Restriction map for pF1KB8337
 (Linear) (Six-base) MAPSORT of: KIBB8337.seq  Check: 5045  from: 1  to: 1617



KIBB8337.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     615     779     783    1035    1455    1617 
   Size:        615     164       4     252     420     162

  Fragments arranged by size: 

                 615     420     252     164     162       4
 
AflIII A'CryG_T

Cuts at:      0    1571    1617 
   Size:       1571      46
 
AgeI A'CCGG_T

Cuts at:      0     857    1617 
   Size:        857     760
 
AleI CACnn'nnGTG

Cuts at:      0     524    1617 
   Size:        524    1093
 
AlwNI CAG_nnn'CTG

Cuts at:      0     701    1422    1617 
   Size:        701     721     195

  Fragments arranged by size: 

                 721     701     195
 
ApaI G_GGCC'C

Cuts at:      0      97    1617 
   Size:         97    1520
 
ApoI r'AATT_y

Cuts at:      0     665    1169    1617 
   Size:        665     504     448

  Fragments arranged by size: 

                 665     504     448
 
AvaI C'yCGr_G

Cuts at:      0      71    1617 
   Size:         71    1546
 
BamHI G'GATC_C

Cuts at:      0    1505    1617 
   Size:       1505     112
 
BanI G'GyrC_C

Cuts at:      0     241    1617 
   Size:        241    1376
 
BanII G_rGCy'C

Cuts at:      0      97     221    1617 
   Size:         97     124    1396

  Fragments arranged by size: 

                1396     124      97
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     883    1617 
   Size:        883     734
 
BclI T'GATC_A

Cuts at:      0     507    1617 
   Size:        507    1110
 
BglI GCCn_nnn'nGGC

Cuts at:      0      92    1617 
   Size:         92    1525
 
Bme1580I G_kGCm'C

Cuts at:      0      97     244    1617 
   Size:         97     147    1373

  Fragments arranged by size: 

                1373     147      97
 
BmgBI CAC'GTC

Cuts at:      0      54     123    1617 
   Size:         54      69    1494

  Fragments arranged by size: 

                1494      69      54
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     166     683     935    1617 
   Size:        166     517     252     682

  Fragments arranged by size: 

                 682     517     252     166
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     206    1199    1283    1617 
   Size:        151      55     993      84     334

  Fragments arranged by size: 

                 993     334     151      84      55
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     127     507    1617 
   Size:        127     380    1110

  Fragments arranged by size: 

                1110     380     127
 
BsaAI yAC'GTr

Cuts at:      0    1484    1617 
   Size:       1484     133
 
BsaWI w'CCGG_w

Cuts at:      0     857    1009    1093    1499    1617 
   Size:        857     152      84     406     118

  Fragments arranged by size: 

                 857     406     152     118      84
 
BseYI C'CCAG_C

Cuts at:      0     276    1617 
   Size:        276    1341
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1386    1408    1554    1617 
   Size:       1386      22     146      63

  Fragments arranged by size: 

                1386     146      63      22
 
BsiHKAI G_wGCw'C

Cuts at:      0     260    1483    1617 
   Size:        260    1223     134

  Fragments arranged by size: 

                1223     260     134
 
BsmI GAATG_Cn'

Cuts at:      0    1285    1617 
   Size:       1285     332
 
Bsp1286I G_dGCh'C

Cuts at:      0      97     221     244     260    1483    1617 
   Size:         97     124      23      16    1223     134

  Fragments arranged by size: 

                1223     134     124      97      23      16
 
BspHI T'CATG_A

Cuts at:      0     902    1617 
   Size:        902     715
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     177    1617 
   Size:        177    1440
 
BsrDI GCAATG_nn'

Cuts at:      0    1290    1617 
   Size:       1290     327
 
BsrFI r'CCGG_y

Cuts at:      0     857    1617 
   Size:        857     760
 
BsrGI T'GTAC_A

Cuts at:      0     582    1617 
   Size:        582    1035
 
BssSI C'ACGA_G

Cuts at:      0    1237    1617 
   Size:       1237     380
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1457    1617 
   Size:       1457     160
 
BstEII G'GTnAC_C

Cuts at:      0      39    1617 
   Size:         39    1578
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1239    1617 
   Size:       1239     378
 
BstYI r'GATC_y

Cuts at:      0     339     349    1505    1617 
   Size:        339      10    1156     112

  Fragments arranged by size: 

                1156     339     112      10
 
Bsu36I CC'TnA_GG

Cuts at:      0     481    1617 
   Size:        481    1136
 
BtgI C'CryG_G

Cuts at:      0    1428    1617 
   Size:       1428     189
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1104    1139    1272    1307    1617 
   Size:       1104      35     133      35     310

  Fragments arranged by size: 

                1104     310     133      35      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     755     984    1617 
   Size:        755     229     633

  Fragments arranged by size: 

                 755     633     229
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     206     615     779     783    1035    1199    1283 
   Size:        151      55     409     164       4     252     164      84

Cuts at:   1283    1455    1617 
   Size:        172     162

  Fragments arranged by size: 

                 409     252     172     164     164     162     151      84
                  55       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1600    1617 
   Size:       1600      17
 
EcoO109I rG'GnC_Cy

Cuts at:      0      94     108     166     175     225     424    1617 
   Size:         94      14      58       9      50     199    1193

  Fragments arranged by size: 

                1193     199      94      58      50      14       9
 
EcoRI G'AATT_C

Cuts at:      0    1169    1617 
   Size:       1169     448
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1485    1617 
   Size:       1485     132
 
HaeII r_GCGC'y

Cuts at:      0     974    1617 
   Size:        974     643
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     546     548     578     580    1617 
   Size:        546       2      30       2    1037

  Fragments arranged by size: 

                1037     546      30       2       2
 
HincII GTy'rAC

Cuts at:      0     270     311    1617 
   Size:        270      41    1306

  Fragments arranged by size: 

                1306     270      41
 
HindIII A'AGCT_T

Cuts at:      0    1214    1617 
   Size:       1214     403
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     182    1063    1098    1182    1592    1617 
   Size:        182     881      35      84     410      25

  Fragments arranged by size: 

                 881     410     182      84      35      25
 
MslI CAynn'nnrTG

Cuts at:      0     524     578    1110    1617 
   Size:        524      54     532     507

  Fragments arranged by size: 

                 532     524     507      54
 
MspA1I CmG'CkG

Cuts at:      0     747    1587    1617 
   Size:        747     840      30

  Fragments arranged by size: 

                 840     747      30
 
NspI r_CATG'y

Cuts at:      0    1575    1617 
   Size:       1575      42
 
PasI CC'CwG_GG

Cuts at:      0     221     345     428    1617 
   Size:        221     124      83    1189

  Fragments arranged by size: 

                1189     221     124      83
 
PciI A'CATG_T

Cuts at:      0    1571    1617 
   Size:       1571      46
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     283    1617 
   Size:        283    1334
 
PfoI T'CCnGG_A

Cuts at:      0     227    1598    1617 
   Size:        227    1371      19

  Fragments arranged by size: 

                1371     227      19
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     136     168    1617 
   Size:        136      32    1449

  Fragments arranged by size: 

                1449     136      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     166     225    1617 
   Size:        166      59    1392

  Fragments arranged by size: 

                1392     166      59
 
PspOMI G'GGCC_C

Cuts at:      0      93    1617 
   Size:         93    1524
 
PstI C_TGCA'G

Cuts at:      0     174    1617 
   Size:        174    1443
 
SbfI CC_TGCA'GG

Cuts at:      0     174    1617 
   Size:        174    1443
 
SfcI C'TryA_G

Cuts at:      0     114     170    1443    1617 
   Size:        114      56    1273     174

  Fragments arranged by size: 

                1273     174     114      56
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      92    1617 
   Size:         92    1525
 
SmaI CCC'GGG

Cuts at:      0      73    1617 
   Size:         73    1544
 
StuI AGG'CCT

Cuts at:      0    1384    1552    1617 
   Size:       1384     168      65

  Fragments arranged by size: 

                1384     168      65
 
TatI w'GTAC_w

Cuts at:      0     299     582    1617 
   Size:        299     283    1035

  Fragments arranged by size: 

                1035     299     283
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1307    1339    1381    1413    1617 
   Size:       1307      32      42      32     204

  Fragments arranged by size: 

                1307     204      42      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1079    1617 
   Size:       1079     538
 
XmaI C'CCGG_G

Cuts at:      0      71    1617 
   Size:         71    1546
 
XmnI GAAnn'nnTTC

Cuts at:      0     712     796     880    1048    1216    1384    1468    1552 
   Size:        712      84      84     168     168     168      84      84

Cuts at:   1552    1617 
   Size:         65

  Fragments arranged by size: 

                 712     168     168     168      84      84      84      84
                  65
 

 Enzymes that do cut:

     AcuI   AflIII     AgeI     AleI    AlwNI     ApaI     ApoI     AvaI
    BamHI     BanI    BanII     BbsI     BclI     BglI Bme1580I    BmgBI
     BmrI     BpmI     BsaI    BsaAI    BsaWI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    CspCI     EarI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII
  HindIII     MmeI     MslI   MspA1I     NspI     PasI     PciI    PflMI
     PfoI     PpiI    PpuMI   PspOMI     PstI     SbfI     SfcI     SfiI
     SmaI     StuI     TatI     TstI  Tth111I     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AhdI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
     BbeI    BbvCI     BcgI    BciVI    BfrBI    BglII     BlpI     BmtI
     BplI   Bpu10I    BpuEI    BsaBI    BsaHI    BsaXI    BseRI    BsiEI
    BsiWI    BsmBI    BspEI    BsrBI   BssHII    BstBI  BstZ17I    BtgZI
     BtsI     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoRV     FseI     FspI    FspAI     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PmeI     PmlI    PshAI
     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     ScaI    SexAI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
    TaqII     XbaI     XcmI     XhoI     ZraI
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