Restriction map for pF1KB8332
 (Linear) (Six-base) MAPSORT of: KIBB8332.seq  Check: 3381  from: 1  to: 1836



KIBB8332.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     694    1836 
   Size:        694    1142
 
AccI GT'mk_AC

Cuts at:      0     359     388    1394    1836 
   Size:        359      29    1006     442

  Fragments arranged by size: 

                1006     442     359      29
 
Acc65I G'GTAC_C

Cuts at:      0    1599    1836 
   Size:       1599     237
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     503     569    1712    1836 
   Size:        200     303      66    1143     124

  Fragments arranged by size: 

                1143     303     200     124      66
 
AlwNI CAG_nnn'CTG

Cuts at:      0      51    1054    1219    1836 
   Size:         51    1003     165     617

  Fragments arranged by size: 

                1003     617     165      51
 
ApoI r'AATT_y

Cuts at:      0     583     946    1139    1836 
   Size:        583     363     193     697

  Fragments arranged by size: 

                 697     583     363     193
 
AseI AT'TA_AT

Cuts at:      0    1784    1836 
   Size:       1784      52
 
AvaI C'yCGr_G

Cuts at:      0     371    1836 
   Size:        371    1465
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1583    1616    1836 
   Size:       1583      33     220

  Fragments arranged by size: 

                1583     220      33
 
BanI G'GyrC_C

Cuts at:      0     301    1599    1836 
   Size:        301    1298     237

  Fragments arranged by size: 

                1298     301     237
 
BanII G_rGCy'C

Cuts at:      0     630    1836 
   Size:        630    1206
 
BbeI G_GCGC'C

Cuts at:      0     305    1836 
   Size:        305    1531
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     686    1836 
   Size:        686    1150
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     803    1836 
   Size:        803    1033
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1307    1836 
   Size:       1307     529
 
BglII A'GATC_T

Cuts at:      0     153    1836 
   Size:        153    1683
 
Bme1580I G_kGCm'C

Cuts at:      0    1758    1836 
   Size:       1758      78
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     194     314     392    1010    1836 
   Size:        194     120      78     618     826

  Fragments arranged by size: 

                 826     618     194     120      78
 
BsaAI yAC'GTr

Cuts at:      0     386    1836 
   Size:        386    1450
 
BsaBI GATnn'nnATC

Cuts at:      0     646    1120    1836 
   Size:        646     474     716

  Fragments arranged by size: 

                 716     646     474
 
BsaHI Gr'CG_yC

Cuts at:      0     302    1836 
   Size:        302    1534
 
BsaWI w'CCGG_w

Cuts at:      0     795    1836 
   Size:        795    1041
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     390     420    1836 
   Size:        390      30    1416

  Fragments arranged by size: 

                1416     390      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     221     359    1822    1836 
   Size:        221     138    1463      14

  Fragments arranged by size: 

                1463     221     138      14
 
BseYI C'CCAG_C

Cuts at:      0    1530    1836 
   Size:       1530     306
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1040    1836 
   Size:       1040     796
 
BsiHKAI G_wGCw'C

Cuts at:      0     265     630    1836 
   Size:        265     365    1206

  Fragments arranged by size: 

                1206     365     265
 
Bsp1286I G_dGCh'C

Cuts at:      0     265     630    1758    1836 
   Size:        265     365    1128      78

  Fragments arranged by size: 

                1128     365     265      78
 
BspEI T'CCGG_A

Cuts at:      0     795    1836 
   Size:        795    1041
 
BspHI T'CATG_A

Cuts at:      0     537     840    1836 
   Size:        537     303     996

  Fragments arranged by size: 

                 996     537     303
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     694    1836 
   Size:        694    1142
 
BsrDI GCAATG_nn'

Cuts at:      0     184    1749    1836 
   Size:        184    1565      87

  Fragments arranged by size: 

                1565     184      87
 
BsrGI T'GTAC_A

Cuts at:      0    1289    1836 
   Size:       1289     547
 
BssSI C'ACGA_G

Cuts at:      0     805    1836 
   Size:        805    1031
 
BstEII G'GTnAC_C

Cuts at:      0    1517    1836 
   Size:       1517     319
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     292    1836 
   Size:        292    1544
 
BstYI r'GATC_y

Cuts at:      0     153     310    1836 
   Size:        153     157    1526

  Fragments arranged by size: 

                1526     157     153
 
BstZ17I GTA'TAC

Cuts at:      0     389    1836 
   Size:        389    1447
 
BtgI C'CryG_G

Cuts at:      0     305    1305    1836 
   Size:        305    1000     531

  Fragments arranged by size: 

                1000     531     305
 
BtsI GCAGTG_nn'

Cuts at:      0     909    1836 
   Size:        909     927
 
DraI TTT'AAA

Cuts at:      0     500    1836 
   Size:        500    1336
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1230    1836 
   Size:       1230     606
 
EaeI y'GGCC_r

Cuts at:      0    1764    1836 
   Size:       1764      72
 
EarI CTCTTCn'nnn_

Cuts at:      0      70     431     482    1836 
   Size:         70     361      51    1354

  Fragments arranged by size: 

                1354     361      70      51
 
EcoICRI GAG'CTC

Cuts at:      0     628    1836 
   Size:        628    1208
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     194     200     314     392     503     569    1010    1712 
   Size:        194       6     114      78     111      66     441     702

Cuts at:   1712    1836 
   Size:        124

  Fragments arranged by size: 

                 702     441     194     124     114     111      78      66
                   6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     322    1836 
   Size:        322    1514
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     205    1836 
   Size:        205    1631
 
HaeII r_GCGC'y

Cuts at:      0     305    1836 
   Size:        305    1531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     365     397     733     765     769     801    1836 
   Size:        365      32     336      32       4      32    1035

  Fragments arranged by size: 

                1035     365     336      32      32      32       4
 
HincII GTy'rAC

Cuts at:      0     360    1254    1836 
   Size:        360     894     582

  Fragments arranged by size: 

                 894     582     360
 
HindIII A'AGCT_T

Cuts at:      0    1441    1836 
   Size:       1441     395
 
KasI G'GCGC_C

Cuts at:      0     301    1836 
   Size:        301    1535
 
KpnI G_GTAC'C

Cuts at:      0    1603    1836 
   Size:       1603     233
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      11    1071    1224    1365    1836 
   Size:         11    1060     153     141     471

  Fragments arranged by size: 

                1060     471     153     141      11
 
MslI CAynn'nnrTG

Cuts at:      0    1373    1618    1836 
   Size:       1373     245     218

  Fragments arranged by size: 

                1373     245     218
 
MspA1I CmG'CkG

Cuts at:      0     922    1054    1381    1836 
   Size:        922     132     327     455

  Fragments arranged by size: 

                 922     455     327     132
 
NarI GG'CG_CC

Cuts at:      0     302    1836 
   Size:        302    1534
 
NcoI C'CATG_G

Cuts at:      0    1305    1836 
   Size:       1305     531
 
NspI r_CATG'y

Cuts at:      0    1164    1301    1836 
   Size:       1164     137     535

  Fragments arranged by size: 

                1164     535     137
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1360    1836 
   Size:       1360     476
 
PpuMI rG'GwC_Cy

Cuts at:      0     322    1836 
   Size:        322    1514
 
PvuII CAG'CTG

Cuts at:      0     922    1054    1381    1836 
   Size:        922     132     327     455

  Fragments arranged by size: 

                 922     455     327     132
 
SacI G_AGCT'C

Cuts at:      0     630    1836 
   Size:        630    1206
 
SalI G'TCGA_C

Cuts at:      0     358    1836 
   Size:        358    1478
 
SfcI C'TryA_G

Cuts at:      0     660    1836 
   Size:        660    1176
 
SfoI GGC'GCC

Cuts at:      0     303    1836 
   Size:        303    1533
 
SmlI C'TyrA_G

Cuts at:      0     371    1836 
   Size:        371    1465
 
SphI G_CATG'C

Cuts at:      0    1301    1836 
   Size:       1301     535
 
StyI C'CwwG_G

Cuts at:      0    1305    1836 
   Size:       1305     531
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1544    1672    1836 
   Size:       1544     128     164

  Fragments arranged by size: 

                1544     164     128
 
TatI w'GTAC_w

Cuts at:      0    1289    1836 
   Size:       1289     547
 
XbaI T'CTAG_A

Cuts at:      0     313    1836 
   Size:        313    1523
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1617    1836 
   Size:       1617     219
 
XhoI C'TCGA_G

Cuts at:      0     371    1836 
   Size:        371    1465
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI    AlwNI     ApoI     AseI     AvaI
     BaeI     BanI    BanII     BbeI     BbsI    BciVI     BglI    BglII
 Bme1580I     BpmI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrDI
    BsrGI    BssSI   BstEII    BstXI    BstYI  BstZ17I     BtgI     BtsI
     DraI     DrdI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I     FalI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MmeI     MslI
   MspA1I     NarI     NcoI     NspI    PflMI    PpuMI    PvuII     SacI
     SalI     SfcI     SfoI     SmlI     SphI     StyI    TaqII     TatI
     XbaI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII    BamHI
    BbvCI     BcgI     BclI    BfrBI     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsiEI    BsiWI     BsmI    BsmBI
    BsrBI    BsrFI   BssHII   BstAPI    BstBI   Bsu36I    BtgZI     ClaI
    CspCI   DraIII     EagI     EciI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI     HpaI     MfeI     MluI     MscI     NaeI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII    SacII    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XmaI     XmnI
     ZraI
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