Restriction map for pF1KB8374
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BsaAI       BseAI       BsiWI       
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstUI       BstXI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraI        DraIII      
DsaI        Eam1105I    Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HgaI        HindIII     HpaI        KasI        
KpnI        KspI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AatII        1300  GACGTC               GACGT^C
AccI         1339  GTCTAC               GT^[AC][GT]AC
AcyI         1300  GACGTC               G[GA]^CG[CT]C
AlwNI        1100  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI        47  AGCGCT               AGC^GCT
Asp700I       130  GAACCCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1032  CTCGGG               C^[CT]CG[GA]G
BsaBI         151  GATCTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1300  GACGTC               G[GA]^CG[CT]C
BsaI          454  GAGACC               GGTCTC(1/5)
BsiEI         717  CGGCCG               CG[GA][CT]^CG
BsmBI        1298  GAGACG               CGTCTC(1/5)
BsmI         1152  GAATGC               GAATGC(1/-1)
BsoBI        1032  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     1247  TGTACA               T^GTACA
BspMI        1369  GCAGGT               ACCTGC(4/8)
BsrBI        1519  CCGCTC               CCGCTC(-3/-3)
BsrGI        1247  TGTACA               T^GTACA
BstYI         617  AGATCC               [GA]^GATC[CT]
DrdI         1480  GACTGGACTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          717  CGGCCG               C^GGCCG
EclXI         717  CGGCCG               C^GGCCG
Eco47III       47  AGCGCT               AGC^GCT
Eco52I        717  CGGCCG               C^GGCCG
Hin1I        1300  GACGTC               G[GA]^CG[CT]C
HincII        594  GTTGAC               GT[CT]^[GA]AC
HindII        594  GTTGAC               GT[CT]^[GA]AC
MamI          151  GATCTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          617  AGATCC               [GA]^GATC[CT]
NspI         1211  ACATGC               [GA]CATG^[CT]
SspBI        1247  TGTACA               T^GTACA
XhoII         617  AGATCC               [GA]^GATC[CT]
XmnI          130  GAACCCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcsI          634  GAATTT               [GA]^AATT[CT]
             1829  AAATTC              
AlwI          618  GATCC                GGATC(4/5)
              156  GGATC               
ApaI         1437  GGGCCC               GGGCC^C
             1706  GGGCCC              
ApoI          634  GAATTT               [GA]^AATT[CT]
             1829  AAATTC              
AspHI         258  GAGCAC               G[AT]GC[AT]^C
              343  GTGCTC              
BalI          528  TGGCCA               TGG^CCA
             1667  TGGCCA              
BfaI           31  CTAG                 C^TAG
              220  CTAG                
BlpI           25  GCTGAGC              GC^T[ACGT]AGC
               50  GCTTAGC             
Bpu1102I       25  GCTGAGC              GC^T[ACGT]AGC
               50  GCTTAGC             
BsgI         1106  CTGCAC               GTGCAG(16/14)
             1081  GTGCAG              
BsiHKAI       258  GAGCAC               G[AT]GC[AT]^C
              343  GTGCTC              
BstEII        405  GGTCACC              G^GT[ACGT]ACC
             1393  GGTCACC             
BstPI         405  GGTCACC              G^GT[ACGT]ACC
             1393  GGTCACC             
CelII          25  GCTGAGC              GC^T[ACGT]AGC
               50  GCTTAGC             
EcoO65I       405  GGTCACC              G^GT[ACGT]ACC
             1393  GGTCACC             
HaeII          47  AGCGCT               [GA]GCGC^[CT]
             1613  GGCGCT              
MaeI           31  CTAG                 C^TAG
              220  CTAG                
MluNI         528  TGGCCA               TGG^CCA
             1667  TGGCCA              
MscI          528  TGGCCA               TGG^CCA
             1667  TGGCCA              
PstI          319  CTGCAG               CTGCA^G
              352  CTGCAG              
PvuII         349  CAGCTG               CAG^CTG
             1100  CAGCTG              
TfiI          517  GAATC                G^A[AT]TC
              691  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BanII       
BbsI        BbvI        BmyI        BpmI        BpuAI       
BsaJI       BsaWI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
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