Restriction map for pF1KB7630
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BbsI        BcgI        BclI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmAI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstYI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraII       DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoRI       
EcoRV       FbaI        FseI        FspI        Hin1I       
HindIII     HpaI        KasI        KspI        MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspA1I      MunI        NaeI        NarI        
NcoI        NgoMIV      NotI        NruI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SwaI        
TfiI        Tth111I     XbaI        XcmI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I        891  GGTACC               G^GTACC
AccII         691  CGCG                 CG^CG
AflII         319  CTTAAG               C^TTAAG
AflIII        757  ACACGT               A^C[GA][CT]GT
AlwNI        1053  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI           18  GGGCCC               GGGCC^C
Asp718I       891  GGTACC               G^GTACC
AvaI          637  CTCGAG               C^[CT]CG[GA]G
BanI          891  GGTACC               G^G[CT][GA]CC
BbvCI         681  CCTCAGC              CCTCAGC(-5/-2)
BciVI          33  GTATCC               GTATCC(6/5)
BcnI          968  CCCGG                CC^[GC]GG
BfaI          866  CTAG                 C^TAG
BfrI          319  CTTAAG               C^TTAAG
BseRI         139  GAGGAG               GAGGAG(10/8)
BsgI         1037  CTGCAC               GTGCAG(16/14)
BsoBI         637  CTCGAG               C^[CT]CG[GA]G
BspMI         706  ACCTGC               ACCTGC(4/8)
BsrFI         431  GCCGGT               [GA]^CCGG[CT]
BstAPI        739  GCACCCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        384  GGTGACC              G^GT[ACGT]ACC
BstPI         384  GGTGACC              G^GT[ACGT]ACC
BstUI         691  CGCG                 CG^CG
BstXI         920  CCACCCTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        431  GCCGGT               [GA]^CCGG[CT]
DraIII        582  CACTCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          249  TGGCCG               [CT]^GGCC[GA]
EarI          855  GAAGAG               CTCTTC(1/4)
EcoO65I       384  GGTGACC              G^GT[ACGT]ACC
EcoT14I       329  CCAAGG               C^C[AT][AT]GG
HaeII        1045  GGCGCT               [GA]GCGC^[CT]
HgaI          991  GACGC                GACGC(5/10)
HincII        201  GTTGAC               GT[CT]^[GA]AC
HindII        201  GTTGAC               GT[CT]^[GA]AC
HinfI         623  GACTC                G^A[ACGT]TC
KpnI          891  GGTACC               GGTAC^C
Ksp632I       855  GAAGAG               CTCTTC(1/4)
MaeI          866  CTAG                 C^TAG
MvnI          691  CGCG                 CG^CG
NciI          968  CCCGG                CC^[GC]GG
NdeI           77  CATATG               CA^TATG
NheI          865  GCTAGC               G^CTAGC
NspI          537  GCATGC               [GA]CATG^[CT]
PaeR7I        637  CTCGAG               C^TCGAG
PleI          623  GACTC                GAGTC(4/5)
SapI          855  GAAGAGC              GCTCTTC(1/4)
SphI          537  GCATGC               GCATG^C
SspI          102  AATATT               AAT^ATT
StuI         1115  AGGCCT               AGG^CCT
StyI          329  CCAAGG               C^C[AT][AT]GG
Tsp509I       601  AATT                 ^AATT
TspRI        1040  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoI          637  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwI          261  GATCC                GGATC(4/5)
              561  GGATC               
AvaII         229  GGACC                G^G[AT]CC
              254  GGACC               
BsmFI         944  GGGAC                GGGAC(10/14)
              357  GTCCC               
BsmI          166  GCATTC               GAATGC(1/-1)
              337  GCATTC              
CfoI          729  GCGC                 GCG^C
             1046  GCGC                
DdeI          176  CTGAG                C^T[ACGT]AG
              682  CTCAG               
DpnII         261  GATC                 ^GATC
              562  GATC                
EcoT22I       164  ATGCAT               ATGCA^T
              535  ATGCAT              
HhaI          729  GCGC                 GCG^C
             1046  GCGC                
HinP1I        729  GCGC                 G^CGC
             1046  GCGC                
MboI          261  GATC                 ^GATC
              562  GATC                
MslI          411  CACCCAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              921  CACCCTGATG          
NdeII         261  GATC                 ^GATC
              562  GATC                
NsiI          164  ATGCAT               ATGCA^T
              535  ATGCAT              
PflMI          55  CCAGGGATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              552  CCAGATGTTGG         
Sau3AI        261  GATC                 ^GATC
              562  GATC                
SfcI           96  CTACAG               C^T[GA][CT]AG
              675  CTACAG              
TaqI          362  TCGA                 T^CGA
              638  TCGA                
TthHB8I       362  TCGA                 T^CGA
              638  TCGA                
Van91I         55  CCAGGGATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              552  CCAGATGTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       BanII       
BbvI        BmyI        BpmI        BsaJI       BsiHKAI     
BsiYI       BslI        Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboII       MnlI        MseI        MspI        MvaI        
MwoI        NlaIII      NlaIV       RsaI        SacI        
Sau96I      ScrFI       SfaNI       SmlI        Tru9I       
TseI        Tsp45I      
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