Restriction map for pF1KB8357
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AflIII      
AgeI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AviII       AvrII       
BanI        BbeI        BbrPI       BbsI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BpuAI       BsaAI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CfoI        ClaI        CpoI        
DraI        DraIII      EagI        EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        HhaI        HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     MaeI        MfeI        MluI        MroI        
MseI        MunI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tru9I       Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccI           52  GTCTAC               GT^[AC][GT]AC
AccII          68  CGCG                 CG^CG
AcsI          400  GAATTT               [GA]^AATT[CT]
AhdI          181  GACAGGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          400  GAATTT               [GA]^AATT[CT]
AspEI         181  GACAGGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          524  CCCGAG               C^[CT]CG[GA]G
BalI          685  TGGCCA               TGG^CCA
BamHI         631  GGATCC               G^GATCC
BanII         196  GAGCTC               G[GA]GC[CT]^C
BbvCI         135  GCTGAGG              CCTCAGC(-5/-2)
BcgI          263  GCAGGTACTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          213  CCCGG                CC^[GC]GG
BlpI          552  GCTGAGC              GC^T[ACGT]AGC
BpmI          175  CTGGAG               CTGGAG(16/14)
Bpu1102I      552  GCTGAGC              GC^T[ACGT]AGC
BsaBI         276  GATCCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI           4  GAGGAG               GAGGAG(10/8)
BsmAI         722  GAGAC                GTCTC(1/5)
BsoBI         524  CCCGAG               C^[CT]CG[GA]G
BspMI         263  GCAGGT               ACCTGC(4/8)
BsrBI           9  GAGCGG               CCGCTC(-3/-3)
BsrDI         362  CATTGC               GCAATG(2/0)
BsrFI         131  GCCGGC               [GA]^CCGG[CT]
BstF5I         60  CATCC                GGATG(2/0)
BstUI          68  CGCG                 CG^CG
BstYI         631  GGATCC               [GA]^GATC[CT]
CelII         552  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        131  GCCGGC               [GA]^CCGG[CT]
DrdI           43  GACGTCCACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      181  GACAGGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         568  CCTCCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       429  CCAAGG               C^C[AT][AT]GG
FokI           60  CATCC                GGATG(9/13)
KspI           67  CCGCGG               CCGC^GG
MaeIII        250  GTGAC                ^GT[ACGT]AC
MamI          276  GATCCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          631  GGATCC               [GA]^GATC[CT]
MluNI         685  TGGCCA               TGG^CCA
MscI          685  TGGCCA               TGG^CCA
MslI          234  CACAGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           68  CGCG                 CG^CG
NaeI          131  GCCGGC               GCC^GGC
NciI          213  CCCGG                CC^[GC]GG
NgoMIV        131  GCCGGC               G^CCGGC
SacI          196  GAGCTC               GAGCT^C
SacII          67  CCGCGG               CCGC^GG
StyI          429  CCAAGG               C^C[AT][AT]GG
TaqI          284  TCGA                 T^CGA
Tsp45I        250  GTGAC                ^GT[GC]AC
TthHB8I       284  TCGA                 T^CGA
XcmI          766  CCAGGCACAGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         631  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AatII          43  GACGTC               GACGT^C
              186  GACGTC              
AcyI           43  GACGTC               G[GA]^CG[CT]C
              186  GACGTC              
BsaHI          43  GACGTC               G[GA]^CG[CT]C
              186  GACGTC              
BsmFI         153  GGGAC                GGGAC(10/14)
              329  GGGAC               
DraII         209  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              432  AGGCCCT             
DsaI           67  CCGCGG               C^C[GA][CT]GG
               81  CCGTGG              
EaeI          129  TGGCCG               [CT]^GGCC[GA]
              685  TGGCCA              
EcoO109I      209  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              432  AGGCCCT             
FauI           11  GCGGG                CCCGC(4/6)
               69  GCGGG               
Hin1I          43  GACGTC               G[GA]^CG[CT]C
              186  GACGTC              
HphI          337  GGTGA                GGTGA(8/7)
              384  GGTGA               
NlaIII        122  CATG                 CATG^
              492  CATG                
PleI           19  GAGTC                GAGTC(4/5)
              527  GAGTC               
SfcI           87  CTACAG               C^T[GA][CT]AG
              737  CTACAG              
TfiI          256  GATTC                G^A[AT]TC
              519  GAATC               
Tsp509I       318  AATT                 ^AATT
              401  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaII       BbvI        BmyI        BsaJI       BsiHKAI     
BsiYI       BslI        Bsp1286I    BsrI        BssKI       
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
EcoRII      Fnu4HI      HaeIII      HapII       HinfI       
HpaII       ItaI        MaeII       MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIV       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TseI        TspRI       
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