Restriction map for pF1KB8319
 (Linear) (Six-base) MAPSORT of: KIBB8319.seq  Check: 6870  from: 1  to: 897



KIBB8319.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     497     897 
   Size:        265     232     400

  Fragments arranged by size: 

                 400     265     232
 
AflII C'TTAA_G

Cuts at:      0     481     897 
   Size:        481     416
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     196     897 
   Size:        196     701
 
BamHI G'GATC_C

Cuts at:      0     215     618     897 
   Size:        215     403     279

  Fragments arranged by size: 

                 403     279     215
 
BanII G_rGCy'C

Cuts at:      0     362     763     897 
   Size:        362     401     134

  Fragments arranged by size: 

                 401     362     134
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     295     497     897 
   Size:        295     202     400

  Fragments arranged by size: 

                 400     295     202
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      55     897 
   Size:         55     842
 
BsaBI GATnn'nnATC

Cuts at:      0     881     897 
   Size:        881      16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     690     807     897 
   Size:        166     524     117      90

  Fragments arranged by size: 

                 524     166     117      90
 
BsiHKAI G_wGCw'C

Cuts at:      0     763     897 
   Size:        763     134
 
Bsp1286I G_dGCh'C

Cuts at:      0     362     763     897 
   Size:        362     401     134

  Fragments arranged by size: 

                 401     362     134
 
BspHI T'CATG_A

Cuts at:      0     405     897 
   Size:        405     492
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     428     531     897 
   Size:        428     103     366

  Fragments arranged by size: 

                 428     366     103
 
BsrDI GCAATG_nn'

Cuts at:      0     117     160     897 
   Size:        117      43     737

  Fragments arranged by size: 

                 737     117      43
 
BsrFI r'CCGG_y

Cuts at:      0     106     897 
   Size:        106     791
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     150     897 
   Size:        150     747
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     573     897 
   Size:        573     324
 
BstYI r'GATC_y

Cuts at:      0      14     215     591     618     642     897 
   Size:         14     201     376      27      24     255

  Fragments arranged by size: 

                 376     255     201      27      24      14
 
Bsu36I CC'TnA_GG

Cuts at:      0     371     897 
   Size:        371     526
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     815     897 
   Size:        815      82
 
BtsI GCAGTG_nn'

Cuts at:      0      44     897 
   Size:         44     853
 
EaeI y'GGCC_r

Cuts at:      0      24     897 
   Size:         24     873
 
EcoICRI GAG'CTC

Cuts at:      0     761     897 
   Size:        761     136
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     497     897 
   Size:        265     232     400

  Fragments arranged by size: 

                 400     265     232
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      40      72     897 
   Size:         40      32     825

  Fragments arranged by size: 

                 825      40      32
 
HincII GTy'rAC

Cuts at:      0     104     897 
   Size:        104     793
 
HindIII A'AGCT_T

Cuts at:      0      91     484     897 
   Size:         91     393     413

  Fragments arranged by size: 

                 413     393      91
 
HpaI GTT'AAC

Cuts at:      0     104     897 
   Size:        104     793
 
MscI TGG'CCA

Cuts at:      0      26     897 
   Size:         26     871
 
MslI CAynn'nnrTG

Cuts at:      0     410     897 
   Size:        410     487
 
MspA1I CmG'CkG

Cuts at:      0     147     265     354     812     821     897 
   Size:        147     118      89     458       9      76

  Fragments arranged by size: 

                 458     147     118      89      76       9
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      33     551     897 
   Size:         33     518     346

  Fragments arranged by size: 

                 518     346      33
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     371     403     897 
   Size:        371      32     494

  Fragments arranged by size: 

                 494     371      32
 
PstI C_TGCA'G

Cuts at:      0     353     897 
   Size:        353     544
 
PvuII CAG'CTG

Cuts at:      0     147     265     821     897 
   Size:        147     118     556      76

  Fragments arranged by size: 

                 556     147     118      76
 
SacI G_AGCT'C

Cuts at:      0     763     897 
   Size:        763     134
 
SfcI C'TryA_G

Cuts at:      0     266     349     897 
   Size:        266      83     548

  Fragments arranged by size: 

                 548     266      83
 
SmlI C'TyrA_G

Cuts at:      0     481     897 
   Size:        481     416
 
StuI AGG'CCT

Cuts at:      0     694     897 
   Size:        694     203
 
StyI C'CwwG_G

Cuts at:      0     399     831     897 
   Size:        399     432      66

  Fragments arranged by size: 

                 432     399      66
 
TatI w'GTAC_w

Cuts at:      0     378     897 
   Size:        378     519
 

 Enzymes that do cut:

     AcuI    AflII     AlfI    BamHI    BanII     BbsI     BmrI    BsaBI
     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI   BstAPI
    BstXI    BstYI   Bsu36I    BtgZI     BtsI     EaeI  EcoICRI  Eco57MI
    Hin4I   HincII  HindIII     HpaI     MscI     MslI   MspA1I    PflMI
     PsrI     PstI    PvuII     SacI     SfcI     SmlI     StuI     StyI
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BsrBI    BsrGI   BssHII    BssSI    BstBI   BstEII
  BstZ17I     BtgI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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