Restriction map for pF1KB7896
 (Linear) (Six-base) MAPSORT of: KIBB7896.seq  Check: 8008  from: 1  to: 1974



KIBB7896.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     112     123     329     903     921    1071    1239 
   Size:         51      61      11     206     574      18     150     168

Cuts at:   1239    1323    1575    1659    1743    1974 
   Size:         84     252      84      84     231

  Fragments arranged by size: 

                 574     252     231     206     168     150      84      84
                  84      61      51      18      11
 
AflIII A'CryG_T

Cuts at:      0     574    1974 
   Size:        574    1400
 
AhdI GACnn_n'nnGTC

Cuts at:      0     274    1974 
   Size:        274    1700
 
AleI CACnn'nnGTG

Cuts at:      0     451     878    1974 
   Size:        451     427    1096

  Fragments arranged by size: 

                1096     451     427
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1032    1913    1974 
   Size:       1032     881      61

  Fragments arranged by size: 

                1032     881      61
 
BamHI G'GATC_C

Cuts at:      0    1625    1877    1974 
   Size:       1625     252      97

  Fragments arranged by size: 

                1625     252      97
 
BanII G_rGCy'C

Cuts at:      0    1687    1974 
   Size:       1687     287
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     463    1974 
   Size:        463    1511
 
BfrBI ATG'CAT

Cuts at:      0    1903    1974 
   Size:       1903      71
 
BlpI GC'TnA_GC

Cuts at:      0     678    1777    1974 
   Size:        678    1099     197

  Fragments arranged by size: 

                1099     678     197
 
BmgBI CAC'GTC

Cuts at:      0      54    1974 
   Size:         54    1920
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     405     803    1055    1559    1811    1974 
   Size:        405     398     252     504     252     163

  Fragments arranged by size: 

                 504     405     398     252     252     163
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     490    1974 
   Size:        217     273    1484

  Fragments arranged by size: 

                1484     273     217
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     156     711    1974 
   Size:        156     555    1263

  Fragments arranged by size: 

                1263     555     156
 
BsaAI yAC'GTr

Cuts at:      0     577    1974 
   Size:        577    1397
 
BsaHI Gr'CG_yC

Cuts at:      0     460    1974 
   Size:        460    1514
 
BsaWI w'CCGG_w

Cuts at:      0     698     752    1871    1974 
   Size:        698      54    1119     103

  Fragments arranged by size: 

                1119     698     103      54
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1924    1954    1974 
   Size:       1924      30      20

  Fragments arranged by size: 

                1924      30      20
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      91    1341    1761    1929    1974 
   Size:         91    1250     420     168      45

  Fragments arranged by size: 

                1250     420     168      91      45
 
BseYI C'CCAG_C

Cuts at:      0    1025    1974 
   Size:       1025     949
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1002    1422    1974 
   Size:       1002     420     552

  Fragments arranged by size: 

                1002     552     420
 
BsiHKAI G_wGCw'C

Cuts at:      0    1267    1435    1687    1855    1974 
   Size:       1267     168     252     168     119

  Fragments arranged by size: 

                1267     252     168     168     119
 
BsmI GAATG_Cn'

Cuts at:      0     658     742     901    1489    1504    1573    1974 
   Size:        658      84     159     588      15      69     401

  Fragments arranged by size: 

                 658     588     401     159      84      69      15
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      24    1974 
   Size:         24    1950
 
Bsp1286I G_dGCh'C

Cuts at:      0    1267    1435    1687    1855    1974 
   Size:       1267     168     252     168     119

  Fragments arranged by size: 

                1267     252     168     168     119
 
BspEI T'CCGG_A

Cuts at:      0     752    1974 
   Size:        752    1222
 
BsrBI CCG'CTC

Cuts at:      0    1371    1974 
   Size:       1371     603
 
BsrDI GCAATG_nn'

Cuts at:      0    1494    1746    1974 
   Size:       1494     252     228

  Fragments arranged by size: 

                1494     252     228
 
BsrFI r'CCGG_y

Cuts at:      0     435    1974 
   Size:        435    1539
 
BssSI C'ACGA_G

Cuts at:      0     946    1974 
   Size:        946    1028
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1493    1974 
   Size:       1493     481
 
BstEII G'GTnAC_C

Cuts at:      0     167    1974 
   Size:        167    1807
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1159    1974 
   Size:       1159     815
 
BstYI r'GATC_y

Cuts at:      0    1625    1877    1974 
   Size:       1625     252      97

  Fragments arranged by size: 

                1625     252      97
 
BtsI GCAGTG_nn'

Cuts at:      0     437     481     990    1974 
   Size:        437      44     509     984

  Fragments arranged by size: 

                 984     509     437      44
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1308    1343    1560    1595    1728    1763    1974 
   Size:       1308      35     217      35     133      35     211

  Fragments arranged by size: 

                1308     217     211     133      35      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     821    1974 
   Size:        821    1153
 
EcoICRI GAG'CTC

Cuts at:      0    1685    1974 
   Size:       1685     289
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     112     123     217     329     490     903     921 
   Size:         51      61      11      94     112     161     413      18

Cuts at:    921    1071    1239    1323    1575    1659    1743    1974 
   Size:        150     168      84     252      84      84     231

  Fragments arranged by size: 

                 413     252     231     168     161     150     112      94
                  84      84      84      61      51      18      11
 
EcoO109I rG'GnC_Cy

Cuts at:      0     107     830     929    1205    1974 
   Size:        107     723      99     276     769

  Fragments arranged by size: 

                 769     723     276     107      99
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     260     292    1266    1298    1974 
   Size:        260      32     974      32     676

  Fragments arranged by size: 

                 974     676     260      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     344    1974 
   Size:        344    1630
 
MslI CAynn'nnrTG

Cuts at:      0     451     878    1157    1902    1974 
   Size:        451     427     279     745      72

  Fragments arranged by size: 

                 745     451     427     279      72
 
MspA1I CmG'CkG

Cuts at:      0      90    1974 
   Size:         90    1884
 
NdeI CA'TA_TG

Cuts at:      0    1899    1974 
   Size:       1899      75
 
NsiI A_TGCA'T

Cuts at:      0    1905    1974 
   Size:       1905      69
 
NspI r_CATG'y

Cuts at:      0    1907    1974 
   Size:       1907      67
 
PasI CC'CwG_GG

Cuts at:      0       7    1974 
   Size:          7    1967
 
PmlI CAC'GTG

Cuts at:      0     577    1974 
   Size:        577    1397
 
PpuMI rG'GwC_Cy

Cuts at:      0     107     830     929    1205    1974 
   Size:        107     723      99     276     769

  Fragments arranged by size: 

                 769     723     276     107      99
 
PstI C_TGCA'G

Cuts at:      0     373    1974 
   Size:        373    1601
 
PvuII CAG'CTG

Cuts at:      0      90    1974 
   Size:         90    1884
 
SacI G_AGCT'C

Cuts at:      0    1687    1974 
   Size:       1687     287
 
SfcI C'TryA_G

Cuts at:      0     369    1974 
   Size:        369    1605
 
SphI G_CATG'C

Cuts at:      0    1907    1974 
   Size:       1907      67
 
StuI AGG'CCT

Cuts at:      0     664    1084    1420    1924    1974 
   Size:        664     420     336     504      50

  Fragments arranged by size: 

                 664     504     420     336      50
 
StyI C'CwwG_G

Cuts at:      0     240    1718    1974 
   Size:        240    1478     256

  Fragments arranged by size: 

                1478     256     240
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1039    1189    1291    1459    1724    1870    1974 
   Size:       1039     150     102     168     265     146     104

  Fragments arranged by size: 

                1039     265     168     150     146     104     102
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     346     378    1084    1116    1504    1536    1924    1956 
   Size:        346      32     706      32     388      32     388      32

Cuts at:   1956    1974 
   Size:         18

  Fragments arranged by size: 

                 706     388     388     346      32      32      32      32
                  18
 
XbaI T'CTAG_A

Cuts at:      0     229     417    1974 
   Size:        229     188    1557

  Fragments arranged by size: 

                1557     229     188
 
XmnI GAAnn'nnTTC

Cuts at:      0     916    1000    1252    1336    1420    1756    1974 
   Size:        916      84     252      84      84     336     218

  Fragments arranged by size: 

                 916     336     252     218      84      84      84
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AleI    AlwNI    BamHI    BanII     BbsI
    BfrBI     BlpI    BmgBI     BmrI     BpmI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI   BstEII
    BstXI    BstYI     BtsI    CspCI   DraIII  EcoICRI  Eco57MI EcoO109I
    Hin4I     MmeI     MslI   MspA1I     NdeI     NsiI     NspI     PasI
     PmlI    PpuMI     PstI    PvuII     SacI     SfcI     SphI     StuI
     StyI    TaqII     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII Bme1580I     BmtI     BplI   Bpu10I    BpuEI
    BsaBI    BsiEI    BsiWI    BspHI    BspMI    BsrGI   BssHII    BstBI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI     DraI     DrdI     EaeI
     EagI     EarI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     PacI     PciI    PflMI     PfoI     PmeI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI
     SwaI     TatI  Tth111I     XcmI     XhoI     XmaI     ZraI
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