Restriction map for pF1KB8369
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvrII       BalI        BamHI       BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraII       DraIII      EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        HindIII     
HphI        KpnI        MaeIII      MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SalI        SapI        
ScaI        SexAI       SfaNI       SfiI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
SspBI       SspI        StuI        StyI        SwaI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII         584  GACGTC               GACGT^C
AccII         275  CGCG                 CG^CG
AcsI          180  GAATTT               [GA]^AATT[CT]
AfaI          219  GTAC                 GT^AC
AluI          247  AGCT                 AG^CT
AlwI          406  GATCC                GGATC(4/5)
ApoI          180  GAATTT               [GA]^AATT[CT]
Asp700I        90  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          64  GAGCAC               G[AT]GC[AT]^C
AviII         442  TGCGCA               TGC^GCA
BbeI          345  GGCGCC               GGCGC^C
BbsI          581  GAAGAC               GAAGAC(2/6)
BpmI           37  CTGGAG               CTGGAG(16/14)
BpuAI         581  GAAGAC               GAAGAC(2/6)
BsiHKAI        64  GAGCAC               G[AT]GC[AT]^C
BsmAI         149  GAGAC                GTCTC(1/5)
BsmFI         128  GGGAC                GGGAC(10/14)
BspMI         462  ACCTGC               ACCTGC(4/8)
BsrBI          40  GAGCGG               CCGCTC(-3/-3)
BsrDI         438  GCAATG               GCAATG(2/0)
BstUI         275  CGCG                 CG^CG
DpnII         406  GATC                 ^GATC
DrdI          402  GACTGATCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          274  CCGCGG               C^C[GA][CT]GG
FspI          442  TGCGCA               TGC^GCA
HaeII         345  GGCGCC               [GA]GCGC^[CT]
HincII        120  GTTAAC               GT[CT]^[GA]AC
HindII        120  GTTAAC               GT[CT]^[GA]AC
HpaI          120  GTTAAC               GTT^AAC
KasI          345  GGCGCC               G^GCGCC
KspI          274  CCGCGG               CCGC^GG
MboI          406  GATC                 ^GATC
MvnI          275  CGCG                 CG^CG
NaeI          303  GCCGGC               GCC^GGC
NarI          345  GGCGCC               GG^CGCC
NdeII         406  GATC                 ^GATC
NgoMIV        303  GCCGGC               G^CCGGC
NlaIII        153  CATG                 CATG^
RsaI          219  GTAC                 GT^AC
SacII         274  CCGCGG               CCGC^GG
Sau3AI        406  GATC                 ^GATC
SbfI           83  CCTGCAGG             CCTGCA^GG
SfoI          345  GGCGCC               GGC^GCC
SmaI          125  CCCGGG               CCC^GGG
Sse8387I       83  CCTGCAGG             CCTGCA^GG
TaqI          185  TCGA                 T^CGA
TspRI         142  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       185  TCGA                 T^CGA
XmaCI         125  CCCGGG               C^CCGGG
XmaI          125  CCCGGG               C^CCGGG
XmnI           90  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AvaI          125  CCCGGG               C^[CT]CG[GA]G
              253  CCCGAG              
AvaII         108  GGACC                G^G[AT]CC
              393  GGACC               
BanII          27  GAGCCC               G[GA]GC[CT]^C
              359  GGGCTC              
BbvI          245  GCAGC                GCAGC(8/12)
              326  GCAGC               
BfaI           32  CTAG                 C^TAG
              208  CTAG                
BglI           52  GCCAGGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              317  GCCAGGATGGC         
BsoBI         125  CCCGGG               C^[CT]CG[GA]G
              253  CCCGAG              
BsrFI         303  GCCGGC               [GA]^CCGG[CT]
              541  GCCGGT              
BstF5I         44  GGATG                GGATG(2/0)
              321  GGATG               
Cfr10I        303  GCCGGC               [GA]^CCGG[CT]
              541  GCCGGT              
DdeI          374  CTGAG                C^T[ACGT]AG
              577  CTCAG               
FauI          273  CCCGC                CCCGC(4/6)
              329  GCGGG               
FokI           44  GGATG                GGATG(9/13)
              321  GGATG               
HgaI           49  GACGC                GACGC(5/10)
              558  GACGC               
HinfI         195  GAATC                G^A[ACGT]TC
              475  GATTC               
MaeI           32  CTAG                 C^TAG
              208  CTAG                
MaeII           8  ACGT                 A^CGT
              585  ACGT                
MseI          121  TTAA                 T^TAA
              352  TTAA                
MspA1I        274  CCGCGG               C[AC]G^C[GT]G
              327  CAGCGG              
PstI           84  CTGCAG               CTGCA^G
              144  CTGCAG              
TfiI          195  GAATC                G^A[AT]TC
              475  GATTC               
Tru9I         121  TTAA                 T^TAA
              352  TTAA                
TseI          245  GCAGC                G^C[AT]GC
              326  GCAGC               
Tsp509I       181  AATT                 ^AATT
              354  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AciI        AcyI        BanI        BcnI        BmyI        
BsaHI       BsaJI       BsiYI       BslI        Bsp1286I    
BssKI       BstNI       Cac8I       CfoI        Cfr13I      
EarI        EcoRII      Fnu4HI      HaeIII      HapII       
HhaI        Hin1I       HinP1I      HpaII       ItaI        
Ksp632I     MboII       MnlI        MspI        MvaI        
MwoI        NciI        NlaIV       Sau96I      ScrFI       
SfcI        
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