Restriction map for pF1KB8406
 (Linear) (Six-base) MAPSORT of: KIBB8406.seq  Check: 4664  from: 1  to: 783



KIBB8406.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     438     606     690     783 
   Size:        438     168      84      93

  Fragments arranged by size: 

                 438     168      93      84
 
AlwNI CAG_nnn'CTG

Cuts at:      0     473     783 
   Size:        473     310
 
ApoI r'AATT_y

Cuts at:      0      28      68     308     783 
   Size:         28      40     240     475

  Fragments arranged by size: 

                 475     240      40      28
 
BanII G_rGCy'C

Cuts at:      0     298     783 
   Size:        298     485
 
BfrBI ATG'CAT

Cuts at:      0      60     396     480     783 
   Size:         60     336      84     303

  Fragments arranged by size: 

                 336     303      84      60
 
BglII A'GATC_T

Cuts at:      0      48     783 
   Size:         48     735
 
Bme1580I G_kGCm'C

Cuts at:      0      16     783 
   Size:         16     767
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     450     602     783 
   Size:        450     152     181

  Fragments arranged by size: 

                 450     181     152
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     146     783 
   Size:        146     637
 
BsaWI w'CCGG_w

Cuts at:      0     412     783 
   Size:        412     371
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     155     185     783 
   Size:        155      30     598

  Fragments arranged by size: 

                 598     155      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     298     783 
   Size:        298     485
 
Bsp1286I G_dGCh'C

Cuts at:      0      16     298     783 
   Size:         16     282     485

  Fragments arranged by size: 

                 485     282      16
 
BstYI r'GATC_y

Cuts at:      0      48     783 
   Size:         48     735
 
DraI TTT'AAA

Cuts at:      0     429     783 
   Size:        429     354
 
DraIII CAC_nnn'GTG

Cuts at:      0     246     783 
   Size:        246     537
 
EarI CTCTTCn'nnn_

Cuts at:      0     431     783 
   Size:        431     352
 
EcoICRI GAG'CTC

Cuts at:      0     296     783 
   Size:        296     487
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     438     450     602     606     690     783 
   Size:        438      12     152       4      84      93

  Fragments arranged by size: 

                 438     152      93      84      12       4
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     135     783 
   Size:        135     648
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      33      65     155     187     285     317     747     779 
   Size:         33      32      90      32      98      32     430      32

Cuts at:    779     783 
   Size:          4

  Fragments arranged by size: 

                 430      98      90      33      32      32      32      32
                   4
 
MfeI C'AATT_G

Cuts at:      0     288     783 
   Size:        288     495
 
MslI CAynn'nnrTG

Cuts at:      0      95     329     783 
   Size:         95     234     454

  Fragments arranged by size: 

                 454     234      95
 
MspA1I CmG'CkG

Cuts at:      0      79     783 
   Size:         79     704
 
NdeI CA'TA_TG

Cuts at:      0     314     783 
   Size:        314     469
 
NsiI A_TGCA'T

Cuts at:      0      62     398     482     783 
   Size:         62     336      84     301

  Fragments arranged by size: 

                 336     301      84      62
 
NspI r_CATG'y

Cuts at:      0     396     480     783 
   Size:        396      84     303

  Fragments arranged by size: 

                 396     303      84
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     246     783 
   Size:        246     537
 
PvuII CAG'CTG

Cuts at:      0      79     783 
   Size:         79     704
 
SacI G_AGCT'C

Cuts at:      0     298     783 
   Size:        298     485
 
SfcI C'TryA_G

Cuts at:      0     348     594     783 
   Size:        348     246     189

  Fragments arranged by size: 

                 348     246     189
 
SphI G_CATG'C

Cuts at:      0     396     480     783 
   Size:        396      84     303

  Fragments arranged by size: 

                 396     303      84
 
StuI AGG'CCT

Cuts at:      0     451     619     703     783 
   Size:        451     168      84      80

  Fragments arranged by size: 

                 451     168      84      80
 
XmnI GAAnn'nnTTC

Cuts at:      0     451     535     619     676     783 
   Size:        451      84      84      57     107

  Fragments arranged by size: 

                 451     107      84      84      57
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI    BanII    BfrBI    BglII Bme1580I     BpmI
     BsaI    BsaWI    BsaXI  BsiHKAI Bsp1286I    BstYI     DraI   DraIII
     EarI  EcoICRI  Eco57MI     FalI    Hin4I     MfeI     MslI   MspA1I
     NdeI     NsiI     NspI    PflMI    PvuII     SacI     SfcI     SphI
     StuI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DrdI     EaeI     EagI     EciI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI
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