Restriction map for pF1KB8355
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BciVI       
BclI        BfrI        BglII       BlnI        BsiEI       
BsiWI       BsmBI       BsmI        BspDI       BspHI       
BspLU11I    BsrDI       BssHII      Bst1107I    BstBI       
BstYI       BstZ17I     ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRI       EcoRV       EcoT22I     
FbaI        FspI        HpaI        KpnI        KspI        
MfeI        MflI        MluI        MunI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        SspI        
SwaI        TfiI        Tsp509I     Tth111I     XbaI        
XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI          340  GTCGAC               GT^[AC][GT]AC
AccIII       1554  TCCGGA               T^CCGGA
BalI           35  TGGCCA               TGG^CCA
BbrPI         307  CACGTG               CAC^GTG
BbvCI        1694  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1578  CGAAGGCTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1156  CTAG                 C^TAG
BglI         1183  GCCCCTCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1051  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1051  GCTGAGC              GC^T[ACGT]AGC
BsaBI        1887  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        1554  TCCGGA               T^CCGGA
BsgI          439  CTGCAC               GTGCAG(16/14)
Bsp1407I       98  TGTACA               T^GTACA
BspEI        1554  TCCGGA               T^CCGGA
BspMI        1739  ACCTGC               ACCTGC(4/8)
BsrFI        1198  GCCGGC               [GA]^CCGG[CT]
BsrGI          98  TGTACA               T^GTACA
BssSI          79  CTCGTG               CACGAG(-5/-1)
BstAPI        441  GCACCGACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        1051  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1198  GCCGGC               [GA]^CCGG[CT]
EaeI           35  TGGCCA               [CT]^GGCC[GA]
FseI         1197  GGCCGGCC             GGCCGG^CC
HincII        340  GTCGAC               GT[CT]^[GA]AC
HindII        340  GTCGAC               GT[CT]^[GA]AC
HindIII       130  AAGCTT               A^AGCTT
MaeI         1156  CTAG                 C^TAG
MamI         1887  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI          35  TGGCCA               TGG^CCA
MroI         1554  TCCGGA               T^CCGGA
MscI           35  TGGCCA               TGG^CCA
MseI          530  TTAA                 T^TAA
MslI          784  CATCGGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI         1198  GCCGGC               GCC^GGC
NdeI          104  CATATG               CA^TATG
NgoMIV       1198  GCCGGC               G^CCGGC
NspI         1897  ACATGC               [GA]CATG^[CT]
PflMI          13  CCATGGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         307  CACGTG               CAC^GTG
PmlI          307  CACGTG               CAC^GTG
PpuMI        1774  GGGACCC              [GA]G^G[AT]CC[CT]
SacI         1837  GAGCTC               GAGCT^C
SalI          340  GTCGAC               G^TCGAC
SbfI           86  CCTGCAGG             CCTGCA^GG
SmaI         1238  CCCGGG               CCC^GGG
Sse8387I       86  CCTGCAGG             CCTGCA^GG
SspBI          98  TGTACA               T^GTACA
Tru9I         530  TTAA                 T^TAA
Van91I         13  CCATGGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        1238  CCCGGG               C^CCGGG
XmaI         1238  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AocI         1186  CCTCAGG              CC^T[ACGT]AGG
             1219  CCTCAGG             
ApaI         1067  GGGCCC               GGGCC^C
             1487  GGGCCC              
AvaI         1238  CCCGGG               C^[CT]CG[GA]G
             1503  CTCGGG              
AvaII        1341  GGTCC                G^G[AT]CC
             1775  GGACC               
BbeI          990  GGCGCC               GGCGC^C
             1230  GGCGCC              
BbsI          370  GAAGAC               GAAGAC(2/6)
              498  GAAGAC              
BpuAI         370  GAAGAC               GAAGAC(2/6)
              498  GAAGAC              
BsaAI         244  TACGTG               [CT]AC^GT[GA]
              307  CACGTG              
BsaWI         980  ACCGGA               [AT]^CCGG[AT]
             1554  TCCGGA              
BsmFI         171  GGGAC                GGGAC(10/14)
             1774  GGGAC               
BsoBI        1238  CCCGGG               C^[CT]CG[GA]G
             1503  CTCGGG              
BstXI         302  CCAGCCACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              359  CCAGCAGCGTGG        
Bsu36I       1186  CCTCAGG              CC^T[ACGT]AGG
             1219  CCTCAGG             
Eco81I       1186  CCTCAGG              CC^T[ACGT]AGG
             1219  CCTCAGG             
EcoNI        1071  CCTGGATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1521  CCTGGCAGAGG         
KasI          990  GGCGCC               G^GCGCC
             1230  GGCGCC              
NarI          990  GGCGCC               GG^CGCC
             1230  GGCGCC              
NcoI           13  CCATGG               C^CATGG
             1205  CCATGG              
SapI          568  GAAGAGC              GCTCTTC(1/4)
             1834  GAAGAGC             
SfoI          990  GGCGCC               GGC^GCC
             1230  GGCGCC              
SmlI          127  CTCAAG               C^T[CT][GA]AG
             1702  CTTGAG              
StuI           47  AGGCCT               AGG^CCT
             1190  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AspHI       BanI        BanII       BbvI        BcnI        
BmyI        BpmI        BsaHI       BsaI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    BsrBI       BsrI        BssKI       BstEII      
BstF5I      BstNI       BstPI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EarI        EcoO109I    EcoO65I     EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
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