(Linear) (Six-base) MAPSORT of: KIBB8322.seq Check: 8781 from: 1 to: 2004 KIBB8322.orf With 182 enzymes: SgfI * March 3, 2008 11:05 .. AccI GT'mk_AC Cuts at: 0 1423 2004 Size: 1423 581 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 21 1341 2004 Size: 21 1320 663 Fragments arranged by size: 1320 663 21 AfeI AGC'GCT Cuts at: 0 556 661 679 1705 2004 Size: 556 105 18 1026 299 Fragments arranged by size: 1026 556 299 105 18 AflIII A'CryG_T Cuts at: 0 524 1169 1568 1818 2004 Size: 524 645 399 250 186 Fragments arranged by size: 645 524 399 250 186 AhdI GACnn_n'nnGTC Cuts at: 0 462 2004 Size: 462 1542 AleI CACnn'nnGTG Cuts at: 0 89 1583 1669 2004 Size: 89 1494 86 335 Fragments arranged by size: 1494 335 89 86 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 64 153 2004 Size: 64 89 1851 Fragments arranged by size: 1851 89 64 AlwNI CAG_nnn'CTG Cuts at: 0 822 1374 1458 1610 2004 Size: 822 552 84 152 394 Fragments arranged by size: 822 552 394 152 84 ApaLI G'TGCA_C Cuts at: 0 345 846 1518 1732 2004 Size: 345 501 672 214 272 Fragments arranged by size: 672 501 345 272 214 AvaI C'yCGr_G Cuts at: 0 52 384 399 427 697 909 1006 2004 Size: 52 332 15 28 270 212 97 998 Fragments arranged by size: 998 332 270 212 97 52 28 15 BamHI G'GATC_C Cuts at: 0 389 2004 Size: 389 1615 BanI G'GyrC_C Cuts at: 0 874 931 1873 2004 Size: 874 57 942 131 Fragments arranged by size: 942 874 131 57 BanII G_rGCy'C Cuts at: 0 890 1723 2004 Size: 890 833 281 Fragments arranged by size: 890 833 281 BbsI GAAGACnn'nnnn_ Cuts at: 0 635 860 2004 Size: 635 225 1144 Fragments arranged by size: 1144 635 225 BbvCI CC'TCA_GC Cuts at: 0 1031 2004 Size: 1031 973 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 336 370 997 1031 2004 Size: 336 34 627 34 973 Fragments arranged by size: 973 627 336 34 34 BfrBI ATG'CAT Cuts at: 0 1989 2004 Size: 1989 15 BglI GCCn_nnn'nGGC Cuts at: 0 1892 2004 Size: 1892 112 Bme1580I G_kGCm'C Cuts at: 0 349 424 850 877 1522 1736 1878 2004 Size: 349 75 426 27 645 214 142 126 Fragments arranged by size: 645 426 349 214 142 126 75 27 BmgBI CAC'GTC Cuts at: 0 440 1172 2004 Size: 440 732 832 Fragments arranged by size: 832 732 440 BmrI ACTGGGnnnn_n' Cuts at: 0 1386 2004 Size: 1386 618 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 960 1265 2004 Size: 960 305 739 Fragments arranged by size: 960 739 305 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1831 2004 Size: 1831 173 Bpu10I CC'TnA_GC Cuts at: 0 1031 2004 Size: 1031 973 BsaI GGTCTCn'nnnn_ Cuts at: 0 49 279 694 2004 Size: 49 230 415 1310 Fragments arranged by size: 1310 415 230 49 BsaAI yAC'GTr Cuts at: 0 1821 2004 Size: 1821 183 BsaHI Gr'CG_yC Cuts at: 0 914 2004 Size: 914 1090 BsaWI w'CCGG_w Cuts at: 0 1077 2004 Size: 1077 927 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 514 544 943 973 1915 1945 2004 Size: 514 30 399 30 942 30 59 Fragments arranged by size: 942 514 399 59 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 856 912 2004 Size: 856 56 1092 Fragments arranged by size: 1092 856 56 BseYI C'CCAG_C Cuts at: 0 634 1199 1322 1409 1941 2004 Size: 634 565 123 87 532 63 Fragments arranged by size: 634 565 532 123 87 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 835 1418 1813 1890 2004 Size: 244 591 583 395 77 114 Fragments arranged by size: 591 583 395 244 114 77 BsiEI CG_ry'CG Cuts at: 0 308 2004 Size: 308 1696 BsiHKAI G_wGCw'C Cuts at: 0 53 349 532 850 1522 1723 1736 2004 Size: 53 296 183 318 672 201 13 268 Fragments arranged by size: 672 318 296 268 201 183 53 13 BsmI GAATG_Cn' Cuts at: 0 11 2004 Size: 11 1993 Bsp1286I G_dGCh'C Cuts at: 0 53 349 424 532 850 877 890 1522 Size: 53 296 75 108 318 27 13 632 Cuts at: 1522 1723 1736 1878 2004 Size: 201 13 142 126 Fragments arranged by size: 632 318 296 201 142 126 108 75 53 27 13 13 BspEI T'CCGG_A Cuts at: 0 1077 2004 Size: 1077 927 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 482 1263 1310 2004 Size: 482 781 47 694 Fragments arranged by size: 781 694 482 47 BsrDI GCAATG_nn' Cuts at: 0 1095 1186 1848 2004 Size: 1095 91 662 156 Fragments arranged by size: 1095 662 156 91 BsrFI r'CCGG_y Cuts at: 0 151 2004 Size: 151 1853 BsrGI T'GTAC_A Cuts at: 0 254 2004 Size: 254 1750 BssHII G'CGCG_C Cuts at: 0 126 2004 Size: 126 1878 BssSI C'ACGA_G Cuts at: 0 589 2004 Size: 589 1415 BstAPI GCAn_nnn'nTGC Cuts at: 0 231 813 1188 1526 2004 Size: 231 582 375 338 478 Fragments arranged by size: 582 478 375 338 231 BstYI r'GATC_y Cuts at: 0 203 296 389 506 818 2004 Size: 203 93 93 117 312 1186 Fragments arranged by size: 1186 312 203 117 93 93 Bsu36I CC'TnA_GG Cuts at: 0 1240 2004 Size: 1240 764 BtgI C'CryG_G Cuts at: 0 78 111 415 1328 2004 Size: 78 33 304 913 676 Fragments arranged by size: 913 676 304 78 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 389 2004 Size: 389 1615 BtsI GCAGTG_nn' Cuts at: 0 581 708 1341 2004 Size: 581 127 633 663 Fragments arranged by size: 663 633 581 127 DraIII CAC_nnn'GTG Cuts at: 0 498 1656 2004 Size: 498 1158 348 Fragments arranged by size: 1158 498 348 DrdI GACnn_nn'nnGTC Cuts at: 0 913 1144 2004 Size: 913 231 860 Fragments arranged by size: 913 860 231 EaeI y'GGCC_r Cuts at: 0 81 305 2004 Size: 81 224 1699 Fragments arranged by size: 1699 224 81 EagI C'GGCC_G Cuts at: 0 305 2004 Size: 305 1699 EarI CTCTTCn'nnn_ Cuts at: 0 1318 1360 1711 2004 Size: 1318 42 351 293 Fragments arranged by size: 1318 351 293 42 EcoICRI GAG'CTC Cuts at: 0 1721 2004 Size: 1721 283 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 21 1341 1831 2004 Size: 21 1320 490 173 Fragments arranged by size: 1320 490 173 21 EcoO109I rG'GnC_Cy Cuts at: 0 1847 2004 Size: 1847 157 FspI TGC'GCA Cuts at: 0 106 736 2004 Size: 106 630 1268 Fragments arranged by size: 1268 630 106 FspAI rTGC'GCAy Cuts at: 0 106 736 2004 Size: 106 630 1268 Fragments arranged by size: 1268 630 106 HaeII r_GCGC'y Cuts at: 0 558 663 681 1450 1620 1707 2004 Size: 558 105 18 769 170 87 297 Fragments arranged by size: 769 558 297 170 105 87 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 193 225 448 480 2004 Size: 193 32 223 32 1524 Fragments arranged by size: 1524 223 193 32 32 HincII GTy'rAC Cuts at: 0 1167 1424 2004 Size: 1167 257 580 Fragments arranged by size: 1167 580 257 HindIII A'AGCT_T Cuts at: 0 900 2004 Size: 900 1104 MluI A'CGCG_T Cuts at: 0 524 2004 Size: 524 1480 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 263 1160 2004 Size: 263 897 844 Fragments arranged by size: 897 844 263 MscI TGG'CCA Cuts at: 0 83 2004 Size: 83 1921 MslI CAynn'nnrTG Cuts at: 0 89 1583 1669 1683 1984 2004 Size: 89 1494 86 14 301 20 Fragments arranged by size: 1494 301 89 86 20 14 MspA1I CmG'CkG Cuts at: 0 137 260 417 548 569 638 1030 1288 Size: 137 123 157 131 21 69 392 258 Cuts at: 1288 1330 1440 2004 Size: 42 110 564 Fragments arranged by size: 564 392 258 157 137 131 123 110 69 42 21 NaeI GCC'GGC Cuts at: 0 153 2004 Size: 153 1851 NgoMIV G'CCGG_C Cuts at: 0 151 2004 Size: 151 1853 NsiI A_TGCA'T Cuts at: 0 1991 2004 Size: 1991 13 NspI r_CATG'y Cuts at: 0 123 1572 1740 1993 2004 Size: 123 1449 168 253 11 Fragments arranged by size: 1449 253 168 123 11 PciI A'CATG_T Cuts at: 0 1568 2004 Size: 1568 436 PflMI CCAn_nnn'nTGG Cuts at: 0 624 2004 Size: 624 1380 PfoI T'CCnGG_A Cuts at: 0 167 392 821 1808 2004 Size: 167 225 429 987 196 Fragments arranged by size: 987 429 225 196 167 PmlI CAC'GTG Cuts at: 0 1821 2004 Size: 1821 183 PpuMI rG'GwC_Cy Cuts at: 0 1847 2004 Size: 1847 157 PstI C_TGCA'G Cuts at: 0 296 323 479 1020 1860 2004 Size: 296 27 156 541 840 144 Fragments arranged by size: 840 541 296 156 144 27 PvuII CAG'CTG Cuts at: 0 638 1288 1440 2004 Size: 638 650 152 564 Fragments arranged by size: 650 638 564 152 SacI G_AGCT'C Cuts at: 0 1723 2004 Size: 1723 281 SacII CC_GC'GG Cuts at: 0 418 1331 2004 Size: 418 913 673 Fragments arranged by size: 913 673 418 SalI G'TCGA_C Cuts at: 0 1422 2004 Size: 1422 582 SanDI GG'GwC_CC Cuts at: 0 1847 2004 Size: 1847 157 SapI GCTCTTCn'nnn_ Cuts at: 0 1711 2004 Size: 1711 293 SbfI CC_TGCA'GG Cuts at: 0 1860 2004 Size: 1860 144 SfcI C'TryA_G Cuts at: 0 189 292 319 475 1016 1856 2004 Size: 189 103 27 156 541 840 148 Fragments arranged by size: 840 541 189 156 148 103 27 SmaI CCC'GGG Cuts at: 0 386 2004 Size: 386 1618 SmlI C'TyrA_G Cuts at: 0 52 2004 Size: 52 1952 SphI G_CATG'C Cuts at: 0 123 2004 Size: 123 1881 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 19 500 1015 1152 1726 2004 Size: 19 481 515 137 574 278 Fragments arranged by size: 574 515 481 278 137 19 TatI w'GTAC_w Cuts at: 0 254 2004 Size: 254 1750 Tth111I GACn'n_nGTC Cuts at: 0 1404 2004 Size: 1404 600 XhoI C'TCGA_G Cuts at: 0 52 2004 Size: 52 1952 XmaI C'CCGG_G Cuts at: 0 384 2004 Size: 384 1620 Enzymes that do cut: AccI AcuI AfeI AflIII AhdI AleI AlfI AlwNI ApaLI AvaI BamHI BanI BanII BbsI BbvCI BcgI BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I FspI FspAI HaeII Hin4I HincII HindIII MluI MmeI MscI MslI MspA1I NaeI NgoMIV NsiI NspI PciI PflMI PfoI PmlI PpuMI PstI PvuII SacI SacII SalI SanDI SapI SbfI SfcI SmaI SmlI SphI TaqII TatI Tth111I XhoI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI BglII BlpI BmtI BpuEI BsaBI BsiWI BsmBI BspHI BsrBI BstBI BstEII BstXI BstZ17I ClaI CspCI DraI EciI EcoNI EcoRI EcoRV FalI FseI HpaI KasI KpnI MfeI NarI NcoI NdeI NheI NotI NruI PacI PasI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI StyI SwaI TstI XbaI XcmI XmnI ZraI