Restriction map for pF1KB8390
 (Linear) (Six-base) MAPSORT of: KIBB8390.seq  Check: 2821  from: 1  to: 1194



KIBB8390.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     156     308     529     956    1046    1194 
   Size:        156     152     221     427      90     148

  Fragments arranged by size: 

                 427     221     156     152     148      90
 
AatII G_ACGT'C

Cuts at:      0     931    1194 
   Size:        931     263
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     958    1194 
   Size:        958     236
 
AfeI AGC'GCT

Cuts at:      0    1063    1090    1194 
   Size:       1063      27     104

  Fragments arranged by size: 

                1063     104      27
 
AflIII A'CryG_T

Cuts at:      0     887    1194 
   Size:        887     307
 
ApaI G_GGCC'C

Cuts at:      0     648     730    1194 
   Size:        648      82     464

  Fragments arranged by size: 

                 648     464      82
 
AvaI C'yCGr_G

Cuts at:      0     396     856    1075    1194 
   Size:        396     460     219     119

  Fragments arranged by size: 

                 460     396     219     119
 
BanI G'GyrC_C

Cuts at:      0     147     168     586     631     809     850    1034    1194 
   Size:        147      21     418      45     178      41     184     160

  Fragments arranged by size: 

                 418     184     178     160     147      45      41      21
            
 
BanII G_rGCy'C

Cuts at:      0     193     648     730    1194 
   Size:        193     455      82     464

  Fragments arranged by size: 

                 464     455     193      82
 
BbeI G_GCGC'C

Cuts at:      0     151     590     635     854    1194 
   Size:        151     439      45     219     340

  Fragments arranged by size: 

                 439     340     219     151      45
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     298     931    1194 
   Size:        298     633     263

  Fragments arranged by size: 

                 633     298     263
 
BbvCI CC'TCA_GC

Cuts at:      0    1157    1194 
   Size:       1157      37
 
BglI GCCn_nnn'nGGC

Cuts at:      0      10     128     137     629     676     790     859    1194 
   Size:         10     118       9     492      47     114      69     335

  Fragments arranged by size: 

                 492     335     118     114      69      47      10       9
            
 
Bme1580I G_kGCm'C

Cuts at:      0     173     443     648     730    1194 
   Size:        173     270     205      82     464

  Fragments arranged by size: 

                 464     270     205     173      82
 
BmgBI CAC'GTC

Cuts at:      0    1122    1194 
   Size:       1122      72
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     183    1194 
   Size:         31     152    1011

  Fragments arranged by size: 

                1011     152      31
 
Bpu10I CC'TnA_GC

Cuts at:      0    1157    1194 
   Size:       1157      37
 
BsaAI yAC'GTr

Cuts at:      0     438     888     897    1194 
   Size:        438     450       9     297

  Fragments arranged by size: 

                 450     438     297       9
 
BsaHI Gr'CG_yC

Cuts at:      0     148     283     587     632     851     928    1194 
   Size:        148     135     304      45     219      77     266

  Fragments arranged by size: 

                 304     266     219     148     135      77      45
 
BsaWI w'CCGG_w

Cuts at:      0      20    1194 
   Size:         20    1174
 
BseYI C'CCAG_C

Cuts at:      0      61     186     268    1194 
   Size:         61     125      82     926

  Fragments arranged by size: 

                 926     125      82      61
 
BsiEI CG_ry'CG

Cuts at:      0     680     695    1194 
   Size:        680      15     499

  Fragments arranged by size: 

                 680     499      15
 
Bsp1286I G_dGCh'C

Cuts at:      0     173     193     443     648     730    1194 
   Size:        173      20     250     205      82     464

  Fragments arranged by size: 

                 464     250     205     173      82      20
 
BspEI T'CCGG_A

Cuts at:      0      20    1194 
   Size:         20    1174
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     156     308     491     529     956    1046    1194 
   Size:        156     152     183      38     427      90     148

  Fragments arranged by size: 

                 427     183     156     152     148      90      38
 
BsrDI GCAATG_nn'

Cuts at:      0     100    1194 
   Size:        100    1094
 
BsrFI r'CCGG_y

Cuts at:      0     231     378    1194 
   Size:        231     147     816

  Fragments arranged by size: 

                 816     231     147
 
BssHII G'CGCG_C

Cuts at:      0     425    1194 
   Size:        425     769
 
BssSI C'ACGA_G

Cuts at:      0     325    1194 
   Size:        325     869
 
BtgI C'CryG_G

Cuts at:      0       5     214     562     797    1194 
   Size:          5     209     348     235     397

  Fragments arranged by size: 

                 397     348     235     209       5
 
BtsI GCAGTG_nn'

Cuts at:      0     250    1194 
   Size:        250     944
 
EaeI y'GGCC_r

Cuts at:      0       2      11     107     129     677     692     794     800 
   Size:          2       9      96      22     548      15     102       6

Cuts at:    800    1194 
   Size:        394

  Fragments arranged by size: 

                 548     394     102      96      22      15       9       6
                   2
 
EagI C'GGCC_G

Cuts at:      0     677     692    1194 
   Size:        677      15     502

  Fragments arranged by size: 

                 677     502      15
 
EarI CTCTTCn'nnn_

Cuts at:      0     211     997    1194 
   Size:        211     786     197

  Fragments arranged by size: 

                 786     211     197
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     482     877    1194 
   Size:        482     395     317

  Fragments arranged by size: 

                 482     395     317
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     183     958    1194 
   Size:         31     152     775     236

  Fragments arranged by size: 

                 775     236     152      31
 
EcoO109I rG'GnC_Cy

Cuts at:      0     599     644     645     783    1188    1194 
   Size:        599      45       1     138     405       6

  Fragments arranged by size: 

                 599     405     138      45       6       1
 
FspI TGC'GCA

Cuts at:      0     323     743    1099    1194 
   Size:        323     420     356      95

  Fragments arranged by size: 

                 420     356     323      95
 
HaeII r_GCGC'y

Cuts at:      0      68     151     590     635     854    1065    1092    1194 
   Size:         68      83     439      45     219     211      27     102

  Fragments arranged by size: 

                 439     219     211     102      83      68      45      27
            
 
KasI G'GCGC_C

Cuts at:      0     147     586     631     850    1194 
   Size:        147     439      45     219     344

  Fragments arranged by size: 

                 439     344     219     147      45
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     503     713    1194 
   Size:        503     210     481

  Fragments arranged by size: 

                 503     481     210
 
MspA1I CmG'CkG

Cuts at:      0       7     422     682     818     824    1032    1194 
   Size:          7     415     260     136       6     208     162

  Fragments arranged by size: 

                 415     260     208     162     136       7       6
 
NaeI GCC'GGC

Cuts at:      0     233    1194 
   Size:        233     961
 
NarI GG'CG_CC

Cuts at:      0     148     587     632     851    1194 
   Size:        148     439      45     219     343

  Fragments arranged by size: 

                 439     343     219     148      45
 
NgoMIV G'CCGG_C

Cuts at:      0     231    1194 
   Size:        231     963
 
NotI GC'GGCC_GC

Cuts at:      0     677     692    1194 
   Size:        677      15     502

  Fragments arranged by size: 

                 677     502      15
 
NruI TCG'CGA

Cuts at:      0    1114    1194 
   Size:       1114      80
 
NspI r_CATG'y

Cuts at:      0     231    1194 
   Size:        231     963
 
PfoI T'CCnGG_A

Cuts at:      0      47     612    1194 
   Size:         47     565     582

  Fragments arranged by size: 

                 582     565      47
 
PmlI CAC'GTG

Cuts at:      0     888     897    1194 
   Size:        888       9     297

  Fragments arranged by size: 

                 888     297       9
 
PpuMI rG'GwC_Cy

Cuts at:      0    1188    1194 
   Size:       1188       6
 
PspOMI G'GGCC_C

Cuts at:      0     644     726    1194 
   Size:        644      82     468

  Fragments arranged by size: 

                 644     468      82
 
PspXI vC'TCGA_Gb

Cuts at:      0     396    1194 
   Size:        396     798
 
PstI C_TGCA'G

Cuts at:      0      38     167     305     776    1031    1194 
   Size:         38     129     138     471     255     163

  Fragments arranged by size: 

                 471     255     163     138     129      38
 
PvuII CAG'CTG

Cuts at:      0     422    1194 
   Size:        422     772
 
SacII CC_GC'GG

Cuts at:      0       8    1194 
   Size:          8    1186
 
SapI GCTCTTCn'nnn_

Cuts at:      0     211     997    1194 
   Size:        211     786     197

  Fragments arranged by size: 

                 786     211     197
 
SbfI CC_TGCA'GG

Cuts at:      0     167    1194 
   Size:        167    1027
 
SfcI C'TryA_G

Cuts at:      0      34     117     163     301     772    1027    1194 
   Size:         34      83      46     138     471     255     167

  Fragments arranged by size: 

                 471     255     167     138      83      46      34
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      10    1194 
   Size:         10    1184
 
SfoI GGC'GCC

Cuts at:      0     149     588     633     852    1194 
   Size:        149     439      45     219     342

  Fragments arranged by size: 

                 439     342     219     149      45
 
SmlI C'TyrA_G

Cuts at:      0     396    1194 
   Size:        396     798
 
StuI AGG'CCT

Cuts at:      0      40     716    1194 
   Size:         40     676     478

  Fragments arranged by size: 

                 676     478      40
 
StyI C'CwwG_G

Cuts at:      0     779    1194 
   Size:        779     415
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     552    1194 
   Size:        552     642
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     786    1194 
   Size:        786     408
 
XhoI C'TCGA_G

Cuts at:      0     396    1194 
   Size:        396     798
 
XmnI GAAnn'nnTTC

Cuts at:      0    1006    1194 
   Size:       1006     188
 
ZraI GAC'GTC

Cuts at:      0     929    1194 
   Size:        929     265
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI   AflIII     ApaI     AvaI     BanI
    BanII     BbeI     BbsI    BbvCI     BglI Bme1580I    BmgBI     BpmI
   Bpu10I    BsaAI    BsaHI    BsaWI    BseYI    BsiEI Bsp1286I    BspEI
    BspMI    BsrDI    BsrFI   BssHII    BssSI     BtgI     BtsI     EaeI
     EagI     EarI     EciI  Eco57MI EcoO109I     FspI    HaeII     KasI
     MmeI   MspA1I     NaeI     NarI   NgoMIV     NotI     NruI     NspI
     PfoI     PmlI    PpuMI   PspOMI    PspXI     PstI    PvuII    SacII
     SapI     SbfI     SfcI     SfiI     SfoI     SmlI     StuI     StyI
    TaqII     XcmI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI     AlfI
     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
     BmrI     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaXI    BseRI
     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI    BsrBI    BsrGI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI    FspAI    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MscI     MslI     NcoI     NdeI     NheI
     NsiI     PacI     PasI     PciI    PflMI     PmeI     PpiI    PshAI
     PsiI     PsrI     PvuI    RsrII     SacI     SalI    SanDI     ScaI
    SexAI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TatI     TstI  Tth111I     XbaI     XmaI
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