Restriction map for pF1KB7630
 (Linear) (Six-base) MAPSORT of: KIBB7630.seq  Check: 1  from: 1  to: 1125



KIBB7630.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

Acc65I G'GTAC_C

Cuts at:      0     891    1125 
   Size:        891     234
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     471    1125 
   Size:        109     362     654

  Fragments arranged by size: 

                 654     362     109
 
AflII C'TTAA_G

Cuts at:      0     319    1125 
   Size:        319     806
 
AflIII A'CryG_T

Cuts at:      0     757    1125 
   Size:        757     368
 
AleI CACnn'nnGTG

Cuts at:      0     415    1125 
   Size:        415     710
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1058    1125 
   Size:       1058      67
 
ApaI G_GGCC'C

Cuts at:      0      22    1125 
   Size:         22    1103
 
AvaI C'yCGr_G

Cuts at:      0     637    1125 
   Size:        637     488
 
BanI G'GyrC_C

Cuts at:      0     891    1125 
   Size:        891     234
 
BanII G_rGCy'C

Cuts at:      0      22     512     644     656     755     817     862     920 
   Size:         22     490     132      12      99      62      45      58

Cuts at:    920    1125 
   Size:        205

  Fragments arranged by size: 

                 490     205     132      99      62      58      45      22
                  12
 
BbvCI CC'TCA_GC

Cuts at:      0     682    1125 
   Size:        682     443
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      44    1125 
   Size:         44    1081
 
BfrBI ATG'CAT

Cuts at:      0     166     537    1125 
   Size:        166     371     588

  Fragments arranged by size: 

                 588     371     166
 
Bme1580I G_kGCm'C

Cuts at:      0      22    1125 
   Size:         22    1103
 
BmgBI CAC'GTC

Cuts at:      0     479     760    1109    1125 
   Size:        479     281     349      16

  Fragments arranged by size: 

                 479     349     281      16
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     978    1051    1125 
   Size:        978      73      74

  Fragments arranged by size: 

                 978      74      73
 
BmtI G_CTAG'C

Cuts at:      0     869    1125 
   Size:        869     256
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     241     463     867     994    1125 
   Size:         70     171     222     404     127     131

  Fragments arranged by size: 

                 404     222     171     131     127      70
 
Bpu10I CC'TnA_GC

Cuts at:      0     682    1125 
   Size:        682     443
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     685    1125 
   Size:        685     440
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     418     436     448     466    1125 
   Size:        418      18      12      18     659

  Fragments arranged by size: 

                 659     418      18      18      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     154    1125 
   Size:        154     971
 
BseYI C'CCAG_C

Cuts at:      0     389     769     861    1125 
   Size:        389     380      92     264

  Fragments arranged by size: 

                 389     380     264      92
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1022    1125 
   Size:       1022     103
 
BsiHKAI G_wGCw'C

Cuts at:      0     512     656     817    1125 
   Size:        512     144     161     308

  Fragments arranged by size: 

                 512     308     161     144
 
BsmI GAATG_Cn'

Cuts at:      0     166     337    1125 
   Size:        166     171     788

  Fragments arranged by size: 

                 788     171     166
 
Bsp1286I G_dGCh'C

Cuts at:      0      22     512     644     656     755     817     862     920 
   Size:         22     490     132      12      99      62      45      58

Cuts at:    920    1125 
   Size:        205

  Fragments arranged by size: 

                 490     205     132      99      62      58      45      22
                  12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     715    1125 
   Size:        715     410
 
BsrFI r'CCGG_y

Cuts at:      0     431    1125 
   Size:        431     694
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     745    1125 
   Size:        745     380
 
BstEII G'GTnAC_C

Cuts at:      0     384    1125 
   Size:        384     741
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     927    1125 
   Size:        927     198
 
DraIII CAC_nnn'GTG

Cuts at:      0     587    1125 
   Size:        587     538
 
EaeI y'GGCC_r

Cuts at:      0     249    1125 
   Size:        249     876
 
EarI CTCTTCn'nnn_

Cuts at:      0     850    1125 
   Size:        850     275
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      26     795    1125 
   Size:         26     769     330

  Fragments arranged by size: 

                 769     330      26
 
EcoICRI GAG'CTC

Cuts at:      0     510     654     815    1125 
   Size:        510     144     161     310

  Fragments arranged by size: 

                 510     310     161     144
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     109     241     463     471     867     994    1125 
   Size:         70      39     132     222       8     396     127     131

  Fragments arranged by size: 

                 396     222     132     131     127      70      39       8
            
 
HaeII r_GCGC'y

Cuts at:      0    1049    1125 
   Size:       1049      76
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     497     529     546     553     578     585     643     675 
   Size:        497      32      17       7      25       7      58      32

Cuts at:    675    1125 
   Size:        450

  Fragments arranged by size: 

                 497     450      58      32      32      25      17       7
                   7
 
HincII GTy'rAC

Cuts at:      0     203    1125 
   Size:        203     922
 
KpnI G_GTAC'C

Cuts at:      0     895    1125 
   Size:        895     230
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     539     645    1125 
   Size:        539     106     480

  Fragments arranged by size: 

                 539     480     106
 
MslI CAynn'nnrTG

Cuts at:      0     415     925    1125 
   Size:        415     510     200

  Fragments arranged by size: 

                 510     415     200
 
NdeI CA'TA_TG

Cuts at:      0      78    1125 
   Size:         78    1047
 
NheI G'CTAG_C

Cuts at:      0     865    1125 
   Size:        865     260
 
NsiI A_TGCA'T

Cuts at:      0     168     539    1125 
   Size:        168     371     586

  Fragments arranged by size: 

                 586     371     168
 
NspI r_CATG'y

Cuts at:      0     541    1125 
   Size:        541     584
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      61     558    1125 
   Size:         61     497     567

  Fragments arranged by size: 

                 567     497      61
 
PspOMI G'GGCC_C

Cuts at:      0      18    1125 
   Size:         18    1107
 
PspXI vC'TCGA_Gb

Cuts at:      0     637    1125 
   Size:        637     488
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     430     462    1125 
   Size:        430      32     663

  Fragments arranged by size: 

                 663     430      32
 
SacI G_AGCT'C

Cuts at:      0     512     656     817    1125 
   Size:        512     144     161     308

  Fragments arranged by size: 

                 512     308     161     144
 
SapI GCTCTTCn'nnn_

Cuts at:      0     850    1125 
   Size:        850     275
 
SfcI C'TryA_G

Cuts at:      0      96     675    1125 
   Size:         96     579     450

  Fragments arranged by size: 

                 579     450      96
 
SmlI C'TyrA_G

Cuts at:      0     319     637     700    1125 
   Size:        319     318      63     425

  Fragments arranged by size: 

                 425     319     318      63
 
SphI G_CATG'C

Cuts at:      0     541    1125 
   Size:        541     584
 
SspI AAT'ATT

Cuts at:      0     104    1125 
   Size:        104    1021
 
StuI AGG'CCT

Cuts at:      0    1117    1125 
   Size:       1117       8
 
StyI C'CwwG_G

Cuts at:      0     329    1125 
   Size:        329     796
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     427     997    1125 
   Size:        427     570     128

  Fragments arranged by size: 

                 570     427     128
 
TatI w'GTAC_w

Cuts at:      0     308    1125 
   Size:        308     817
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     257     289    1125 
   Size:        257      32     836

  Fragments arranged by size: 

                 836     257      32
 
XhoI C'TCGA_G

Cuts at:      0     637    1125 
   Size:        637     488
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII   AflIII     AleI    AlwNI     ApaI     AvaI
     BanI    BanII    BbvCI    BciVI    BfrBI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrFI   BstAPI   BstEII    BstXI
   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI    HaeII    Hin4I
   HincII     KpnI     MmeI     MslI     NdeI     NheI     NsiI     NspI
    PflMI   PspOMI    PspXI     PsrI     SacI     SapI     SfcI     SmlI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI     AgeI     AhdI     AlfI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BbsI     BcgI     BclI     BglI    BglII     BlpI
     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
    BstBI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EagI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI
     NruI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI     PstI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI  Tth111I     XbaI
     XcmI     XmaI     XmnI     ZraI
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