Restriction map for pF1KB7636
 (Linear) (Six-base) MAPSORT of: KIBB7636.seq  Check: 5380  from: 1  to: 1131



KIBB7636.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AccI GT'mk_AC

Cuts at:      0     752    1131 
   Size:        752     379
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     335    1131 
   Size:        335     796
 
AflII C'TTAA_G

Cuts at:      0     862    1131 
   Size:        862     269
 
AflIII A'CryG_T

Cuts at:      0     638    1131 
   Size:        638     493
 
AlwNI CAG_nnn'CTG

Cuts at:      0      39     313    1131 
   Size:         39     274     818

  Fragments arranged by size: 

                 818     274      39
 
ApoI r'AATT_y

Cuts at:      0     502     604    1131 
   Size:        502     102     527

  Fragments arranged by size: 

                 527     502     102
 
AvaI C'yCGr_G

Cuts at:      0      64    1131 
   Size:         64    1067
 
AvrII C'CTAG_G

Cuts at:      0     654     966    1131 
   Size:        654     312     165

  Fragments arranged by size: 

                 654     312     165
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     660     693    1131 
   Size:        660      33     438

  Fragments arranged by size: 

                 660     438      33
 
BamHI G'GATC_C

Cuts at:      0     813    1131 
   Size:        813     318
 
BanII G_rGCy'C

Cuts at:      0    1076    1131 
   Size:       1076      55
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     563    1131 
   Size:        563     568
 
BclI T'GATC_A

Cuts at:      0     264    1131 
   Size:        264     867
 
BglI GCCn_nnn'nGGC

Cuts at:      0     254     931    1131 
   Size:        254     677     200

  Fragments arranged by size: 

                 677     254     200
 
BglII A'GATC_T

Cuts at:      0     725    1131 
   Size:        725     406
 
Bme1580I G_kGCm'C

Cuts at:      0     689    1131 
   Size:        689     442
 
BmgBI CAC'GTC

Cuts at:      0     639    1131 
   Size:        639     492
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     434     824    1131 
   Size:        220     214     390     307

  Fragments arranged by size: 

                 390     307     220     214
 
BsaAI yAC'GTr

Cuts at:      0     522    1131 
   Size:        522     609
 
BsaBI GATnn'nnATC

Cuts at:      0     221     263     266     530    1131 
   Size:        221      42       3     264     601

  Fragments arranged by size: 

                 601     264     221      42       3
 
BsaWI w'CCGG_w

Cuts at:      0     270    1131 
   Size:        270     861
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     536     566     592     622    1131 
   Size:        536      30      26      30     509

  Fragments arranged by size: 

                 536     509      30      30      26
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     208     920     923    1131 
   Size:         19     189     712       3     208

  Fragments arranged by size: 

                 712     208     189      19       3
 
BseYI C'CCAG_C

Cuts at:      0     306     789     808    1131 
   Size:        306     483      19     323

  Fragments arranged by size: 

                 483     323     306      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     583    1131 
   Size:        520      63     548

  Fragments arranged by size: 

                 548     520      63
 
BsiHKAI G_wGCw'C

Cuts at:      0     826    1131 
   Size:        826     305
 
BsmI GAATG_Cn'

Cuts at:      0     123     562    1131 
   Size:        123     439     569

  Fragments arranged by size: 

                 569     439     123
 
Bsp1286I G_dGCh'C

Cuts at:      0     689     826    1076    1131 
   Size:        689     137     250      55

  Fragments arranged by size: 

                 689     250     137      55
 
BspEI T'CCGG_A

Cuts at:      0     270    1131 
   Size:        270     861
 
BspHI T'CATG_A

Cuts at:      0     584     999    1131 
   Size:        584     415     132

  Fragments arranged by size: 

                 584     415     132
 
BsrDI GCAATG_nn'

Cuts at:      0    1074    1131 
   Size:       1074      57
 
BsrFI r'CCGG_y

Cuts at:      0      18    1055    1131 
   Size:         18    1037      76

  Fragments arranged by size: 

                1037      76      18
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     547     777    1131 
   Size:        547     230     354

  Fragments arranged by size: 

                 547     354     230
 
BstYI r'GATC_y

Cuts at:      0      98     531     725     813     989    1131 
   Size:         98     433     194      88     176     142

  Fragments arranged by size: 

                 433     194     176     142      98      88
 
BstZ17I GTA'TAC

Cuts at:      0     753    1131 
   Size:        753     378
 
BtgI C'CryG_G

Cuts at:      0     188     540    1131 
   Size:        188     352     591

  Fragments arranged by size: 

                 591     352     188
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     272    1131 
   Size:        272     859
 
BtsI GCAGTG_nn'

Cuts at:      0     145     782    1131 
   Size:        145     637     349

  Fragments arranged by size: 

                 637     349     145
 
EaeI y'GGCC_r

Cuts at:      0      92     200    1057    1131 
   Size:         92     108     857      74

  Fragments arranged by size: 

                 857     108      92      74
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      37    1131 
   Size:         37    1094
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     335     434     824    1131 
   Size:        220     115      99     390     307

  Fragments arranged by size: 

                 390     307     220     115      99
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     658    1131 
   Size:        658     473
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     430     462     981    1013    1131 
   Size:        430      32     519      32     118

  Fragments arranged by size: 

                 519     430     118      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1110    1131 
   Size:       1110      21
 
MscI TGG'CCA

Cuts at:      0      94     202    1131 
   Size:         94     108     929

  Fragments arranged by size: 

                 929     108      94
 
MslI CAynn'nnrTG

Cuts at:      0     215     545     589    1131 
   Size:        215     330      44     542

  Fragments arranged by size: 

                 542     330     215      44
 
MspA1I CmG'CkG

Cuts at:      0     364     789    1131 
   Size:        364     425     342

  Fragments arranged by size: 

                 425     364     342
 
NaeI GCC'GGC

Cuts at:      0    1057    1131 
   Size:       1057      74
 
NcoI C'CATG_G

Cuts at:      0     188     540    1131 
   Size:        188     352     591

  Fragments arranged by size: 

                 591     352     188
 
NdeI CA'TA_TG

Cuts at:      0     141    1017    1131 
   Size:        141     876     114

  Fragments arranged by size: 

                 876     141     114
 
NgoMIV G'CCGG_C

Cuts at:      0    1055    1131 
   Size:       1055      76
 
NspI r_CATG'y

Cuts at:      0    1126    1131 
   Size:       1126       5
 
PshAI GACnn'nnGTC

Cuts at:      0     958    1131 
   Size:        958     173
 
PstI C_TGCA'G

Cuts at:      0     230    1131 
   Size:        230     901
 
PvuII CAG'CTG

Cuts at:      0     364     789    1131 
   Size:        364     425     342

  Fragments arranged by size: 

                 425     364     342
 
SfcI C'TryA_G

Cuts at:      0      57     226     341     672    1131 
   Size:         57     169     115     331     459

  Fragments arranged by size: 

                 459     331     169     115      57
 
SmlI C'TyrA_G

Cuts at:      0     862    1131 
   Size:        862     269
 
SspI AAT'ATT

Cuts at:      0     485     718    1131 
   Size:        485     233     413

  Fragments arranged by size: 

                 485     413     233
 
StyI C'CwwG_G

Cuts at:      0     188     540     654     966    1131 
   Size:        188     352     114     312     165

  Fragments arranged by size: 

                 352     312     188     165     114
 
TatI w'GTAC_w

Cuts at:      0     738     866    1131 
   Size:        738     128     265

  Fragments arranged by size: 

                 738     265     128
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     592     624     805     837    1131 
   Size:        592      32     181      32     294

  Fragments arranged by size: 

                 592     294     181      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     591    1131 
   Size:        591     540
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     195    1131 
   Size:        195     936
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII    AlwNI     ApoI     AvaI    AvrII
     BaeI    BamHI    BanII     BbsI     BclI     BglI    BglII Bme1580I
    BmgBI     BpmI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BsrDI    BsrFI
    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI     EaeI     EciI
  Eco57MI    EcoNI    Hin4I     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI     NdeI   NgoMIV     NspI    PshAI     PstI    PvuII     SfcI
     SmlI     SspI     StyI     TatI     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     BanI
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BlpI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaHI    BsiEI    BsiWI    BsmBI
    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NarI     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII
     XbaI     XhoI     XmaI     XmnI     ZraI
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