(Linear) (Six-base) MAPSORT of: KIBB8373.seq  Check: 8235  from: 1  to: 513



KIBB8373.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     513 
   Size:        349     164
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     388     513 
   Size:        388     125
 
AlwNI CAG_nnn'CTG

Cuts at:      0      28     513 
   Size:         28     485
 
ApoI r'AATT_y

Cuts at:      0       7     323     513 
   Size:          7     316     190

  Fragments arranged by size: 

                 316     190       7
 
BclI T'GATC_A

Cuts at:      0      59     513 
   Size:         59     454
 
BglII A'GATC_T

Cuts at:      0     117     513 
   Size:        117     396
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     513 
   Size:        292     221
 
BsaWI w'CCGG_w

Cuts at:      0     195     513 
   Size:        195     318
 
BseYI C'CCAG_C

Cuts at:      0      21     513 
   Size:         21     492
 
BsiHKAI G_wGCw'C

Cuts at:      0     146     188     513 
   Size:        146      42     325

  Fragments arranged by size: 

                 325     146      42
 
Bsp1286I G_dGCh'C

Cuts at:      0     146     188     513 
   Size:        146      42     325

  Fragments arranged by size: 

                 325     146      42
 
BspEI T'CCGG_A

Cuts at:      0     195     513 
   Size:        195     318
 
BsrDI GCAATG_nn'

Cuts at:      0     356     513 
   Size:        356     157
 
BstYI r'GATC_y

Cuts at:      0     117     513 
   Size:        117     396
 
BtgI C'CryG_G

Cuts at:      0     247     513 
   Size:        247     266
 
BtsI GCAGTG_nn'

Cuts at:      0      26      48     156     513 
   Size:         26      22     108     357

  Fragments arranged by size: 

                 357     108      26      22
 
EarI CTCTTCn'nnn_

Cuts at:      0     420     446     513 
   Size:        420      26      67

  Fragments arranged by size: 

                 420      67      26
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0       7     513 
   Size:          7     506
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     513 
   Size:        349     164
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     449     504     513 
   Size:        449      55       9

  Fragments arranged by size: 

                 449      55       9
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     439     513 
   Size:        439      74
 
NspI r_CATG'y

Cuts at:      0     375     513 
   Size:        375     138
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     162     513 
   Size:        162     351
 
PstI C_TGCA'G

Cuts at:      0      43     367     513 
   Size:         43     324     146

  Fragments arranged by size: 

                 324     146      43
 
ScaI AGT'ACT

Cuts at:      0     487     513 
   Size:        487      26
 
SfcI C'TryA_G

Cuts at:      0      39     363     513 
   Size:         39     324     150

  Fragments arranged by size: 

                 324     150      39
 
SmlI C'TyrA_G

Cuts at:      0     271     513 
   Size:        271     242
 
SphI G_CATG'C

Cuts at:      0     375     513 
   Size:        375     138
 
TatI w'GTAC_w

Cuts at:      0     227     485     513 
   Size:        227     258      28

  Fragments arranged by size: 

                 258     227      28
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApoI     BclI    BglII    BpuEI    BsaWI
    BseYI  BsiHKAI Bsp1286I    BspEI    BsrDI    BstYI     BtgI     BtsI
     EarI     EciI  Eco57MI     FalI     MmeI     NspI    PflMI     PstI
     ScaI     SfcI     SmlI     SphI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI