(Circular) (Six-base) MAPSORT of: pF1KB8326.seq Check: 1188 from: 1 to: 4211 pF1KB8326 4211 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 351 695 351 Size: 344 3867 AccI GT'mk_AC Cuts at: 1249 1249 Size: 4211 Acc65I G'GTAC_C Cuts at: 1227 1227 Size: 4211 AclI AA'CG_TT Cuts at: 4051 4051 Size: 4211 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2036 2468 3215 3483 2036 Size: 432 747 268 2764 Fragments arranged by size: 2764 747 432 268 AflIII A'CryG_T Cuts at: 1563 2667 3644 1563 Size: 1104 977 2130 Fragments arranged by size: 2130 1104 977 AgeI A'CCGG_T Cuts at: 2599 2599 Size: 4211 AlwNI CAG_nnn'CTG Cuts at: 126 279 459 1516 3083 126 Size: 153 180 1057 1567 1254 Fragments arranged by size: 1567 1254 1057 180 153 ApaI G_GGCC'C Cuts at: 777 777 Size: 4211 ApaLI G'TGCA_C Cuts at: 682 2981 682 Size: 2299 1912 ApoI r'AATT_y Cuts at: 1215 3564 3820 1215 Size: 2349 256 1606 Fragments arranged by size: 2349 1606 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4211 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4211 AvaI C'yCGr_G Cuts at: 673 1231 673 Size: 558 3653 BamHI G'GATC_C Cuts at: 1236 1236 Size: 4211 BanI G'GyrC_C Cuts at: 436 598 696 1020 1227 1641 1889 1924 436 Size: 162 98 324 207 414 248 35 2723 Fragments arranged by size: 2723 414 324 248 207 162 98 35 BanII G_rGCy'C Cuts at: 777 1013 1088 1199 1225 2255 777 Size: 236 75 111 26 1030 2733 Fragments arranged by size: 2733 1030 236 111 75 26 BbeI G_GCGC'C Cuts at: 1645 1893 1645 Size: 248 3963 BbsI GAAGACnn'nnnn_ Cuts at: 725 725 Size: 4211 BbvCI CC'TCA_GC Cuts at: 574 574 Size: 4211 BciVI GTATCCnnnnn_n' Cuts at: 2109 2870 4211 2109 Size: 761 1341 2109 Fragments arranged by size: 2109 1341 761 BclI T'GATC_A Cuts at: 1731 1731 Size: 4211 BfrBI ATG'CAT Cuts at: 405 405 Size: 4211 BglI GCCn_nnn'nGGC Cuts at: 289 789 289 Size: 500 3711 BglII A'GATC_T Cuts at: 1726 3763 1726 Size: 2037 2174 BlpI GC'TnA_GC Cuts at: 1323 1323 Size: 4211 Bme1580I G_kGCm'C Cuts at: 183 320 686 701 777 1105 1836 1929 2985 Size: 137 366 15 76 328 731 93 1056 Cuts at: 2985 183 Size: 1409 Fragments arranged by size: 1409 1056 731 366 328 137 93 76 15 BmrI ACTGGGnnnn_n' Cuts at: 1423 1504 1839 3983 1423 Size: 81 335 2144 1651 Fragments arranged by size: 2144 1651 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2578 2578 Size: 4211 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 268 788 904 268 Size: 520 116 3575 Fragments arranged by size: 3575 520 116 Bpu10I CC'TnA_GC Cuts at: 441 574 441 Size: 133 4078 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1387 2266 2758 3056 1387 Size: 879 492 298 2542 Fragments arranged by size: 2542 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 247 516 651 3915 247 Size: 269 135 3264 543 Fragments arranged by size: 3264 543 269 135 BsaAI yAC'GTr Cuts at: 1564 1564 Size: 4211 BsaBI GATnn'nnATC Cuts at: 6 1725 3762 6 Size: 1719 2037 455 Fragments arranged by size: 2037 1719 455 BsaHI Gr'CG_yC Cuts at: 1151 1642 1890 2592 4088 1151 Size: 491 248 702 1496 1274 Fragments arranged by size: 1496 1274 702 491 248 BsaWI w'CCGG_w Cuts at: 906 1400 1921 2599 2873 3020 3431 906 Size: 494 521 678 274 147 411 1686 Fragments arranged by size: 1686 678 521 494 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 877 907 877 Size: 30 4181 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 265 714 830 901 932 935 265 Size: 449 116 71 31 3 3541 Fragments arranged by size: 3541 449 116 71 31 3 BseYI C'CCAG_C Cuts at: 384 820 1012 1016 1198 1636 2394 2971 3467 Size: 436 192 4 182 438 758 577 496 Cuts at: 3467 384 Size: 1128 Fragments arranged by size: 1128 758 577 496 438 436 192 182 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 241 241 Size: 4211 BsiEI CG_ry'CG Cuts at: 104 749 1753 1799 3007 104 Size: 645 1004 46 1208 1308 Fragments arranged by size: 1308 1208 1004 645 46 BsiHKAI G_wGCw'C Cuts at: 686 1088 1225 2003 2193 2985 686 Size: 402 137 778 190 792 1912 Fragments arranged by size: 1912 792 778 402 190 137 Bsp1286I G_dGCh'C Cuts at: 183 320 686 701 777 1013 1088 1105 1199 Size: 137 366 15 76 236 75 17 94 Cuts at: 1199 1225 1836 1929 2003 2193 2255 2985 183 Size: 26 611 93 74 190 62 730 1409 Fragments arranged by size: 1409 730 611 366 236 190 137 94 93 76 75 74 62 26 17 15 BspEI T'CCGG_A Cuts at: 3431 3431 Size: 4211 BspHI T'CATG_A Cuts at: 3387 3387 Size: 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 347 351 695 944 1261 1475 1777 2158 347 Size: 4 344 249 317 214 302 381 2400 Fragments arranged by size: 2400 381 344 317 302 249 214 4 BsrBI CCG'CTC Cuts at: 50 2503 2557 4041 4206 50 Size: 2453 54 1484 165 55 Fragments arranged by size: 2453 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2123 2123 Size: 4211 BsrFI r'CCGG_y Cuts at: 690 748 2209 2390 2599 690 Size: 58 1461 181 209 2302 Fragments arranged by size: 2302 1461 209 181 58 BssSI C'ACGA_G Cuts at: 2482 2840 2482 Size: 358 3853 BstAPI GCAn_nnn'nTGC Cuts at: 324 1588 324 Size: 1264 2947 BstBI TT'CG_AA Cuts at: 2572 2572 Size: 4211 BstXI CCAn_nnnn'nTGG Cuts at: 828 828 Size: 4211 BstYI r'GATC_y Cuts at: 1 140 1236 1703 1726 2061 2307 3319 3405 Size: 139 1096 467 23 335 246 1012 86 Cuts at: 3405 3417 3763 1 Size: 12 346 449 Fragments arranged by size: 1096 1012 467 449 346 335 246 139 86 23 12 BtgI C'CryG_G Cuts at: 107 257 558 107 Size: 150 301 3760 Fragments arranged by size: 3760 301 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 465 2161 2342 2505 465 Size: 1696 181 163 2171 Fragments arranged by size: 2171 1696 181 163 BtsI GCAGTG_nn' Cuts at: 1454 1454 Size: 4211 DraI TTT'AAA Cuts at: 1210 1210 Size: 4211 DrdI GACnn_nn'nnGTC Cuts at: 1917 2775 1917 Size: 858 3353 EaeI y'GGCC_r Cuts at: 150 281 311 468 555 746 1796 1970 2361 Size: 131 30 157 87 191 1050 174 391 Cuts at: 2361 2388 3733 150 Size: 27 1345 628 Fragments arranged by size: 1345 1050 628 391 191 174 157 131 87 30 27 EagI C'GGCC_G Cuts at: 746 1796 746 Size: 1050 3161 EarI CTCTTCn'nnn_ Cuts at: 908 2234 2444 908 Size: 1326 210 2675 Fragments arranged by size: 2675 1326 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 127 633 705 2419 2729 2875 4021 127 Size: 506 72 1714 310 146 1146 317 Fragments arranged by size: 1714 1146 506 317 310 146 72 EcoICRI GAG'CTC Cuts at: 1086 1223 1086 Size: 137 4074 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 268 788 904 2036 2468 3215 3483 268 Size: 520 116 1132 432 747 268 996 Fragments arranged by size: 1132 996 747 520 432 268 116 EcoNI CCTnn'n_nnAGG Cuts at: 358 516 358 Size: 158 4053 EcoO109I rG'GnC_Cy Cuts at: 570 624 773 774 1350 570 Size: 54 149 1 576 3431 Fragments arranged by size: 3431 576 149 54 1 EcoRI G'AATT_C Cuts at: 1215 1215 Size: 4211 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2230 2230 Size: 4211 FspI TGC'GCA Cuts at: 328 1992 328 Size: 1664 2547 HaeII r_GCGC'y Cuts at: 247 758 1645 1893 2915 3894 247 Size: 511 887 248 1022 979 564 Fragments arranged by size: 1022 979 887 564 511 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 877 909 2053 2083 2085 2115 877 Size: 32 1144 30 2 30 2973 Fragments arranged by size: 2973 1144 32 30 30 2 HincII GTy'rAC Cuts at: 1250 1250 Size: 4211 KasI G'GCGC_C Cuts at: 1641 1889 1641 Size: 248 3963 KpnI G_GTAC'C Cuts at: 1231 1231 Size: 4211 MluI A'CGCG_T Cuts at: 3644 3644 Size: 4211 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 314 2882 3066 314 Size: 2568 184 1459 Fragments arranged by size: 2568 1459 184 MscI TGG'CCA Cuts at: 283 557 1972 283 Size: 274 1415 2522 Fragments arranged by size: 2522 1415 274 MslI CAynn'nnrTG Cuts at: 406 2200 2327 406 Size: 1794 127 2290 Fragments arranged by size: 2290 1794 127 MspA1I CmG'CkG Cuts at: 126 456 585 870 1016 1202 1322 1636 1996 Size: 330 129 285 146 186 120 314 360 Cuts at: 1996 3009 3254 126 Size: 1013 245 1083 Fragments arranged by size: 1083 1013 360 330 314 285 245 186 146 129 120 NaeI GCC'GGC Cuts at: 750 2392 750 Size: 1642 2569 NarI GG'CG_CC Cuts at: 1642 1890 1642 Size: 248 3963 NcoI C'CATG_G Cuts at: 107 257 558 107 Size: 150 301 3760 Fragments arranged by size: 3760 301 150 NdeI CA'TA_TG Cuts at: 407 407 Size: 4211 NgoMIV G'CCGG_C Cuts at: 748 2390 748 Size: 1642 2569 NsiI A_TGCA'T Cuts at: 407 407 Size: 4211 NspI r_CATG'y Cuts at: 333 405 1264 1764 2671 333 Size: 72 859 500 907 1873 Fragments arranged by size: 1873 907 859 500 72 PasI CC'CwG_GG Cuts at: 1180 1180 Size: 4211 PciI A'CATG_T Cuts at: 2667 2667 Size: 4211 PflMI CCAn_nnn'nTGG Cuts at: 279 997 279 Size: 718 3493 PmeI GTTT'AAAC Cuts at: 1210 1210 Size: 4211 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3350 3382 3350 Size: 32 4179 PpuMI rG'GwC_Cy Cuts at: 570 570 Size: 4211 PspOMI G'GGCC_C Cuts at: 773 773 Size: 4211 PstI C_TGCA'G Cuts at: 131 344 520 1258 1943 131 Size: 213 176 738 685 2399 Fragments arranged by size: 2399 738 685 213 176 PvuI CG_AT'CG Cuts at: 104 104 Size: 4211 PvuII CAG'CTG Cuts at: 126 585 1016 1202 1636 1996 126 Size: 459 431 186 434 360 2341 Fragments arranged by size: 2341 459 434 431 360 186 RsrII CG'GwC_CG Cuts at: 2406 2406 Size: 4211 SacI G_AGCT'C Cuts at: 1088 1225 1088 Size: 137 4074 SalI G'TCGA_C Cuts at: 1248 1248 Size: 4211 SapI GCTCTTCn'nnn_ Cuts at: 908 2234 2444 908 Size: 1326 210 2675 Fragments arranged by size: 2675 1326 210 SbfI CC_TGCA'GG Cuts at: 344 1258 344 Size: 914 3297 ScaI AGT'ACT Cuts at: 3728 3728 Size: 4211 SexAI A'CCwGG_T Cuts at: 296 296 Size: 4211 SfcI C'TryA_G Cuts at: 33 118 127 340 516 1254 1939 2932 3123 Size: 85 9 213 176 738 685 993 191 Cuts at: 3123 33 Size: 1121 Fragments arranged by size: 1121 993 738 685 213 191 176 85 9 SfiI GGCCn_nnn'nGGCC Cuts at: 289 289 Size: 4211 SfoI GGC'GCC Cuts at: 1643 1891 1643 Size: 248 3963 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4211 SmaI CCC'GGG Cuts at: 1233 1233 Size: 4211 SmlI C'TyrA_G Cuts at: 1366 2281 2773 3035 1366 Size: 915 492 262 2542 Fragments arranged by size: 2542 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4211 SphI G_CATG'C Cuts at: 1264 1764 1264 Size: 500 3711 StyI C'CwwG_G Cuts at: 107 257 558 650 785 827 1345 1699 107 Size: 150 301 92 135 42 518 354 2619 Fragments arranged by size: 2619 518 354 301 150 135 92 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1008 1794 1927 2386 2595 1008 Size: 786 133 459 209 2624 Fragments arranged by size: 2624 786 459 209 133 TatI w'GTAC_w Cuts at: 3726 3726 Size: 4211 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3728 3760 3728 Size: 32 4179 Tth111I GACn'n_nGTC Cuts at: 2008 2008 Size: 4211 XbaI T'CTAG_A Cuts at: 1242 1242 Size: 4211 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1706 1706 Size: 4211 XmaI C'CCGG_G Cuts at: 1231 1231 Size: 4211 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BcgI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI NruI PacI PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI