: 2220 2220
Size: 4700
BglII A'GATC_T
Cuts at: 2215 4252 2215
Size: 2037 2663
BlpI GC'TnA_GC
Cuts at: 1812 1812
Size: 4700
Bme1580I G_kGCm'C
Cuts at: 931 2325 2418 3542 931
Size: 1394 93 1124 2089
Fragments arranged by size:
2089 1394 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1912 1993 2328 4472 1912
Size: 81 335 2144 2140
Fragments arranged by size:
2144 2140 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3067 3067
Size: 4700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 929 929
Size: 4700
Bpu10I CC'TnA_GC
Cuts at: 1291 1291
Size: 4700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1036 1876 2755 3469 3767 1036
Size: 840 879 714 298 1969
Fragments arranged by size:
1969 879 840 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 138 4404 138
Size: 4266 434
BsaAI yAC'GTr
Cuts at: 1578 2053 1578
Size: 475 4225
BsaBI GATnn'nnATC
Cuts at: 6 2214 4251 6
Size: 2208 2037 455
Fragments arranged by size:
2208 2037 455
BsaHI Gr'CG_yC
Cuts at: 2131 2379 3081 4577 2131
Size: 248 702 1496 2254
Fragments arranged by size:
2254 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1481 1889 2410 3088 3499 3646 3920 1481
Size: 408 521 678 411 147 274 2261
Fragments arranged by size:
2261 678 521 411 408 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 427 427
Size: 4700
BseYI C'CCAG_C
Cuts at: 2125 2883 3548 3956 2125
Size: 758 665 408 2869
Fragments arranged by size:
2869 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2242 2288 3518 104
Size: 2138 46 1230 1286
Fragments arranged by size:
2138 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1714 2492 2682 3542 1714
Size: 778 190 860 2872
Fragments arranged by size:
2872 860 778 190
BsmI GAATG_Cn'
Cuts at: 653 1234 653
Size: 581 4119
BsmBI CGTCTCn'nnnn_
Cuts at: 1683 1683
Size: 4700
Bsp1286I G_dGCh'C
Cuts at: 931 1714 2325 2418 2492 2682 2744 3542 931
Size: 783 611 93 74 190 62 798 2089
Fragments arranged by size:
2089 798 783 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 354 1620 3132 354
Size: 1266 1512 1922
Fragments arranged by size:
1922 1512 1266
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1750 1964 2266 2647 1750
Size: 214 302 381 3803
Fragments arranged by size:
3803 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2992 3046 4530 4695 50
Size: 2942 54 1484 165 55
Fragments arranged by size:
2942 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1221 2612 1221
Size: 1391 3309
BsrFI r'CCGG_y
Cuts at: 2698 2879 2698
Size: 181 4519
BssSI C'ACGA_G
Cuts at: 2971 3679 2971
Size: 708 3992
BstAPI GCAn_nnn'nTGC
Cuts at: 2077 2077
Size: 4700
BstBI TT'CG_AA
Cuts at: 3061 3061
Size: 4700
BstXI CCAn_nnnn'nTGG
Cuts at: 329 329
Size: 4700
BstYI r'GATC_y
Cuts at: 1 1725 2192 2215 2550 2796 3102 3114 3200
Size: 1724 467 23 335 246 306 12 86
Cuts at: 3200 4252 1
Size: 1052 449
Fragments arranged by size:
1724 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4700
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2650 2831 2994 2650
Size: 181 163 4356
Fragments arranged by size:
4356 181 163
BtsI GCAGTG_nn'
Cuts at: 1943 1943
Size: 4700
DraI TTT'AAA
Cuts at: 1699 1699
Size: 4700
DrdI GACnn_nn'nnGTC
Cuts at: 2406 3750 2406
Size: 1344 3356
EaeI y'GGCC_r
Cuts at: 2285 2459 2850 2877 4222 2285
Size: 174 391 27 1345 2763
Fragments arranged by size:
2763 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2285 2285
Size: 4700
EarI CTCTTCn'nnn_
Cuts at: 769 937 1273 1671 2723 2933 769
Size: 168 336 398 1052 210 2536
Fragments arranged by size:
2536 1052 398 336 210 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2908 3650 3796 4510 2908
Size: 742 146 714 3098
Fragments arranged by size:
3098 742 714 146
EcoICRI GAG'CTC
Cuts at: 1712 1712
Size: 4700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 513 681 849 929 1437 1521 2525 2957
Size: 268 168 168 80 508 84 1004 432
Cuts at: 2957 3310 3972 245
Size: 353 662 973
Fragments arranged by size:
1004 973 662 508 432 353 268 168
168 84 80
EcoNI CCTnn'n_nnAGG
Cuts at: 182 182
Size: 4700
EcoO109I rG'GnC_Cy
Cuts at: 563 1487 1839 563
Size: 924 352 3424
Fragments arranged by size:
3424 924 352
EcoRI G'AATT_C
Cuts at: 477 1704 477
Size: 1227 3473
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2719 2719
Size: 4700
FspI TGC'GCA
Cuts at: 2481 2481
Size: 4700
HaeII r_GCGC'y
Cuts at: 2134 2382 3612 4383 2134
Size: 248 1230 771 2451
Fragments arranged by size:
2451 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 336 364 368 396 624 656 936 968 2542
Size: 28 4 28 228 32 280 32 1574
Cuts at: 2542 2572 2574 2604 336
Size: 30 2 30 2432
Fragments arranged by size:
2432 1574 280 228 32 32 30 30
28 28 4 2
HincII GTy'rAC
Cuts at: 1739 1739
Size: 4700
HindIII A'AGCT_T
Cuts at: 525 693 1029 1449 525
Size: 168 336 420 3776
Fragments arranged by size:
3776 420 336 168
KasI G'GCGC_C
Cuts at: 2130 2378 2130
Size: 248 4452
KpnI G_GTAC'C
Cuts at: 1720 1720
Size: 4700
MluI A'CGCG_T
Cuts at: 4133 4133
Size: 4700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 499 3459 3643 499
Size: 2960 184 1556
Fragments arranged by size:
2960 1556 184
MscI TGG'CCA
Cuts at: 2461 2461
Size: 4700
MslI CAynn'nnrTG
Cuts at: 305 327 458 824 840 875 992 1260 1387
Size: 22 131 366 16 35 117 268 127
Cuts at: 1387 2689 2816 305
Size: 1302 127 2189
Fragments arranged by size:
2189 1302 366 268 131 127 127 117
35 22 16
MspA1I CmG'CkG
Cuts at: 981 1523 1811 2125 2485 3269 3514 981
Size: 542 288 314 360 784 245 2167
Fragments arranged by size:
2167 784 542 360 314 288 245
NaeI GCC'GGC
Cuts at: 2881 2881
Size: 4700
NarI GG'CG_CC
Cuts at: 2131 2379 2131
Size: 248 4452
NcoI C'CATG_G
Cuts at: 107 107
Size: 4700
NdeI CA'TA_TG
Cuts at: 837 837
Size: 4700
NgoMIV G'CCGG_C
Cuts at: 2879 2879
Size: 4700
NspI r_CATG'y
Cuts at: 1753 2253 3856 1753
Size: 500 1603 2597
Fragments arranged by size:
2597 1603 500
PciI A'CATG_T
Cuts at: 3852 3852
Size: 4700
PflMI CCAn_nnn'nTGG
Cuts at: 1105 1105
Size: 4700
PmeI GTTT'AAAC
Cuts at: 1699 1699
Size: 4700
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 4668
PpuMI rG'GwC_Cy
Cuts at: 563 1487 563
Size: 924 3776
PsiI TTA'TAA
Cuts at: 296 587 923 1427 296
Size: 291 336 504 3569
Fragments arranged by size:
3569 504 336 291
PstI C_TGCA'G
Cuts at: 1747 2432 1747
Size: 685 4015
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4700
PvuII CAG'CTG
Cuts at: 1523 2125 2485 1523
Size: 602 360 3738
Fragments arranged by size:
3738 602 360
RsrII CG'GwC_CG
Cuts at: 2895 2895
Size: 4700
SacI G_AGCT'C
Cuts at: 1714 1714
Size: 4700
SalI G'TCGA_C
Cuts at: 1737 1737
Size: 4700
SapI GCTCTTCn'nnn_
Cuts at: 2723 2933 2723
Size: 210 4490
SbfI CC_TGCA'GG
Cuts at: 1747 1747
Size: 4700
ScaI AGT'ACT
Cuts at: 4217 4217
Size: 4700
SfcI C'TryA_G
Cuts at: 33 1743 2428 3396 3587 33
Size: 1710 685 968 191 1146
Fragments arranged by size:
1710 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2132 2380 2132
Size: 248 4452
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
SmaI CCC'GGG
Cuts at: 1722 1722
Size: 4700
SmlI C'TyrA_G
Cuts at: 1015 1855 2770 3484 3746 1015
Size: 840 915 714 262 1969
Fragments arranged by size:
1969 915 840 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4700
SphI G_CATG'C
Cuts at: 1753 2253 1753
Size: 500 4200
StyI C'CwwG_G
Cuts at: 107 1834 2188 107
Size: 1727 354 2619
Fragments arranged by size:
2619 1727 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2283 2416 2875 3084 2283
Size: 133 459 209 3899
Fragments arranged by size:
3899 459 209 133
TatI w'GTAC_w
Cuts at: 4215 4215
Size: 4700
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4217 4249 4217
Size: 32 4668
Tth111I GACn'n_nGTC
Cuts at: 2497 2497
Size: 4700
XbaI T'CTAG_A
Cuts at: 1731 1731
Size: 4700
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2195 2195
Size: 4700
XmaI C'CCGG_G
Cuts at: 1720 1720
Size: 4700
XmnI GAAnn'nnTTC
Cuts at: 215 526 694 778 1366 1534 215
Size: 311 168 84 588 168 3381
Fragments arranged by size:
3381 588 311 168 168 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI ApaI AscI
AvrII BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsgI BsiWI BspEI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI