Restriction map for pF1KB8355
 (Linear) (Six-base) MAPSORT of: KIBB8355.seq  Check: 5370  from: 1  to: 1953



KIBB8355.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1748    1953 
   Size:       1748     205
 
AccI GT'mk_AC

Cuts at:      0     341    1953 
   Size:        341    1612
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1782    1953 
   Size:       1782     171
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      41    1953 
   Size:         41    1912
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1255    1287    1953 
   Size:       1255      32     666

  Fragments arranged by size: 

                1255     666      32
 
ApaI G_GGCC'C

Cuts at:      0    1071    1491    1953 
   Size:       1071     420     462

  Fragments arranged by size: 

                1071     462     420
 
AvaI C'yCGr_G

Cuts at:      0    1238    1503    1953 
   Size:       1238     265     450

  Fragments arranged by size: 

                1238     450     265
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1499    1532    1785    1818    1953 
   Size:       1499      33     253      33     135

  Fragments arranged by size: 

                1499     253     135      33      33
 
BanI G'GyrC_C

Cuts at:      0     385     484     990    1230    1530    1953 
   Size:        385      99     506     240     300     423

  Fragments arranged by size: 

                 506     423     385     300     240      99
 
BanII G_rGCy'C

Cuts at:      0      96     479    1018    1071    1145    1217    1484    1491 
   Size:         96     383     539      53      74      72     267       7

Cuts at:   1491    1664    1736    1841    1953 
   Size:        173      72     105     112

  Fragments arranged by size: 

                 539     383     267     173     112     105      96      74
                  72      72      53       7
 
BbeI G_GCGC'C

Cuts at:      0     994    1234    1953 
   Size:        994     240     719

  Fragments arranged by size: 

                 994     719     240
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     377     505    1953 
   Size:        377     128    1448

  Fragments arranged by size: 

                1448     377     128
 
BbvCI CC'TCA_GC

Cuts at:      0    1695    1953 
   Size:       1695     258
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1567    1601    1953 
   Size:       1567      34     352

  Fragments arranged by size: 

                1567     352      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1189    1953 
   Size:       1189     764
 
BlpI GC'TnA_GC

Cuts at:      0    1052    1953 
   Size:       1052     901
 
Bme1580I G_kGCm'C

Cuts at:      0     487    1071    1491    1535    1953 
   Size:        487     584     420      44     418

  Fragments arranged by size: 

                 584     487     420     418      44
 
BmgBI CAC'GTC

Cuts at:      0     345    1953 
   Size:        345    1608
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     267    1909    1953 
   Size:        267    1642      44

  Fragments arranged by size: 

                1642     267      44
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      92    1101    1953 
   Size:         92    1009     852

  Fragments arranged by size: 

                1009     852      92
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      72     411    1011    1456    1732    1865    1953 
   Size:         72     339     600     445     276     133      88

  Fragments arranged by size: 

                 600     445     339     276     133      88      72
 
Bpu10I CC'TnA_GC

Cuts at:      0     314     616    1616    1695    1953 
   Size:        314     302    1000      79     258

  Fragments arranged by size: 

                1000     314     302     258      79
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112    1723    1953 
   Size:        112    1611     230

  Fragments arranged by size: 

                1611     230     112
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     796    1126    1162    1750    1953 
   Size:        796     330      36     588     203

  Fragments arranged by size: 

                 796     588     330     203      36
 
BsaAI yAC'GTr

Cuts at:      0     246     309    1953 
   Size:        246      63    1644

  Fragments arranged by size: 

                1644     246      63
 
BsaBI GATnn'nnATC

Cuts at:      0    1891    1953 
   Size:       1891      62
 
BsaHI Gr'CG_yC

Cuts at:      0     991    1231    1331    1953 
   Size:        991     240     100     622

  Fragments arranged by size: 

                 991     622     240     100
 
BsaWI w'CCGG_w

Cuts at:      0     980    1554    1953 
   Size:        980     574     399

  Fragments arranged by size: 

                 980     574     399
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1804    1834    1953 
   Size:       1804      30     119

  Fragments arranged by size: 

                1804     119      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     598     601     676     718     808     850    1077    1180 
   Size:        598       3      75      42      90      42     227     103

Cuts at:   1180    1225    1720    1828    1831    1942    1945    1953 
   Size:         45     495     108       3     111       3       8

  Fragments arranged by size: 

                 598     495     227     111     108     103      90      75
                  45      42      42       8       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0       4     857    1144    1901    1953 
   Size:          4     853     287     757      52

  Fragments arranged by size: 

                 853     757     287      52       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424    1953 
   Size:        424    1529
 
BsiHKAI G_wGCw'C

Cuts at:      0     251     710    1841    1953 
   Size:        251     459    1131     112

  Fragments arranged by size: 

                1131     459     251     112
 
Bsp1286I G_dGCh'C

Cuts at:      0      96     251     479     487     710    1018    1071    1145 
   Size:         96     155     228       8     223     308      53      74

Cuts at:   1145    1217    1484    1491    1535    1664    1736    1841    1953 
   Size:         72     267       7      44     129      72     105     112

  Fragments arranged by size: 

                 308     267     228     223     155     129     112     105
                  96      74      72      72      53      44       8       7
            
 
BspEI T'CCGG_A

Cuts at:      0    1554    1953 
   Size:       1554     399
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1748    1953 
   Size:       1748     205
 
BsrBI CCG'CTC

Cuts at:      0     591     633     648     672     687    1065    1953 
   Size:        591      42      15      24      15     378     888

  Fragments arranged by size: 

                 888     591     378      42      24      15      15
 
BsrFI r'CCGG_y

Cuts at:      0    1198    1953 
   Size:       1198     755
 
BsrGI T'GTAC_A

Cuts at:      0      98    1953 
   Size:         98    1855
 
BssSI C'ACGA_G

Cuts at:      0      79    1953 
   Size:         79    1874
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     447    1953 
   Size:        447    1506
 
BstEII G'GTnAC_C

Cuts at:      0     778    1080    1515    1953 
   Size:        778     302     435     438

  Fragments arranged by size: 

                 778     438     435     302
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     309     366    1953 
   Size:        309      57    1587

  Fragments arranged by size: 

                1587     309      57
 
Bsu36I CC'TnA_GG

Cuts at:      0    1187    1220    1953 
   Size:       1187      33     733

  Fragments arranged by size: 

                1187     733      33
 
BtgI C'CryG_G

Cuts at:      0      13     479    1205    1310    1334    1953 
   Size:         13     466     726     105      24     619

  Fragments arranged by size: 

                 726     619     466     105      24      13
 
BtsI GCAGTG_nn'

Cuts at:      0     216    1102    1373    1379    1561    1953 
   Size:        216     886     271       6     182     392

  Fragments arranged by size: 

                 886     392     271     216     182       6
 
EaeI y'GGCC_r

Cuts at:      0      35    1953 
   Size:         35    1918
 
EarI CTCTTCn'nnn_

Cuts at:      0     563     734     794    1829    1919    1953 
   Size:        563     171      60    1035      90      34

  Fragments arranged by size: 

                1035     563     171      90      60      34
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1844    1953 
   Size:       1844     109
 
EcoICRI GAG'CTC

Cuts at:      0    1839    1953 
   Size:       1839     114
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      72     411    1011    1456    1732    1782    1865    1953 
   Size:         72     339     600     445     276      50      83      88

  Fragments arranged by size: 

                 600     445     339     276      88      83      72      50
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1075    1525    1953 
   Size:       1075     450     428

  Fragments arranged by size: 

                1075     450     428
 
EcoO109I rG'GnC_Cy

Cuts at:      0     600    1068    1460    1487    1488    1775    1953 
   Size:        600     468     392      27       1     287     178

  Fragments arranged by size: 

                 600     468     392     287     178      27       1
 
FseI GG_CCGG'CC

Cuts at:      0    1202    1953 
   Size:       1202     751
 
HaeII r_GCGC'y

Cuts at:      0     678     994    1234    1953 
   Size:        678     316     240     719

  Fragments arranged by size: 

                 719     678     316     240
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      62      94     922     954    1671    1703    1953 
   Size:         62      32     828      32     717      32     250

  Fragments arranged by size: 

                 828     717     250      62      32      32      32
 
HincII GTy'rAC

Cuts at:      0     342    1953 
   Size:        342    1611
 
HindIII A'AGCT_T

Cuts at:      0     130    1953 
   Size:        130    1823
 
KasI G'GCGC_C

Cuts at:      0     990    1230    1953 
   Size:        990     240     723

  Fragments arranged by size: 

                 990     723     240
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     806    1953 
   Size:        806    1147
 
MscI TGG'CCA

Cuts at:      0      37    1953 
   Size:         37    1916
 
MslI CAynn'nnrTG

Cuts at:      0     788    1953 
   Size:        788    1165
 
MspA1I CmG'CkG

Cuts at:      0     399     511     861     926    1327    1354    1417    1953 
   Size:        399     112     350      65     401      27      63     536

  Fragments arranged by size: 

                 536     401     399     350     112      65      63      27
            
 
NaeI GCC'GGC

Cuts at:      0    1200    1953 
   Size:       1200     753
 
NarI GG'CG_CC

Cuts at:      0     991    1231    1953 
   Size:        991     240     722

  Fragments arranged by size: 

                 991     722     240
 
NcoI C'CATG_G

Cuts at:      0      13    1205    1953 
   Size:         13    1192     748

  Fragments arranged by size: 

                1192     748      13
 
NdeI CA'TA_TG

Cuts at:      0     105    1953 
   Size:        105    1848
 
NgoMIV G'CCGG_C

Cuts at:      0    1198    1953 
   Size:       1198     755
 
NspI r_CATG'y

Cuts at:      0    1901    1953 
   Size:       1901      52
 
PasI CC'CwG_GG

Cuts at:      0    1484    1953 
   Size:       1484     469
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      19    1953 
   Size:         19    1934
 
PmlI CAC'GTG

Cuts at:      0     309    1953 
   Size:        309    1644
 
PpuMI rG'GwC_Cy

Cuts at:      0    1775    1953 
   Size:       1775     178
 
PspOMI G'GGCC_C

Cuts at:      0    1067    1487    1953 
   Size:       1067     420     466

  Fragments arranged by size: 

                1067     466     420
 
PstI C_TGCA'G

Cuts at:      0      30      91     211    1260    1287    1953 
   Size:         30      61     120    1049      27     666

  Fragments arranged by size: 

                1049     666     120      61      30      27
 
PvuII CAG'CTG

Cuts at:      0     511     926    1327    1354    1953 
   Size:        511     415     401      27     599

  Fragments arranged by size: 

                 599     511     415     401      27
 
SacI G_AGCT'C

Cuts at:      0    1841    1953 
   Size:       1841     112
 
SalI G'TCGA_C

Cuts at:      0     340    1953 
   Size:        340    1613
 
SanDI GG'GwC_CC

Cuts at:      0    1775    1953 
   Size:       1775     178
 
SapI GCTCTTCn'nnn_

Cuts at:      0     563    1829    1953 
   Size:        563    1266     124

  Fragments arranged by size: 

                1266     563     124
 
SbfI CC_TGCA'GG

Cuts at:      0      91    1953 
   Size:         91    1862
 
SfcI C'TryA_G

Cuts at:      0      26      87     207    1256    1283    1953 
   Size:         26      61     120    1049      27     670

  Fragments arranged by size: 

                1049     670     120      61      27      26
 
SfoI GGC'GCC

Cuts at:      0     992    1232    1953 
   Size:        992     240     721

  Fragments arranged by size: 

                 992     721     240
 
SmaI CCC'GGG

Cuts at:      0    1240    1953 
   Size:       1240     713
 
SmlI C'TyrA_G

Cuts at:      0     127    1702    1953 
   Size:        127    1575     251

  Fragments arranged by size: 

                1575     251     127
 
StuI AGG'CCT

Cuts at:      0      49    1192    1953 
   Size:         49    1143     761

  Fragments arranged by size: 

                1143     761      49
 
StyI C'CwwG_G

Cuts at:      0      13     874    1205    1847    1953 
   Size:         13     861     331     642     106

  Fragments arranged by size: 

                 861     642     331     106      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     249    1777    1953 
   Size:        249    1528     176

  Fragments arranged by size: 

                1528     249     176
 
TatI w'GTAC_w

Cuts at:      0      98    1953 
   Size:         98    1855
 
XmaI C'CCGG_G

Cuts at:      0    1238    1953 
   Size:       1238     715
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AlfI     AloI     ApaI     AvaI     BaeI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BsrGI    BssSI
   BstAPI   BstEII    BstXI   Bsu36I     BtgI     BtsI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FseI    HaeII    Hin4I
   HincII  HindIII     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NspI     PasI    PflMI     PmlI
    PpuMI   PspOMI     PstI    PvuII     SacI     SalI    SanDI     SapI
     SbfI     SfcI     SfoI     SmaI     SmlI     StuI     StyI    TaqII
     TatI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
    BamHI    BciVI     BclI    BfrBI    BglII     BmtI    BsiEI    BsiWI
     BsmI    BsmBI    BspHI    BsrDI   BssHII    BstBI    BstYI  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI    EcoRI
    EcoRV     FalI     FspI    FspAI     HpaI     KpnI     MfeI     MluI
     NheI     NotI     NruI     NsiI     PacI     PciI     PfoI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI
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