################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BcnI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MaeII MluI MluNI MroI MscI MspI MvnI MwoI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TthHB8I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AflII 613 CTTAAG C^TTAAG AflIII 317 ACATGT A^C[GA][CT]GT AlwI 78 GATCC GGATC(4/5) AlwNI 187 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 91 GTGCTC G[AT]GC[AT]^C AspI 445 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 623 GTCTTC GAAGAC(2/6) BciVI 261 GTATCC GTATCC(6/5) BclI 639 TGATCA T^GATCA BfaI 58 CTAG C^TAG BfrI 613 CTTAAG C^TTAAG BglII 339 AGATCT A^GATCT BmyI 91 GTGCTC G[AGT]GC[ACT]^C BpuAI 623 GTCTTC GAAGAC(2/6) BsaBI 840 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 400 CTGCAC GTGCAG(16/14) BsiHKAI 91 GTGCTC G[AT]GC[AT]^C BsiYI 306 CCTTACAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 306 CCTTACAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 91 GTGCTC G[AGT]GC[ACT]^C BspLU11I 317 ACATGT A^CATGT BsrDI 200 GCAATG GCAATG(2/0) BsrI 50 CCAGT ACTGG(1/-1) DraI 213 TTTAAA TTT^AAA DsaI 113 CCATGG C^C[GA][CT]GG EcoNI 306 CCTTACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 538 GAATTC G^AATTC EcoT14I 113 CCATGG C^C[AT][AT]GG FbaI 639 TGATCA T^GATCA HincII 205 GTTGAC GT[CT]^[GA]AC HindII 205 GTTGAC GT[CT]^[GA]AC HphI 154 GGTGA GGTGA(8/7) MaeI 58 CTAG C^TAG MamI 840 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 149 CAATTG C^AATTG MslI 789 CACAGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 226 CAGCTG C[AC]G^C[GT]G MunI 149 CAATTG C^AATTG NcoI 113 CCATGG C^CATGG PflFI 445 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 5 CTGCAG CTGCA^G PvuII 226 CAGCTG CAG^CTG SapI 597 GAAGAGC GCTCTTC(1/4) ScaI 761 AGTACT AGT^ACT SmlI 613 CTTAAG C^T[CT][GA]AG StyI 113 CCATGG C^C[AT][AT]GG Tsp45I 443 GTGAC ^GT[GC]AC Tth111I 445 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 57 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 195 GCGG CCGC(-3/-1) 771 GCGG AfaI 321 GTAC GT^AC 762 GTAC Asp700I 538 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 555 GAACCTTTTC BstYI 77 AGATCC [GA]^GATC[CT] 339 AGATCT Cac8I 3 GCCTGC GC[ACGT]^[ACGT]GC 679 GCAGGC MaeIII 98 GTTAC ^GT[ACGT]AC 443 GTGAC MflI 77 AGATCC [GA]^GATC[CT] 339 AGATCT NspI 317 ACATGT [GA]CATG^[CT] 866 ACATGC PleI 688 GACTC GAGTC(4/5) 823 GACTC RsaI 321 GTAC GT^AC 762 GTAC SfaNI 754 GATGC GCATC(5/9) 845 GCATC SfcI 5 CTGCAG C^T[GA][CT]AG 361 CTACAG TspRI 398 CACTG CA[GC]TG[ACGT][ACGT]^ 692 CACTG XhoII 77 AGATCC [GA]^GATC[CT] 339 AGATCT XmnI 538 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 555 GAACCTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AcsI AluI ApoI BbvI BsaJI BssKI BstF5I BstNI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MboI MboII MnlI MseI MvaI NdeII NlaIII Sau3AI Sau96I ScrFI TfiI Tru9I TseI Tsp509I