Restriction map for pF1KB8406
##################################
# NO CUT ENZYMES (  187 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaJI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DraII       DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT14I     
FauI        FbaI        Fnu4HI      FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        ItaI        
KasI        KpnI        KspI        MaeI        MaeII       
MamI        MluI        MluNI       MroI        MscI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NlaIV       
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacII       SalI        
SapI        Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StyI        SwaI        TfiI        
TseI        Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AciI          695  GCGG                 CCGC(-3/-1)
AfaI          716  GTAC                 GT^AC
AlwNI         468  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         294  GAGCTC               G[AT]GC[AT]^C
BanII         294  GAGCTC               G[GA]GC[CT]^C
BglII          48  AGATCT               A^GATCT
BsaI          152  GAGACC               GGTCTC(1/5)
BsaWI         412  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       294  GAGCTC               G[AT]GC[AT]^C
BsmAI         152  GAGAC                GTCTC(1/5)
BsrI          580  ACTGG                ACTGG(1/-1)
BstYI          48  AGATCT               [GA]^GATC[CT]
DraI          427  TTTAAA               TTT^AAA
DraIII        241  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          436  GAAGAG               CTCTTC(1/4)
HapII         413  CCGG                 C^CGG
HpaII         413  CCGG                 C^CGG
HphI          331  GGTGA                GGTGA(8/7)
Ksp632I       436  GAAGAG               CTCTTC(1/4)
MfeI          288  CAATTG               C^AATTG
MflI           48  AGATCT               [GA]^GATC[CT]
MspA1I         77  CAGCTG               C[AC]G^C[GT]G
MspI          413  CCGG                 C^CGG
MunI          288  CAATTG               C^AATTG
NdeI          313  CATATG               CA^TATG
PflMI         240  CCACACAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII          77  CAGCTG               CAG^CTG
RsaI          716  GTAC                 GT^AC
SacI          294  GAGCTC               GAGCT^C
Tsp45I         98  GTGAC                ^GT[GC]AC
Van91I        240  CCACACAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII          48  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AluI           78  AGCT                 AG^CT
              295  AGCT                
BmyI           12  GTGCCC               G[AGT]GC[ACT]^C
              294  GAGCTC              
BpmI          465  CTCCAG               CTGGAG(16/14)
              581  CTGGAG              
Bsp1286I       12  GTGCCC               G[AGT]GC[ACT]^C
              294  GAGCTC              
Cac8I         392  GCATGC               GC[ACGT]^[ACGT]GC
              476  GCATGC              
DdeI           38  CTGAG                C^T[ACGT]AG
               80  CTGAG               
DpnII           3  GATC                 ^GATC
               49  GATC                
MaeIII         98  GTGAC                ^GT[ACGT]AC
              266  GTTAC               
MboI            3  GATC                 ^GATC
               49  GATC                
MboII         436  GAAGA                GAAGA(8/7)
              303  TCTTC               
MseI          221  TTAA                 T^TAA
              428  TTAA                
MslI           91  CATAAATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              325  CATACAGGTG          
NdeII           3  GATC                 ^GATC
               49  GATC                
NspI          392  GCATGC               [GA]CATG^[CT]
              476  GCATGC              
Sau3AI          3  GATC                 ^GATC
               49  GATC                
SfcI          348  CTGTAG               C^T[GA][CT]AG
              594  CTATAG              
SphI          392  GCATGC               GCATG^C
              476  GCATGC              
TaqI          550  TCGA                 T^CGA
              634  TCGA                
Tru9I         221  TTAA                 T^TAA
              428  TTAA                
TspRI         245  CAGTG                CA[GC]TG[ACGT][ACGT]^
              579  CACTG               
TthHB8I       550  TCGA                 T^CGA
              634  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AcsI        ApoI        Asp700I     BsiYI       BslI        
BstF5I      EcoT22I     FokI        HaeIII      HinfI       
MnlI        NlaIII      NsiI        PleI        SfaNI       
StuI        Tsp509I     XmnI        
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