(Circular) (Six-base) MAPSORT of: pF1KB8318.seq Check: 2665 from: 1 to: 3854 pF1KB8318 3854 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AccI GT'mk_AC Cuts at: 892 892 Size: 3854 Acc65I G'GTAC_C Cuts at: 307 401 870 307 Size: 94 469 3291 Fragments arranged by size: 3291 469 94 AclI AA'CG_TT Cuts at: 3694 3694 Size: 3854 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 302 818 1679 2111 2858 3126 302 Size: 516 861 432 747 268 1030 Fragments arranged by size: 1030 861 747 516 432 268 AflIII A'CryG_T Cuts at: 788 790 1206 2310 3287 788 Size: 2 416 1104 977 1355 Fragments arranged by size: 1355 1104 977 416 2 AgeI A'CCGG_T Cuts at: 2242 2242 Size: 3854 AleI CACnn'nnGTG Cuts at: 133 133 Size: 3854 AlwNI CAG_nnn'CTG Cuts at: 1159 2726 1159 Size: 1567 2287 ApaLI G'TGCA_C Cuts at: 2624 2624 Size: 3854 ApoI r'AATT_y Cuts at: 244 507 532 730 778 858 3207 3463 244 Size: 263 25 198 48 80 2349 256 635 Fragments arranged by size: 2349 635 263 256 198 80 48 25 AseI AT'TA_AT Cuts at: 20 20 Size: 3854 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3854 AvaI C'yCGr_G Cuts at: 874 874 Size: 3854 BamHI G'GATC_C Cuts at: 879 879 Size: 3854 BanI G'GyrC_C Cuts at: 307 401 870 1284 1532 1567 307 Size: 94 469 414 248 35 2594 Fragments arranged by size: 2594 469 414 248 94 35 BanII G_rGCy'C Cuts at: 868 1898 868 Size: 1030 2824 BbeI G_GCGC'C Cuts at: 1288 1536 1288 Size: 248 3606 BbsI GAAGACnn'nnnn_ Cuts at: 624 806 624 Size: 182 3672 BbvCI CC'TCA_GC Cuts at: 596 596 Size: 3854 BciVI GTATCCnnnnn_n' Cuts at: 1752 2513 3854 1752 Size: 761 1341 1752 Fragments arranged by size: 1752 1341 761 BclI T'GATC_A Cuts at: 1374 1374 Size: 3854 BglII A'GATC_T Cuts at: 1369 3406 1369 Size: 2037 1817 BlpI GC'TnA_GC Cuts at: 966 966 Size: 3854 Bme1580I G_kGCm'C Cuts at: 1479 1572 2628 1479 Size: 93 1056 2705 Fragments arranged by size: 2705 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 601 1066 1147 1482 3626 601 Size: 465 81 335 2144 829 Fragments arranged by size: 2144 829 465 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2221 2221 Size: 3854 Bpu10I CC'TnA_GC Cuts at: 596 596 Size: 3854 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 116 1030 1909 2401 2699 116 Size: 914 879 492 298 1271 Fragments arranged by size: 1271 914 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3558 3558 Size: 3854 BsaAI yAC'GTr Cuts at: 791 1207 791 Size: 416 3438 BsaBI GATnn'nnATC Cuts at: 6 1368 3405 6 Size: 1362 2037 455 Fragments arranged by size: 2037 1362 455 BsaHI Gr'CG_yC Cuts at: 1285 1533 2235 3731 1285 Size: 248 702 1496 1408 Fragments arranged by size: 1496 1408 702 248 BsaWI w'CCGG_w Cuts at: 1043 1564 2242 2516 2663 3074 1043 Size: 521 678 274 147 411 1823 Fragments arranged by size: 1823 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 300 330 375 405 300 Size: 30 45 30 3749 Fragments arranged by size: 3749 45 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 382 383 382 Size: 1 3853 BseYI C'CCAG_C Cuts at: 1279 2037 2614 3110 1279 Size: 758 577 496 2023 Fragments arranged by size: 2023 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1396 1442 2650 104 Size: 1292 46 1208 1308 Fragments arranged by size: 1308 1292 1208 46 BsiHKAI G_wGCw'C Cuts at: 868 1646 1836 2628 868 Size: 778 190 792 2094 Fragments arranged by size: 2094 792 778 190 Bsp1286I G_dGCh'C Cuts at: 868 1479 1572 1646 1836 1898 2628 868 Size: 611 93 74 190 62 730 2094 Fragments arranged by size: 2094 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3074 3074 Size: 3854 BspHI T'CATG_A Cuts at: 218 3030 218 Size: 2812 1042 BspMI ACCTGCnnnn'nnnn_ Cuts at: 904 1118 1420 1801 904 Size: 214 302 381 2957 Fragments arranged by size: 2957 381 302 214 BsrBI CCG'CTC Cuts at: 50 2146 2200 3684 3849 50 Size: 2096 54 1484 165 55 Fragments arranged by size: 2096 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1766 1766 Size: 3854 BsrFI r'CCGG_y Cuts at: 1852 2033 2242 1852 Size: 181 209 3464 Fragments arranged by size: 3464 209 181 BssSI C'ACGA_G Cuts at: 2125 2483 2125 Size: 358 3496 BstAPI GCAn_nnn'nTGC Cuts at: 1231 1231 Size: 3854 BstBI TT'CG_AA Cuts at: 2215 2215 Size: 3854 BstYI r'GATC_y Cuts at: 1 879 1346 1369 1704 1950 2962 3048 3060 Size: 878 467 23 335 246 1012 86 12 Cuts at: 3060 3406 1 Size: 346 449 Fragments arranged by size: 1012 878 467 449 346 335 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1804 1985 2148 1804 Size: 181 163 3510 Fragments arranged by size: 3510 181 163 BtsI GCAGTG_nn' Cuts at: 1097 1097 Size: 3854 DraI TTT'AAA Cuts at: 853 853 Size: 3854 DrdI GACnn_nn'nnGTC Cuts at: 1560 2418 1560 Size: 858 2996 EaeI y'GGCC_r Cuts at: 1439 1613 2004 2031 3376 1439 Size: 174 391 27 1345 1917 Fragments arranged by size: 1917 1345 391 174 27 EagI C'GGCC_G Cuts at: 1439 1439 Size: 3854 EarI CTCTTCn'nnn_ Cuts at: 838 1877 2087 838 Size: 1039 210 2605 Fragments arranged by size: 2605 1039 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2062 2372 2518 3664 2062 Size: 310 146 1146 2252 Fragments arranged by size: 2252 1146 310 146 EcoICRI GAG'CTC Cuts at: 866 866 Size: 3854 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 302 818 1679 2111 2858 3126 302 Size: 516 861 432 747 268 1030 Fragments arranged by size: 1030 861 747 516 432 268 EcoO109I rG'GnC_Cy Cuts at: 993 993 Size: 3854 EcoRI G'AATT_C Cuts at: 532 730 858 532 Size: 198 128 3528 Fragments arranged by size: 3528 198 128 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1873 1873 Size: 3854 FspI TGC'GCA Cuts at: 1635 1635 Size: 3854 HaeII r_GCGC'y Cuts at: 1288 1536 2558 3537 1288 Size: 248 1022 979 1605 Fragments arranged by size: 1605 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1696 1726 1728 1758 1696 Size: 30 2 30 3792 Fragments arranged by size: 3792 30 30 2 HincII GTy'rAC Cuts at: 264 893 264 Size: 629 3225 KasI G'GCGC_C Cuts at: 1284 1532 1284 Size: 248 3606 KpnI G_GTAC'C Cuts at: 311 405 874 311 Size: 94 469 3291 Fragments arranged by size: 3291 469 94 MluI A'CGCG_T Cuts at: 3287 3287 Size: 3854 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2525 2709 2525 Size: 184 3670 MscI TGG'CCA Cuts at: 1615 1615 Size: 3854 MslI CAynn'nnrTG Cuts at: 133 1843 1970 133 Size: 1710 127 2017 Fragments arranged by size: 2017 1710 127 MspA1I CmG'CkG Cuts at: 965 1279 1639 2652 2897 965 Size: 314 360 1013 245 1922 Fragments arranged by size: 1922 1013 360 314 245 NaeI GCC'GGC Cuts at: 2035 2035 Size: 3854 NarI GG'CG_CC Cuts at: 1285 1533 1285 Size: 248 3606 NgoMIV G'CCGG_C Cuts at: 2033 2033 Size: 3854 NspI r_CATG'y Cuts at: 907 1407 2314 907 Size: 500 907 2447 Fragments arranged by size: 2447 907 500 PciI A'CATG_T Cuts at: 2310 2310 Size: 3854 PmeI GTTT'AAAC Cuts at: 853 853 Size: 3854 PmlI CAC'GTG Cuts at: 791 791 Size: 3854 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2993 3025 2993 Size: 32 3822 PstI C_TGCA'G Cuts at: 901 1586 901 Size: 685 3169 PvuI CG_AT'CG Cuts at: 104 104 Size: 3854 PvuII CAG'CTG Cuts at: 1279 1639 1279 Size: 360 3494 RsrII CG'GwC_CG Cuts at: 2049 2049 Size: 3854 SacI G_AGCT'C Cuts at: 868 868 Size: 3854 SalI G'TCGA_C Cuts at: 891 891 Size: 3854 SapI GCTCTTCn'nnn_ Cuts at: 1877 2087 1877 Size: 210 3644 SbfI CC_TGCA'GG Cuts at: 901 901 Size: 3854 ScaI AGT'ACT Cuts at: 3371 3371 Size: 3854 SfcI C'TryA_G Cuts at: 33 897 1582 2575 2766 33 Size: 864 685 993 191 1121 Fragments arranged by size: 1121 993 864 685 191 SfoI GGC'GCC Cuts at: 1286 1534 1286 Size: 248 3606 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3854 SmaI CCC'GGG Cuts at: 876 876 Size: 3854 SmlI C'TyrA_G Cuts at: 131 1009 1924 2416 2678 131 Size: 878 915 492 262 1307 Fragments arranged by size: 1307 915 878 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3854 SphI G_CATG'C Cuts at: 907 1407 907 Size: 500 3354 StuI AGG'CCT Cuts at: 116 116 Size: 3854 StyI C'CwwG_G Cuts at: 397 988 1342 397 Size: 591 354 2909 Fragments arranged by size: 2909 591 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1437 1570 2029 2238 1437 Size: 133 459 209 3053 Fragments arranged by size: 3053 459 209 133 TatI w'GTAC_w Cuts at: 422 3369 422 Size: 2947 907 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3371 3403 3371 Size: 32 3822 Tth111I GACn'n_nGTC Cuts at: 1651 1651 Size: 3854 XbaI T'CTAG_A Cuts at: 885 885 Size: 3854 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1349 1349 Size: 3854 XmaI C'CCGG_G Cuts at: 874 874 Size: 3854 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI PmlI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BpmI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI