################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BfaI BglII BlnI BsaAI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstEII BstPI BstXI BstYI BstZ17I Cfr10I ClaI CpoI DraIII DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HpaI KasI KpnI MaeI MamI MfeI MflI MluI MluNI MroI MscI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp509I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccII 1314 CGCG CG^CG AflII 322 CTTAAG C^TTAAG AocI 781 CCTCAGG CC^T[ACGT]AGG BanI 583 GGCACC G^G[CT][GA]CC BfrI 322 CTTAAG C^TTAAG BlpI 160 GCTGAGC GC^T[ACGT]AGC Bpu1102I 160 GCTGAGC GC^T[ACGT]AGC BsiEI 1334 CGGCCG CG[GA][CT]^CG BsmBI 928 GAGACG CGTCTC(1/5) BspMI 621 GCAGGT ACCTGC(4/8) BssSI 588 CACGAG CACGAG(-5/-1) BstBI 424 TTCGAA TT^CGAA BstUI 1314 CGCG CG^CG Bsu36I 781 CCTCAGG CC^T[ACGT]AGG CelII 160 GCTGAGC GC^T[ACGT]AGC DraI 82 TTTAAA TTT^AAA EagI 1334 CGGCCG C^GGCCG EclXI 1334 CGGCCG C^GGCCG Eco52I 1334 CGGCCG C^GGCCG Eco81I 781 CCTCAGG CC^T[ACGT]AGG KspI 1313 CCGCGG CCGC^GG MaeII 607 ACGT A^CGT MvnI 1314 CGCG CG^CG NspV 424 TTCGAA TT^CGAA SacI 1182 GAGCTC GAGCT^C SacII 1313 CCGCGG CCGC^GG SapI 990 GAAGAGC GCTCTTC(1/4) SfuI 424 TTCGAA TT^CGAA SmaI 923 CCCGGG CCC^GGG XcmI 725 CCACCCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 923 CCCGGG C^CCGGG XmaI 923 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 566 GACGCC G[GA]^CG[CT]C 1434 GACGCC AspHI 1093 GAGCAC G[AT]GC[AT]^C 1182 GAGCTC AvaI 923 CCCGGG C^[CT]CG[GA]G 1177 CCCGAG BbvCI 700 GCTGAGG CCTCAGC(-5/-2) 769 GCTGAGG BclI 371 TGATCA T^GATCA 740 TGATCA BglI 838 GCCGCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1327 GCCGCCACGGC BsaHI 566 GACGCC G[GA]^CG[CT]C 1434 GACGCC BsaI 591 GAGACC GGTCTC(1/5) 892 GAGACC BsiHKAI 1093 GAGCAC G[AT]GC[AT]^C 1182 GAGCTC BsoBI 923 CCCGGG C^[CT]CG[GA]G 1177 CCCGAG BstAPI 829 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1229 GCAGCGTCTGC DsaI 1313 CCGCGG C^C[GA][CT]GG 1331 CCACGG EarI 109 GAAGAG CTCTTC(1/4) 990 GAAGAG FbaI 371 TGATCA T^GATCA 740 TGATCA HaeII 251 GGCGCT [GA]GCGC^[CT] 1069 AGCGCC Hin1I 566 GACGCC G[GA]^CG[CT]C 1434 GACGCC HindIII 227 AAGCTT A^AGCTT 319 AAGCTT Ksp632I 109 GAAGAG CTCTTC(1/4) 990 GAAGAG MseI 83 TTAA T^TAA 323 TTAA PflMI 544 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 729 CCAGGTCATGG TaqI 425 TCGA T^CGA 941 TCGA Tru9I 83 TTAA T^TAA 323 TTAA Tsp45I 266 GTGAC ^GT[GC]AC 1441 GTGAC TspRI 1142 CACTG CA[GC]TG[ACGT][ACGT]^ 1268 CACTG TthHB8I 425 TCGA T^CGA 941 TCGA Van91I 544 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 729 CCAGGTCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AfaI AluI AlwNI AvaII BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TseI