Restriction map for pF1KB7896
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AgeI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspI        AvaI        AviII       
AvrII       BalI        BanI        BbeI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BsaBI       BsiEI       BsiWI       
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrGI       BssHII      Bst1107I    BstBI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DrdI        DsaI        EaeI        EagI        EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoRI       EcoRV       FauI        FbaI        FseI        
FspI        HaeII       HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MamI        MfeI        MluI        MluNI       MscI        
MunI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
Van91I      XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccII          16  CGCG                 CG^CG
AccIII        752  TCCGGA               T^CCGGA
AcyI          459  GACGCC               G[GA]^CG[CT]C
AflIII        574  ACACGT               A^C[GA][CT]GT
AhdI          269  GACCCAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         269  GACCCAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII        1683  GAGCTC               G[GA]GC[CT]^C
BbrPI         575  CACGTG               CAC^GTG
BbsI          456  GAAGAC               GAAGAC(2/6)
BcnI           30  CCCGG                CC^[GC]GG
BpuAI         456  GAAGAC               GAAGAC(2/6)
BsaAI         575  CACGTG               [CT]AC^GT[GA]
BsaHI         459  GACGCC               G[GA]^CG[CT]C
BseAI         752  TCCGGA               T^CCGGA
BsmBI          18  CGTCTC               CGTCTC(1/5)
BspEI         752  TCCGGA               T^CCGGA
BsrBI        1369  GAGCGG               CCGCTC(-3/-3)
BsrFI         435  GCCGGT               [GA]^CCGG[CT]
BssSI         946  CACGAG               CACGAG(-5/-1)
BstAPI       1487  GCAATGACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        167  GGTTACC              G^GT[ACGT]ACC
BstPI         167  GGTTACC              G^GT[ACGT]ACC
BstUI          16  CGCG                 CG^CG
BstXI        1152  CCATGAGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI           15  GCGC                 GCG^C
Cfr10I        435  GCCGGT               [GA]^CCGG[CT]
DraIII        816  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      269  GACCCAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       167  GGTTACC              G^GT[ACGT]ACC
EcoT22I      1901  ATGCAT               ATGCA^T
HhaI           15  GCGC                 GCG^C
Hin1I         459  GACGCC               G[GA]^CG[CT]C
HinP1I         15  GCGC                 G^CGC
MroI          752  TCCGGA               T^CCGGA
MseI          742  TTAA                 T^TAA
MspA1I         88  CAGCTG               C[AC]G^C[GT]G
MvnI           16  CGCG                 CG^CG
NciI           30  CCCGG                CC^[GC]GG
NdeI         1898  CATATG               CA^TATG
NsiI         1901  ATGCAT               ATGCA^T
NspI         1903  GCATGC               [GA]CATG^[CT]
PmaCI         575  CACGTG               CAC^GTG
PmlI          575  CACGTG               CAC^GTG
PstI          369  CTGCAG               CTGCA^G
PvuII          88  CAGCTG               CAG^CTG
SacI         1683  GAGCTC               GAGCT^C
SfcI          369  CTGCAG               C^T[GA][CT]AG
SphI         1903  GCATGC               GCATG^C
Tru9I         742  TTAA                 T^TAA
Tsp509I       843  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwNI        1027  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1908  CAGGGACTG           
BamHI        1625  GGATCC               G^GATCC
             1877  GGATCC              
BbvI           87  GCAGC                GCAGC(8/12)
              899  GCAGC               
BlpI          677  GCTCAGC              GC^T[ACGT]AGC
             1776  GCTCAGC             
BpmI          196  CTGGAG               CTGGAG(16/14)
              469  CTGGAG              
Bpu1102I      677  GCTCAGC              GC^T[ACGT]AGC
             1776  GCTCAGC             
BsaI          717  GAGACC               GGTCTC(1/5)
              150  GGTCTC              
BsgI          981  GTGCAG               GTGCAG(16/14)
             1401  GTGCAG              
BsrDI        1487  GCAATG               GCAATG(2/0)
             1739  GCAATG              
BstYI        1625  GGATCC               [GA]^GATC[CT]
             1877  GGATCC              
CelII         677  GCTCAGC              GC^T[ACGT]AGC
             1776  GCTCAGC             
EcoT14I       240  CCAAGG               C^C[AT][AT]GG
             1718  CCAAGG              
MboII         310  GAAGA                GAAGA(8/7)
              456  GAAGA               
MflI         1625  GGATCC               [GA]^GATC[CT]
             1877  GGATCC              
StyI          240  CCAAGG               C^C[AT][AT]GG
             1718  CCAAGG              
TaqI          689  TCGA                 T^CGA
             1697  TCGA                
TseI           87  GCAGC                G^C[AT]GC
              899  GCAGC               
TthHB8I       689  TCGA                 T^CGA
             1697  TCGA                
XbaI          229  TCTAGA               T^CTAGA
              417  TCTAGA              
XhoII        1625  GGATCC               [GA]^GATC[CT]
             1877  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        Asp700I     
AspHI       AvaII       BfaI        BmyI        BsaJI       
BsaWI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HinfI       HpaII       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MnlI        MslI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       StuI        TfiI        
Tsp45I      TspRI       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com