Restriction map for pF1KB8402
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AgeI        
AhdI        AocI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvrII       BalI        
BbeI        BbrPI       BbsI        BciVI       BclI        
BfrI        BglII       BlnI        BpuAI       BsaBI       
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspHI       BspLU11I    BsrBI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoO65I     EcoRV       
FbaI        HincII      HindII      HpaI        KasI        
KpnI        Ksp632I     MamI        MfeI        MluI        
MluNI       MscI        MslI        MunI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SalI        
SapI        ScaI        SexAI       SfoI        SfuI        
SmaI        SmlI        SnaBI       SpeI        SspBI       
SspI        SwaI        Tth111I     XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AatII         532  GACGTC               GACGT^C
AflIII        640  ACACGT               A^C[GA][CT]GT
AviII        1197  TGCGCA               TGC^GCA
BamHI         373  GGATCC               G^GATCC
BbvCI        2157  GCTGAGG              CCTCAGC(-5/-2)
BcnI          957  CCGGG                CC^[GC]GG
BsaAI         641  CACGTA               [CT]AC^GT[GA]
BspMI        1236  GCAGGT               ACCTGC(4/8)
BstAPI        893  GCACCCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         503  CCAGAAATCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoRI         315  GAATTC               G^AATTC
EcoT22I       259  ATGCAT               ATGCA^T
FseI          965  GGCCGGCC             GGCCGG^CC
FspI         1197  TGCGCA               TGC^GCA
HindIII      2236  AAGCTT               A^AGCTT
KspI          987  CCGCGG               CCGC^GG
MboII         516  TCTTC                GAAGA(8/7)
MseI         2297  TTAA                 T^TAA
NaeI          966  GCCGGC               GCC^GGC
NciI          957  CCGGG                CC^[GC]GG
NgoMIV        966  GCCGGC               G^CCGGC
NsiI          259  ATGCAT               ATGCA^T
PflMI        1450  CCAGAGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         498  AGGTCCC              [GA]G^G[AT]CC[CT]
PshAI         129  GACTCTCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII         987  CCGCGG               CCGC^GG
SfiI         2180  GGCCCGAGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI         940  CGCCGGTG             C[GA]^CCGG[CT]G
SphI         1481  GCATGC               GCATG^C
Tru9I        2297  TTAA                 T^TAA
Van91I       1450  CCAGAGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          360  CCAGTGTTCCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccIII        803  TCCGGA               T^CCGGA
             1869  TCCGGA              
AcsI          315  GAATTC               [GA]^AATT[CT]
              658  AAATTC              
AcyI          433  GACGCC               G[GA]^CG[CT]C
              532  GACGTC              
AfaI          706  GTAC                 GT^AC
             2257  GTAC                
ApaI          795  GGGCCC               GGGCC^C
             1099  GGGCCC              
ApoI          315  GAATTC               [GA]^AATT[CT]
              658  AAATTC              
Asp700I      1350  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2214  GAAGGGCTTC          
BcgI          404  GCATCACCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1292  GCAAGGGCTTCG        
BsaHI         433  GACGCC               G[GA]^CG[CT]C
              532  GACGTC              
BsaI         1266  GAGACC               GGTCTC(1/5)
             1770  GAGACC              
BseAI         803  TCCGGA               T^CCGGA
             1869  TCCGGA              
BseRI         246  CTCCTC               GAGGAG(10/8)
              443  CTCCTC              
BsmBI         135  CGTCTC               CGTCTC(1/5)
              676  GAGACG              
BspEI         803  TCCGGA               T^CCGGA
             1869  TCCGGA              
BsrDI        1201  CATTGC               GCAATG(2/0)
             2323  GCAATG              
BssSI         835  CACGAG               CACGAG(-5/-1)
             1695  CTCGTG              
DsaI          688  CCGTGG               C^C[GA][CT]GG
              987  CCGCGG              
EcoT14I       399  CCAAGG               C^C[AT][AT]GG
              562  CCTTGG              
HgaI          433  GACGC                GACGC(5/10)
              678  GACGC               
Hin1I         433  GACGCC               G[GA]^CG[CT]C
              532  GACGTC              
MaeII         533  ACGT                 A^CGT
              642  ACGT                
MaeIII       2042  GTGAC                ^GT[ACGT]AC
             2126  GTGAC               
MroI          803  TCCGGA               T^CCGGA
             1869  TCCGGA              
PleI          129  GACTC                GAGTC(4/5)
             1334  GAGTC               
RsaI          706  GTAC                 GT^AC
             2257  GTAC                
SbfI         1160  CCTGCAGG             CCTGCA^GG
             1277  CCTGCAGG            
Sse8387I     1160  CCTGCAGG             CCTGCA^GG
             1277  CCTGCAGG            
StuI          391  AGGCCT               AGG^CCT
             2104  AGGCCT              
StyI          399  CCAAGG               C^C[AT][AT]GG
              562  CCTTGG              
TaqI         1332  TCGA                 T^CGA
             1991  TCGA                
Tsp45I       2042  GTGAC                ^GT[GC]AC
             2126  GTGAC               
TthHB8I      1332  TCGA                 T^CGA
             1991  TCGA                
XbaI           75  TCTAGA               T^CTAGA
              141  TCTAGA              
XmnI         1350  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2214  GAAGGGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AccII       AciI        AluI        Alw44I      AlwI        
AlwNI       Aor51HI     ApaLI       AspHI       AvaI        
AvaII       BanI        BanII       BbvI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BsaJI       BsaWI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
Eco47III    EcoNI       EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MboI        MflI        MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PstI        
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TfiI        TseI        Tsp509I     TspRI       
XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com