Restriction map for pF1KB8411
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
ApaLI       AscI        Asp718I     AspEI       AvrII       
BamHI       BbeI        BcgI        BciVI       BcnI        
BfrI        BglII       BlnI        BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstBI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EclXI       Eco52I      EcoNI       EcoRV       EcoT22I     
FseI        Hin1I       HpaI        KasI        KpnI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmeI        Psp1406I    PvuI        RcaI        RsrII       
SalI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SphI        Sse8387I    
SspBI       SspI        SwaI        Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AflIII       1693  ACGTGT               A^C[GA][CT]GT
AocI          580  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      1103  AGCGCT               AGC^GCT
AseI         2119  ATTAAT               AT^TAAT
AsnI         2119  ATTAAT               AT^TAAT
AvaI         2352  CCCGAG               C^[CT]CG[GA]G
AviII        1588  TGCGCA               TGC^GCA
BbrPI        1692  CACGTG               CAC^GTG
BclI          489  TGATCA               T^GATCA
BglI         1633  GCCCAAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1668  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1668  GCTCAGC              GC^T[ACGT]AGC
BsiWI        2486  CGTACG               C^GTACG
BsoBI        2352  CCCGAG               C^[CT]CG[GA]G
BsrBI        2151  CCGCTC               CCGCTC(-3/-3)
BssSI        1183  CACGAG               CACGAG(-5/-1)
BstEII        138  GGTCACC              G^GT[ACGT]ACC
BstPI         138  GGTCACC              G^GT[ACGT]ACC
Bsu36I        580  CCTCAGG              CC^T[ACGT]AGG
CelII        1668  GCTCAGC              GC^T[ACGT]AGC
DrdI         1798  GACGAGCTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         2584  CCGCGG               C^C[GA][CT]GG
Eco47III     1103  AGCGCT               AGC^GCT
Eco81I        580  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       138  GGTCACC              G^GT[ACGT]ACC
EcoT14I       652  CCAAGG               C^C[AT][AT]GG
FbaI          489  TGATCA               T^GATCA
FspI         1588  TGCGCA               TGC^GCA
HaeII        1103  AGCGCT               [GA]GCGC^[CT]
HgaI         1581  GACGC                GACGC(5/10)
HindIII      2224  AAGCTT               A^AGCTT
KspI         2584  CCGCGG               CCGC^GG
PmaCI        1692  CACGTG               CAC^GTG
PmlI         1692  CACGTG               CAC^GTG
PshAI        2524  GACTTTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        2119  ATTAAT               AT^TAAT
PstI          827  CTGCAG               CTGCA^G
SacII        2584  CCGCGG               CCGC^GG
ScaI         1751  AGTACT               AGT^ACT
SexAI         569  ACCAGGT              A^CC[AT]GGT
SmlI          788  CTCAAG               C^T[CT][GA]AG
SpeI          858  ACTAGT               A^CTAGT
StuI         1217  AGGCCT               AGG^CCT
StyI          652  CCAAGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccII        2406  CGCG                 CG^CG
             2585  CGCG                
AspI          305  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              331  GACATTGTC           
BanI         1811  GGTGCC               G^G[CT][GA]CC
             2086  GGTGCC              
BbvCI         151  CCTCAGC              CCTCAGC(-5/-2)
              697  CCTCAGC             
BsaAI        1692  CACGTG               [CT]AC^GT[GA]
             2488  TACGTG              
BspMI        2372  ACCTGC               ACCTGC(4/8)
             2558  ACCTGC              
BstUI        2406  CGCG                 CG^CG
             2585  CGCG                
BstYI         234  GGATCT               [GA]^GATC[CT]
             1651  GGATCT              
EcoRI         259  GAATTC               G^AATTC
              631  GAATTC              
HapII         658  CCGG                 C^CGG
             1831  CCGG                
HincII        337  GTCAAC               GT[CT]^[GA]AC
             2515  GTCAAC              
HindII        337  GTCAAC               GT[CT]^[GA]AC
             2515  GTCAAC              
HpaII         658  CCGG                 C^CGG
             1831  CCGG                
MflI          234  GGATCT               [GA]^GATC[CT]
             1651  GGATCT              
MslI         2026  CACAAGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2535  CATCCACATG          
MspI          658  CCGG                 C^CGG
             1831  CCGG                
MvnI         2406  CGCG                 CG^CG
             2585  CGCG                
NspI         2069  GCATGT               [GA]CATG^[CT]
             2540  ACATGC              
PflFI         305  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              331  GACATTGTC           
PpuMI         170  AGGACCT              [GA]G^G[AT]CC[CT]
             1390  AGGTCCT             
SapI          754  GAAGAGC              GCTCTTC(1/4)
             1945  GAAGAGC             
Tth111I       305  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              331  GACATTGTC           
XhoII         234  GGATCT               [GA]^GATC[CT]
             1651  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApaI        ApoI        Asp700I     AspHI       
AvaII       BalI        BanII       BbsI        BbvI        
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstXI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EarI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HhaI        HinP1I      
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MseI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XmnI        
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